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SEREX clone BRC-Co-4 #1 (#RDB04481)

SEREX clone BRC-Co-4 #1

Clone info. SEREX clone BRC-Co-4, clone 1 from Colon Cancer AGH-CO-2. Reactivity: +. Corresponding gene: Suppression of Tumorigenesity 13 (colon carcinoma) (Hsp70 interacting Protein) (ST13) (NM_003932, 3214 bp).
Comment Similar clone: NY-REN-33 (ID 442), similarity: NY-REN-33 (ID 442) 99% similarity. UniGene cluster: , Ubiquitous. Nucleoide position 36 and later of NM_003932 is cloned.
Vector backbone pBK-CMV (Plasmid)
Size of vector backbone 4.5 kb
Selectable markers Kan^r
Gene/insert name Human Suppression of Tumorigenesity 13 (colon carcinoma) (Hsp70 interacting Protein) (ST13) (NM_003932, 3214 bp) cDNA
Depositor|Developer Obata, Yuichi |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04481 SEREX clone BRC-Co-4 #1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The SEREX clone BRC-Co-4 #1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04481).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB04481_B2Kop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,lacO,T3 pro,insert 5'
Sequence file: RDB04481_B2Koa.seq check
>04481_04481_B2Ko_3_CMV-Forward_B01_04_ABI24.ab1
    1 NNNNNNNNNN NTNNNNNNNN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
   61 AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCCGCAATTA CTGTGAGTTA GCTCACTCAT
  121 TAGGCACCCC AGGCTTTACA CTTTATACTT CCGGCTCGTA TATTGTGTGG AATTGTGAGC
  181 GGATAACAAT TTCACACAGG AAACAGCTAT GACCTTGATT ACGCCAAGCT CGAAATTAAC
  241 CCTCACTAAA GGGAACAAAA GCTGGAGCTC GCGCGCCTGC AGGTCGACAC TAGTGGATCC
  301 AAAGAATTCG GCACGAGGCT GTTCTGTCTT GCGGCAGCCG CCCCCTTCTG CGCGGTCACG
  361 CCGAGCCAGC GCCTGGGCCT GGAACCGGGC CGTAGCCCCC CCAGTTTCGC CCACCACCTC
  421 CCTACCATGG ACCCCCGCAA AGTGAACGAG CTTCGGGCCT TTGTGAAAAT GTGTAAGCAG
  481 GATCCGAGCG TTCTGCACAC CGAGGAAATG CGCTTCCTGA GGGAGTGGGT GGAGAGCATG
  541 GGTGGTAAAG TACCACCTGC TACTCAGAAA GCTAAATCAG AAGAAAATAC NAAGGAAGAA
  601 AAACCTGATA GTAAGAAGGT GGAGGAAGAN TTAAAGGCAG ACGAACNNTC AAGTGAGGAA
  661 AGTGNNCTAG AAANTGANNA NNNNNNGNGA NNGNNNNNGA NNCTNNNGCT NNNNNNNAAN
721 NNGNNNNNNN AANNNNNNNN NN
//
Primer: SV40polyA-R (Pr0109)
Region: T7 pro,insert 3'
Sequence file: RDB04481_B2Kob.seq check
>04481_04481_B2Ko_3_SV40polyA_R_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TCTACNAATG TGGTATGGCT GANNNNNATC
   61 ATGAACAGAC TGTGAGGACT GAGGGGCCTG AAATGAGCCT TGGGACTGTG AATCTAAAAT
  121 ACACAAACAA TTAGAATCAG TAGTTTAACA CATTATACAC TTAAAAATTG GATCTCCATT
  181 CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC
  241 GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
  301 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTGTA ATACGACTCA CTATAGGGCG
  361 AATTGGGTAC ACTTACCTGG TACCCCACCC GGGTGGAAAA TCGATGGGCC CGCGGCCGCT
  421 CTAGAAGTAC TCTCGAGTTT TTTTTTTTTT TTTTTTTTTT TTTTTTNNNN ANNANGANTT
  481 TNACTTTNAC CCNNTTNNGN NAANATTTGC AATATNNNAA NATANTTAGG TTAAATAAAT
  541 CAAATAAGGA ATCCNNAANA ANNNGNNCTN GAGTTAAGCC AAGTACNCNN NGGCNATTTC
  601 NAGAAAGGAN AANTCNNCNC NTTANNNAAA NANCNCNCNA GCTCAAANGN NNNTTTATNT
  661 GGNGANNGNT TTTNNNNNGN NTTNNAAANN CNNGNNNCNT CNNCANANNN NNNTTTNCNC
721 NTTNNNNNNA NNANNNNNNN NTNNNNNNNN NN
//
Primer: ST13-B_F (Pr1258)
Region: insert mid
Sequence file: RDB04481_B2Koc.seq check
>04481_04481_B2Ko_3_ST13-B_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNTTTNCN NGGGGAANGC CTGGTAATTT TCCCGGAGGA ATGCCTGGAA
   61 TGGGAGGGGG CATGCCTGGA ATGGCTGGAA TGCCTGGACT CAATGAAATT CTTAGTGATC
  121 CAGAGGTTCT TGCAGCCATG CAGGATCCAG AAGTTATGGT GGCTTTCCAG GATGTGGCTC
  181 AGAACCCAGC AAATATGTCA AAATACCAGA GCAACCCAAA GGTTATGAAT CTCATCAGTA
  241 AATTGTCAGC CAAATTTGGA GGTCAAGCGT AATGTCCTTC TGATAAATAA AGCCCTTGCT
  301 GAAGGAAAAG CAACCTAGAT CACCTTATGG ATGTCGCAAT AATACAAACC AGTGTACCTC
  361 TGACCTTCTC ATCAAGAGAG CTGGGGTGCT TTGAAGATAA TCCCTACCCC TCTCCCCCAA
  421 ATGCAGCTGA AGCATTTTAC AGTGGTTTGC CATTAGGGTA TTCATTCAGA TAATGTTTTC
  481 CTACTAGGAA TTACAAACTT TAAACACTTT TTAAATCTTC AAAATATTTA AAACAAATTT
  541 AAAGGGCCTG TTAATTCTTA TATTTTTCTT TACTANTCNT TTTGGANTTT TTTCTTTGAA
  601 TTATTGNCAN GGAATATACT TATGTATGGN AGANTACTGN TCTGANTGNA ATAAAAGTNA
661 TTANNGCGAN GNAAANNNNN NTNNNTTNNN NNNNAANTTN GNGNNNNNNN NNN
//
Primer: Poly(A) region primer #0 (Pr1055)
Region: insert mid
Sequence file: RDB04481_B2Kod.seq check
>04481_04481_B2Ko_3_poly(A)region#0_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NATTAGAATA NAATTAGGTT AAATAAATCA
   61 AATAAGGAAT CCAGAAGAAA CTGTTCTTGA GTTAAGCCAA GTACACACTG GCAATTTCGA
  121 GAAAGGAGAA CTCTACACAT TAACAAAAGA ACACACAAGC TCAAATGCAA GTTTATATGG
  181 TGAGTGTTTT TAAAGGATTT AAAAAGCATG CTCCATCAGC ATAATAGCTT TTTCTCATTT
  241 ATGTAACAGA TGCATTTCTG TCTGCTTGTG TACAGCAAAG GGTTAGAACC TGAATAAACT
  301 TATTTATGAT TTCAGAAATG TGGTATTTTA GAAATGTACT CCATAGAATT TACTTCACTG
  361 CTCCTACCAC TGATTCTTCA AATAAGCAAA GTACAGAAGG CCTTATCCCT TGAAAATGTT
  421 TTTAAACACT ANCACTTTAA ATTGAAACAC TGGTAGAAAC GTAGCAATGT CAGGTACAAT
  481 GCCTATACTT AGTCTTATTT AAAATAAGAA TTGAATGCAC AACAGAAAAT TTATCATTTT
  541 CTTTATCATT GGAGGGGAGG GAAAGAAAGC AAGCAGAGGT GGGGATATAG TTACACTATT
  601 TAAGAGCTGA ATACAAAGAC AATGAGTCTT TCATAGTCTC AACAACTCCA CAATGAATAA
  661 CTGTGGATTA TCTCATTTAT AATTCACATT GGATAAAAAA AATCGGTTCC CCTCTGTGTG
  721 TATCAATTTA ATTCAAACCT TATCTTCAGA ATACANAGAN GANNACTACA AGANAAACTA
  781 ACTGCNATAG GTTTTTCTAA GTANAAGANC NAACANNNAA GGGAATNCCN TCTTTCCAAT
  841 GTCTTCTGAN AAGGNAAGAN TTTGNNNNAA NNNNAANTCT NACNCTNNAA NNANCATTCT
  901 CNNNGNCNNN NTAANAAACT ACNNTTGNTG TGGNNATTAC CCTACNNTTA CCNTATGNNT
  961 NACNTATTNN NNTNAACNCN NCNNTNNNNN GNNNNNNNNN ANNANNNNNT NNNNANGANN
1021 NNNNNANNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB04481_B2Koe.seq check
>04481_04481_B2Ko_3_SV40pro_ori_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN CGNNGCCGCC TCNNCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
  121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
  181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
  241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
  301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC ANCTGTGCTC
361 GACGTTGNCN CTGANNCGNN NNNNNACTGN NNGCNATTGN NNNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles