Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAxCALNLhCD22(forward)

Shuttle vector to generate rAd expressing human CD22

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB05336_B1Isp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 (Pr0166) Region: lox P,insert 5'(5'UTR,CDS) Sequence file: RDB05336_B1Isa.seq
>05336_05336_B1Is_1_pAxCALNLF1_A02_02_ABI24.ab1     1 NNNNNNNNTN ANNNNGTCTG GNNNNNTCCG AACGCGTATA ACTTCGTATA GCATACATTA    61 TACGAAGTTA TCTCGAGTCG CTCGGTACGA TTTTCGAGCA CTGTTGGCCT ACTGGAGATG   121 CTGCCAGGGT CCCTGAAGAG GGAAGACACG CGGAAACAGG CTTGCACCCA GACACGACAC   181 CATGCATCTC CTCGGCCCCT GGCTCCTGCT CCTGGTTCTA GAATACTTGG CTTTCTCTGA   241 CTCAAGTAAA TGGGTTTTTG AGCACCCTGA AACCCTCTAC GCCTGGGAGG GGGCCTGCGT   301 CTGGATCCCC TGCACCTACA GAGCCCTAGA TGGTGACCTG GAAAGCTTCA TCCTGTTCCA   361 CAATCCTGAG TATAACAAGA ACACCTCGAA GTTTGATGGG ACAAGACTCT ATGAAAGCAC   421 AAAGGATGGG AAGGTTCCTT CTGAGCAGAA AAGGGTGCAA TTCCTGGGAG ACAAGAATAA   481 GAACTGCACA CTGAGTATCC ACCCGGTGCA CCTCAATGAC AGTGGTCAGC TGGGGCTGAG   541 GATGGAGTCC AAGACTGANA AATGGATGGA ACGAATACAC CTCAATGTCT CTGAAAGGCC   601 TTTTCCACCT CATATCCNGC TCCCTCCAGA AATTCAAGAG TCCCAGGAAG TCACTCTGAN   661 CTGCTNGCTG AATTTCTNCN GCTATGGGNN NCCNANCCNN NNGNANNGNN TCNNANNNGG 721 GGNNNNNNNN NN //
Primer: pCAGGS_R1 (Pr0614) Region: beta-globin polyA,insert 3'(polyA) Sequence file: RDB05336_B1Isb.seq
>05336_05336_B1Is_1_pCAGGS_R1_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCTA TTGNNNTGAA AATAAATTTC CTTTATTAGC CAGAAGTCAG    61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG   121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC   181 CTGAGGAGTG AATTGATTTT CGAGCACATG TGGCCTTTTT TTTTTTTTTT TTTTTTTTTT 241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT NNNNNNNNNN //
Primer: cd22-B_R (Pr1053) Region: insert mid (3'UTR,CDS) Sequence file: RDB05336_B1Isc.seq
>05336_05336_B1Is_1_ccd22-B_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTC TNCGNNNTNN GTGTGTGTGT GTGTGCGTGC GTGTGTGTGT    61 GTGTGTGCGC ACATGCGCGC AGGAGGAAGC CATGTGGCGG TGTCTGGGGA AACTCGGGGA   121 CTTCCCTGGC CCCCGCTGCC CCCAGTGCCT CTGCTGCAGC CCATCCAGTG TCAATGTTTG   181 AGGATCACAT AGTCCACATT TTCTTGTGCC TGAGGCCGCT CCCCGACCCC AAACTGGATC   241 AGCTCTGAGT AATGAATCCC CTCATCTTCT GGAAAATCTG GAATGACGTT CTCATAGTCG   301 CCCACTTGGC GCTTGTGCAA TGCTGAATAA GTGACCGTGT CATCGCAGTC CGGGGGAGGT   361 CTCTGCATCT CTGAGGACTC TGCATCTCCA GTTCGTGGTA TGTTCATCTC GGGAAAGCGC   421 AGGGTGGTGT AGCTAATGCC ATCTTCCATC ATTGGATTGT AGCATCCCAG GGAGTGGGGG   481 CCTTCAGAGA GGGGGGCCCT TCTAACCTTT TTATTCCTCA CAAAGAAGCT CTGGCCGCTG   541 GAATTCTCCT GAAGCCCCTG CTGGCTCTGT GTCCTCTTCC AACGTCGCTG GANCTTGAGC   601 CCACAGATTG CCAGGATGAN GANGGNGAGG CANGANCCGA GTCCCACAGC CACTCNCCTG   661 CCNATGGNCT CTGGGCTATA GTANACNGNG AGGGNGCTGA NANGNNAANN GNCCNTTNNC 721 NNNNNNNNNG NNCCCNNNNN NNNNN //
Primer: Poly(A)region primer#0 (Pr1055) Region: insert mid(3'UTR) Sequence file: RDB05336_B1Isd.seq
>05336_05336_B1Is_1_Poly(A)region primer#0_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNGNNNNCT CTGTCTCCCT GGCAGGTTAA TCCNCACCAG TGTGTCCATC    61 TCAAANCCNN NNTCCNCNNA NTCAAGGGGC CGCTGATACG TACGTCTCCC CGTGCAATGC   121 CTCACTTCCA AAGGAGAAGC CAGGCCAGGA CTGGGCCCCT TTCTACTTCG TTTCTGTCCT   181 TTTCTGGGCT TTCCCTCAGA GGCAGAAACT GGGCATAATA TTCGTGAGTG GGGATGTCAG   241 ATCAGGGGTA GAGGGCCAGA GAGATGGAGA AGGGAAGGAA AAATGATGAC GAGCAGATCC   301 CAGGAGGGAC ACAAGCAGCT GGGGGTGGAG ATGGCCAGTG GCCGTGGTGG GGGCAGGGGG   361 AGGTGTGCAT GTCAGGGCAG GTATTTAGAT GGATTTCTGA GTANCAAGGA GAGTGGAAGA   421 GAACAGGGGC AGGAGTTTTG GAGGTTTGGG GTTACCCTCA CCAAAAAGAC TGGCTCTGAG   481 CCAGGCACAA GGTTCTCCGC ANTGAGTGTG TGTGTGTGTG CGTGCGTGTG TGTGTGTGTG   541 TGCGCACATG CGCGCANGAN GAAGCCATGT GGCGGTGTCT GGNGAAACTC GNGGACTTCC   601 CTGGCCCCCG CTGCCCCCAG NGNNTCTGCT GNNNNCCATC NNNNGNNNNN GTTTGAGGNT   661 CNCNTANTCC NNNTTTTTCN TGNGCCNGAG GCCNNTCNCC NACCCCNAAC TNGNATCANC 721 TNNNANNANT GNANNCCNNN NTCNTCTNNN NNNNNNN //
Primer: phRLR1 (Pr0219) Region: AmpR pro,Ad5 psi Sequence file: RDB05336_B1Ise.seq
>05336_05336_B1Is_1_phRLR1_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNTTTGNNT GNNTTTAGAA AAATAAACAA ATAGGGGTTC    61 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT   121 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT TCAAGAATTC CGGATCCGGG   181 CCAATATGAT AATGAGGGGG TGGAGTTTGT GACGTGGCGC GGGGCGTGGG AACGGGGCGG   241 GTGACGTAGT AGTGTGGCGG AAGTGTGATG TTGCAAGTGT GGCGGAACAC ATGTAAGCGA   301 CGGATGTGGC AAAAGTGACG TTTTTGGTGT GCGCCGGTGT ACACAGGAAG TGACAATTTT   361 CGCGCGGTTT TAGGCGGATG TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG ATTTGGCCAT   421 TTTCGCGGGA AAACTGAATA AGAGGAAGTG AAATCTGAAT AATTTTGTGT TACTCATAGC   481 GCGTAATATT TGTCTAGGGC CGCGGGGACT TTGACCGTTT ACGTGGAGAC TCGCCCAGGT   541 GTTTTTCTCA GGTGTTTTCC GCGTTCCGGG TCAAAGTTGG CGTTTTATTA TTATAGTCAG   601 CATCGATTTA GCTNGNNCTG CNNNNCNAGG GNNCTCCATA AGAGANNAGG GACAGCTATG   661 ACTGGGNNTA NTCNGGANNN GANNAAAANC TGGCNNGTAA ANNTGNNNNN AAANTTTNNN 721 NNNTNANNAN NNANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.06.09

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