Resource data sheet
DNA Bank Top

CSII-EF-Venus (#RDB05552)

Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS (RDB04378) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB05552 CSII-EF-Venus DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-EF-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05552).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Sawada, M., PlexinD1 signaling controls domain-specific dendritic development in newborn neurons in the postnatal olfactory bulb. Front. Neurosci. 17: 1143130 (2023). PMID 37534039. [PubMed] [Article] [RRC of NBRP]
user_report Fukuda, T., Augmented Growth Hormone Secretion and Stat3 Phosphorylation in an Aryl Hydrocarbon Receptor Interacting Protein (AIP)-Disrupted Somatotroph Cell Line. PLoS One 11 (10): e0164131 (2016). PMID 27706259. [PubMed] [Article] [RRC of NBRP]
user_report Kimura, Y., CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Sci. Rep. 5: 10710 (2015) PMID 26039937. [PubMed] [Article] [RRC of NBRP]
user_report Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883. [PubMed] [Article] [RRC of NBRP]