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pPyZ-mJDP2_WT (#RDB05798)

Expression vector of mouse JDP2

Clone info. Expression vector driven by CAG promoter for mouse JDP2 wild-type.
Comment PCR product of mouse JDP2 was inserted. The primers were 5'-TCCTGCTATGATGCCTGGGCAGAT-3' and 5'-TCTCGAGTCACTTCTTGTCCAGCTGC-3'.
Vector backbone pPyCAGIRESzeocinpA (Plasmid)
Size of vector backbone 6.9 kb
Selectable markers Amp^r (E. coli), zeocin^r (mammalian cell)
Gene/insert name Mouse jun-dimerization protein 2 (JDP2) cDNA
Depositor|Developer Yokoyama, Kazunari |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cell Death Differ., 14, 1398-1405 (2007)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cell Death Differ., 14, 1398-1405 (2007))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05798 pPyZ-mJDP2_WT Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pPyZ-mJDP2_WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05798).

Reference section:

Nakade K., Pan J., Yoshiki A., Ugai H., Kimura M., Liu B., Li H., Obata Y., Iwama M., Itohara S., Murata T., Yokoyama K.K., JDP2 suppresses adipocyte differentiation by regulating histone acetylation. Cell Death Differ., 14, 1398-1405 (2007). PMID 17464331. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakade K., JDP2 suppresses adipocyte differentiation by regulating histone acetylation. Cell Death Differ., 14, 1398-1405 (2007). PMID 17464331. [link to RRC of NBRP]