Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsTR4 (#RDB06114)
Expression vector of human TR4.
| Alternative name | SET-0105_62 |
|---|---|
| Clone info. | Expression vector of human TR4 coding sequence. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR cloning, forward primer: atgaccagcccctccccacgcatccag; reverse primer: ctatagactggctccggtgatctggcca. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human TR4 cDNA |
| Depositor|Developer | DNA Bank, | |
| Sequence | RDB06114z.seq |
|---|---|
| Remarks, protocol and/or map (pdf) | RDB06114.pdf |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06114 | pCMFlag_hsTR4 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsTR4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06114). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12847_A4Gsp1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
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Primer: HIV-B_R (Pr0673) Region: HIV-1psi,5'LTR,CMVpro Sequence file: RDB17487_A9I6g.seq
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| 1 |
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Primer: BGH_rev Sequence file: RDB12847_A4Gsb_2.seq
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>06114_12847_A4Gs_BGHrev_H05_23.ab1 1 GTATCCTGTC CATTTAGGGT GAACTATAGA ATAGGGCCCT CTAGATGCAT GCTCGAGCGG 61 CCCTATAGAC TGGCTCCGGT GATCTGGCCA TTATACTCTG CTGTCTCCAT CTTGAGGATG 121 TAGGGGATTA TGCTGTCTAT CGAAACATTG CCAATGAGAC CAGTAAAAAA AAGTTCTTCT 181 GTTATGTTGG AGCTCATCAG CCTGAGTGCC GGCAGGCGAA CGAGGATCCG GGCCAATCGG 241 TAGGTGTCTT CTGAGTAGGT TTTCTGAACA TAGTCCTGCA ACTCCATCTG TGCCTTTTCT 301 TGGAATTTTT CAATCTGGCT TGTGCTGGTC AAACCTGGAT GATCGGGGCT AAAGAGAACT 361 ATAGCTTTAA GGTATGCATA CTCATAGCCA TCTATATCCA GCTTCGCCAT GCTGTTACAG 421 AACTCCTGCA GCTTCCAGAT GTGCTCCATG ACTTGCTTTA TCCGGTCACC AGAAAGTTTA 481 TCTTCCTGGA TGCTGTTCTG CAGGTGGTTG ACAATGGCAG CCAGGATGGT GGAGAGACTC 541 ATGACCTGGG CACACTGGGC CAGGCCGAGG GTGAAGAGCT CATTCCAGCA GGCCCGCACA 601 AGGCTGGTGT TGCAGTCCTG CCCAAGTGCC TGAAAGGCTG GGATTGACCG AGCCCAGTGC 661 ATTGAGAGGA AAAGCAGACG GGATGCAGAC TCACAGATGT AGTGCACGTT GAGGTACTCT 721 GGCATTGGAC TGGGCATTGT TAGCTTAAAT GTGACGTGTG TGTCTGAAAG GAGGGGGCCT 781 TCAACCTCAA TGATGGGTGT CGACTGGTCT CTGCTGATGA CGTGGATGCT CCCTCCTCCA 841 CTGGTGTCTA TCCCATCTGC CAAGCTTGGA GAAGAGGAGC TGTCTGTGGT ATTAAGTGCT 901 TTAGCTAAAG TATCAAATGC CCGAGTTATC TCACTTGCAG ACTGGTCCTC TGGCTGGATT 961 TCTGAAGTGT CACCGTTGTT CAAGATTCAC TTTAGTTTGG CAAGGGAGGT CACTACATTT 1021 GCCCAGTGTG CCCAGAGCTC CCTGGCTTGT TTCAGCCTTA GAAATCCGTA TAGGAGAACT 1081 GTACCATCTC ACGTATCAAA GCTGCTGGAT GTCACAGCCA TCCCCTTGTA TCAAGGAGGA 1141 CCTGTTGGTC CTTTGCCT // |
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Primer: SV40pro_F_V2 Sequence file: RDB12847_A4Gsc_2.seq
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>06114_12847_A4Gs_SV40pro_F_V2_A12_03.ab1 1 TAAGGCATTT GGAGCTAGGC TTTTGCAAAA GCTCCCGGGA GCTTGGATAT CCATTTTCGG 61 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC 121 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT 181 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT 241 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG 301 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG 361 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC 421 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC 481 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA 541 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA 601 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG 661 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG 721 TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC 781 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC 841 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG 901 GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC 961 GCCGCCCTTC TATGAAAGGT TGGGGCTTCG GAATCGTTTT CCCGGGACGC CCGGCTGGGA 1021 TGATCCTCCA GCGCGGGGGA TCTCATGGCT GCAGGTCTTC GCCCACCCCA ACTGCTTAAT 1081 GGCCAGCCTA ATTAATTGGT TAACGAAAAT TAAAAAGCCG ATAG // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human TR4
Reference sequence
Tips
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