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pDUAL2-HFG1 (#RDB06196)

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364062.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06196z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06196 pDUAL2-HFG1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-HFG1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06196).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14461_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14461_A6L7a.seq check
>06196_14461_A6L7_nmt1Fw_B02_05_ABI24.ab1
    1 CGGCATTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
   61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
  121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATATAT
  181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
  241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
  301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA
  361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA
  421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC
  481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC
  541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT
  601 TTTCAAAGAG TGCCATGCCC GAAGGCTATG TACAGGAAAG AACTATATTT TTCAAAGATG
  661 ACGGGGAACT ACAAGACACG TGCTGAAGTC AAAGTTTTGA AGGTGATACC CTTGTTAATA
  721 GAAATCGAGT TAAAAGGTAT TTGAATTTTA AAGAAAGATG GGAAACATTT CTTGGGACAC
  781 AAATTGGAAT ACAACTATTA ACTCACCACA ATTGTATACC ATCAATGGTC AGACAAAACC
  841 AAAAGAATTG GAATCAAAAG TTAACTTTCC AAAATTGAGT CACAACATTT GAAGAATGGA
  901 AAGCCGTTGC AACCTAGCAA GACCATTTAT CCACCAAAGT ACTTCGAATT GGGCGAATGC
  961 GCCCTGGTAC CTTTTTAACA AAACAACCAT TTTACCCTGA CCCATAGCAA TGCTGGCCAT
1021 TACAAAAGAT ATCCCAAACG AAAAAAAAAG CACTACTGAG GTC
//
Primer: ADH_rev
Sequence file: RDB14461_A6L7b.seq check
>06196_14461_A6L7_ADH_rev_C02_08_ABI24.ab1
    1 GCGAGAATCT CAGAATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT
  181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC
  241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA
  301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC
  361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT
  421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT
  481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT
  541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT
  601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG
  661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA
  721 CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAAGGGTAA GTTTTCCGTA TGTTGCATCA
  781 CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAAATTCAAC
  841 AAGAATTGGG ACACTCCAGT GAAAAGTTCT TTCTCCTTTA CCCATGGGTT TATCGTCATC
  901 ATCCCTTATA ATCCATATGA TGATGGTGGG TGATGCATAA GATCCAGGCC CTTGTCGACG
  961 CTAGCTTTAA ACAAAGCGAC TATAGTCAGA AAGTGAGATT GAGATGAAAT ATGACTTCCA
1021 CTATCATTGG ATGGAATATG CAGGAATCCG TCTTCCTTAA AAATCGTCCA AGT
//
Primer: ADH-Forward
Sequence file: RDB14461_A6L7c.seq check
>06196_14461_A6L7_ADH-Forward_D02_11_ABI24.ab1
    1 AACCTCCTCT TAATTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
   61 ACTAGTGCCT CTATTGATGC CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT
  121 TTGGAAGCTG ACGGTGTTCT TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT
  181 TGTCGTCCTG AGCAAGGTTT ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCAACCTT
  241 CGACCTTGCA ACTTTGCCAG CAAGTCTTTA GTCAAGTACA GCCCTTTGAA GCCTGAAATC
  301 GTTGAAGGTG TCGATTTTTG TGTTGTACGA GAACTTACTG GAGGTTGTTA CTTTGGTGAG
  361 CGCACTGAGG ACAACGGATC GGGTTATGCT ATGGACACTT GGCCTTACAG TTTGGAAGAA
  421 GTTTCTCGTA TTGCTCGTTT GGCGGCCGCG GGCCCAACCT TCCAATTCAT TAAATCGAGT
  481 CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT AAGTAAGAGA AGTTGTTAGT
  541 GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG ATCTCTTCTC CATTGTTTCC
  601 TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT ATTTTCTGAT TATTTTTTTC
  661 TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG TAAAATCGGG GTATGGTTAG
  721 TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT AGGGGCAAGC GAGACAGAAT
  781 GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG TATTTTAATT TGTATTTTTT
  841 TGTAATTTTA TTTCTCATTG TTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC
  901 GAAATGTCAA AAATAAGCTC AGCAAAATAT ACAATTTTAC GGGAAAGCGA TCAGCAACTT
  961 TATTCTCCGT TTGTAGATTC TTTTTTCTTC ATAAATTCCC AACATGTAAA CTGCCCATTT
 1021 GATTTTTTCT CTAGATGCCT AATAAATTGA AACTTATGTA TAATAATTAC ACAAGGTTTT
1081 GATAATGTAA ACTCTTACTC AGAAAAGGA
//
Primer: f1ori_R3
Sequence file: RDB14461_A6L7d.seq check
>06196_14461_A6L7_f1ori_R3_E02_14_ABI24.ab1
    1 GGGATGCTCT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
   61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
  121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
  181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG
  241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC
  301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG
  361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT
  421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT
  481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG
  541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG
  601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT
  661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC
  721 CAATTTTATA ATCAGGAAGT TTAAGTGAAT GGGTAGCACA TGTCGTAAAA ATTGTGAATT
  781 TTTATTGAAT AATATTTTTA AATACAAGCC CTTTTCTAGA CTAGGTATAC TCATAAACAT
  841 ATGAGCAAAA TGATAGAGGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT
  901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAGCC GCCCTCAGTT ACAAGAAAGA
  961 TTGCAAAAAT ATTGCCATCG TCATGGACTC AAGGTGCCAT ATAGCCATAT TTGAAGATTG
1021 ATAGTACTAA CTGCAGTTAG CGCG
//
Primer: Reverse2
Sequence file: RDB14461_A6L7e.seq check
>06196_14461_A6L7_Reverse2_F02_17_ABI24.ab1
    1 CGTACTCATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT
   61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC
  121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC
  181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT
  241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT
  301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC
  361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC
  421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC
  481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA
  541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT
  601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT
  661 TATGTAGTCG CTTTGAAAGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC
  721 GGTATGCTTC TCAAACCATT CTAAAGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC
  781 TTTGGGAAGA CATTTCAGCC CAAAAGCAAG AGACCACGTC CCAAGGTAAA CCAAACTTCT
  841 TTTGAGGCCT TGTATATACC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA
  901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTGA CGTATTTCAT GTCTGCGATT
  961 GCGATCTTCA AAGAATAAGA AAACGAATGC TTTTACTTAA GCCACAGTTT TCTACGATAT
1021 CTGCTCGTAT CTCGACACGG TCAATTGGTG CCTTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]
user_report Tanaka, T., Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins. Appl. Microbiol. Biotechnol., 97 (10): 4343-4352 (2013). PMID 23385477. [link to RRC of NBRP]