Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsPTEN

Expression vector of human PTEN.

Catalog number RDB06380
Resource name pCMFlag_hsPTEN
Alternative name SET-0175_1
Clone info. Expression vector of human PTEN. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC005821. PCR cloning, forward primer: atgacagccatcatcaaaga; reverse primer: tcagacttttgtaatttgtgtatgc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human PTEN1 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human PTEN1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06380 pCMFlag_hsPTEN DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06380.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB06380_A3Df.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB06380_A3Dfa.seq check
          >6380_6380_A3Df_T7long_C08_08.ab1
              1 CAAAGCCTGA ATTCACCATG GACTACAAGG ACGATGACGA TAAGGCGGCC ATGACAGCCA
             61 TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA TTCGACTTAG
            121 ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA AGACTTGAAG
            181 GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG CATAAAAACC
            241 ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA TTTAATTGCA
            301 GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT ATCAAACCCT
            361 TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA GCAATTCACT
            421 GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA CATCGGGGCA
            481 AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC AGAGACAAAA
            541 AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC CTGTTAAAGA
            601 ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT GAAACTATTC
          661 CAATGTTCAG TGGC
          //
          Primer:
          Sequence file: RDB06380_A3Dfb.seq check
          >6380_6380_A3Df_Sp6long_D08_11.ab1
              1 GCAGAAAACG ATCCTGGATG AATGCTCGAG CGGCCTCAGA CTTTTGTAAT TTGTGTATGC
             61 TGATCTTCAT CAAAAGGTTC ATTCTCTGGA TCAGAGTCAG TGGTGTCAGA ATATCTATAA
            121 TGATCAGGTT CATTGTCACT AACATCTGGT GTTACAGAAG TTGAACTGCT AGCCTCTGGA
            181 TTTGACGGCT CCTCTACTGT TTTTGTGAAG TACAGCTTCA CCTTAAAATT TGGAGAAAAG
            241 TATCGGTTGG CTTTGTCTTT ATTTGCTTTG TCAAGATCAT TTTTTGTTAA AGTAAGTACT
            301 AGATATTCCT TGTCATTATC TGCACGCTCT ATACTGCAAA TGCTATCGAT TTCTTGATCA
            361 CATAGACTTC CATTTTCTAC TTTTTCTGAG GTTTCCTCTG GTCCTGGTAT GAAGAATGTA
            421 TTTACCCAAA AGTGAAACAT TTTGTCCTTT TTTAGCATCT TGTTCTGTTT GTGGAAGAAC
            481 TCTACTTTGA TATCACCACA CACAGGTAAC GGCTGAGGGA ACTCAAAGTA CATGAACTTG
            541 TCTTCCCGTC GTGTGGGTCC TGAATTGGAG GAATATATCT TCACCTTTAG CTGGCAGACC
            601 ACAAACTGAG GATTGCAAGT TCCGCCACTG AACATTGGAA TAGTTTCAAA CATCATCTTG
          661 TGAAACAACA GTGCCACT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl