Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsPTEN (#RDB06380)
Expression vector of human PTEN.
| Alternative name | SET-0175_1 |
|---|---|
| Clone info. | Expression vector of human PTEN. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | Derived from Sugano BC005821. PCR cloning, forward primer: atgacagccatcatcaaaga; reverse primer: tcagacttttgtaatttgtgtatgc. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human PTEN1 cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06380.pdf |
|---|
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06380 | pCMFlag_hsPTEN | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsPTEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06380). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06380_A3Df.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
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Primer: Sequence file: RDB06380_A3Dfa.seq
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>6380_6380_A3Df_T7long_C08_08.ab1 1 CAAAGCCTGA ATTCACCATG GACTACAAGG ACGATGACGA TAAGGCGGCC ATGACAGCCA 61 TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA TTCGACTTAG 121 ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA AGACTTGAAG 181 GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG CATAAAAACC 241 ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA TTTAATTGCA 301 GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT ATCAAACCCT 361 TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA GCAATTCACT 421 GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA CATCGGGGCA 481 AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC AGAGACAAAA 541 AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC CTGTTAAAGA 601 ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT GAAACTATTC 661 CAATGTTCAG TGGC // |
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Primer: Sequence file: RDB06380_A3Dfb.seq
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>6380_6380_A3Df_Sp6long_D08_11.ab1 1 GCAGAAAACG ATCCTGGATG AATGCTCGAG CGGCCTCAGA CTTTTGTAAT TTGTGTATGC 61 TGATCTTCAT CAAAAGGTTC ATTCTCTGGA TCAGAGTCAG TGGTGTCAGA ATATCTATAA 121 TGATCAGGTT CATTGTCACT AACATCTGGT GTTACAGAAG TTGAACTGCT AGCCTCTGGA 181 TTTGACGGCT CCTCTACTGT TTTTGTGAAG TACAGCTTCA CCTTAAAATT TGGAGAAAAG 241 TATCGGTTGG CTTTGTCTTT ATTTGCTTTG TCAAGATCAT TTTTTGTTAA AGTAAGTACT 301 AGATATTCCT TGTCATTATC TGCACGCTCT ATACTGCAAA TGCTATCGAT TTCTTGATCA 361 CATAGACTTC CATTTTCTAC TTTTTCTGAG GTTTCCTCTG GTCCTGGTAT GAAGAATGTA 421 TTTACCCAAA AGTGAAACAT TTTGTCCTTT TTTAGCATCT TGTTCTGTTT GTGGAAGAAC 481 TCTACTTTGA TATCACCACA CACAGGTAAC GGCTGAGGGA ACTCAAAGTA CATGAACTTG 541 TCTTCCCGTC GTGTGGGTCC TGAATTGGAG GAATATATCT TCACCTTTAG CTGGCAGACC 601 ACAAACTGAG GATTGCAAGT TCCGCCACTG AACATTGGAA TAGTTTCAAA CATCATCTTG 661 TGAAACAACA GTGCCACT // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human PTEN1
Reference sequence
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