Please review the QC test results indicated by icon below as well as clone information before placing your order.

pREP41-ccdB2

S. pombe vector with nmt1 (medium strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12284_A5L2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12284_A5L2a.seq
>06520_12284_A5L2_nmt1Fw_E12_15.ab1     1 GAAAATTTTA TTAAATCAGG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC    61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT   121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG   181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT   241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT   301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT   361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA   421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC   481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT   541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA   601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG   661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT   721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC   781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT   841 GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA   901 TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCC TATTTCCCTA AAGGGTTTAT   961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACAGTTT TGATTTAACG  1021 TATCATATGG GACACTTTCT TCGCCCCCGT TTTCAACAAT GGGCAATATT ATACGCGGCC  1081 GACCAGTTGC TGATGGCGGC TGCGATTCAG TTCATCATGC CGATGTGAAT GCCATGCAAT 1141 GTTCGGCAGA ATGGCCTA //
Primer: nmt1Rv Sequence file: RDB12284_A5L2b.seq
>06520_12284_A5L2_nmt1Rv_F12_18.ab1     1 AGCGGCGGGG GCTCGAGATC TCATGATATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA    61 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA   121 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT   181 AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT   241 TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT   301 ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG   361 GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG   421 CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA   481 GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA   541 TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG   601 CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT   661 CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT   721 CATAGCATAC CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT TCTTATACCG   781 CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC GGATCCACGC   841 GTTTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG   901 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG   961 TCGCCTTGCG TATAATATTT GCCCATGGGT GAAAACGGGG GGCGAAGAAG TTGTCCATAT  1021 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA  1081 TATCTCAATA AAACCTTTAG GAATCGTAGC TTTTCACGTA CACGGCACAT CCTGCATCTA 1141 TTGTGTAGCA AGCCTTCCCA GA //
Primer: LEU2pro_F Sequence file: RDB12284_A5L2c.seq
>06520_12284_A5L2_LEU2pro_F_G12_21.ab1     1 ACTGCAGTGA ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT AGCAATCGTC    61 TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA TTTCAAGGAT   121 ATACCATTCT AATGTCTGCC CCTAAGAAGA TCGTCGTTTT GCCAGGTGAC CACGTTGGTC   181 AAGAAATCAC AGCCGAAGCC ATTAAGGTTC TTAAAGCTAT TTCTGATGTT CGTTCCAATG   241 TCAAGTTCGA TTTCGAAAAT CATTTAATTG GTGGTGCTGC TATCGATGCT ACAGGTGTTC   301 CACTTCCAGA TGAGGCGCTG GAAGCCTCCA AGAAGGCTGA TGCCGTTTTG TTAGGTGCTG   361 TGGGTGGTCC TAAATGGGGT ACCGGTAGTG TTAGACCTGA ACAAGGTTTA CTAAAAATCC   421 GTAAAGAACT TCAATTGTAC GCCAACTTAA GACCATGTAA CTTTGCATCC GACTCTCTTT   481 TAGACTTATC TCCAATCAAG CCACAATTTG CTAAAGGTAC TGACTTCGTT GTTGTCAGAG   541 AATTAGTGGG AGGTATTTAC TTTGGTAAGA GAAAGGAAGA CGATGGTGAT GGTGTCGCTT   601 GGGATAGTGA ACAATACACC GTTCCAGAAG TGCAAAGAAT CACAAGAATG GCCGCTTTCA   661 TGGCCCTACA ACATGAGCCA CCATTGCCTA TTTGGTCCTT GGATAAAGCT AATGTTTTGG   721 CCTCTTCAAG ATTATGGAGA AAAACTGTGG AGGAAACCAT CAAGAACGAA TTCCCTACAT   781 TGAAGGTTCA ACATCAATTG ATTGATTCTG CCGCCATGAT CCTAGTTAAG AACCCAACCC   841 ACCTAAATGG TATTATAATC ACCAGCAACA TGTTTGGTGA CATCATCTCC GATGAAGCCT   901 CCGTTATCCC AGGTTTCCTT GGGTTTGTTG CCATCTGCGT CTTGGCCTCT TTGCCAGACA   961 GACACCGCAT TGGTTTGTAC GACATGCCAC GGTCTGCTCA GATTGCTAAG AATAGTCACC  1021 TATCGCCACT ATCTTGTCTG CTGCATGATG GTGAATGTCA ATGGAACCTG GCTGGACACG 1081 TAGCCATGAA TGCAGTTAAA CGGTTGGCAA TGCAGGAAAT CGA //
Primer: f1ori_R3 Sequence file: RDB12284_A5L2d.seq
>06520_12284_A5L2_f1ori_R3_H12_24.ab1     1 GGTGGGATCT TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA    61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT   121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA   181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC CAACCTTCCA   241 ATTCATTAAA TCGAGTCCTC CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT   301 AAGAGAAGTT GTTAGTGATT TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT   361 CTTCTCCATT GTTTCCTTTC TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT   421 TCTGATTATT TTTTTCTATA GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA   481 ATCGGGGTAT GGTTAGTAGC GGCAGCGTTC ATTATGGTAT ATAAAAGATA AAAAGTAGGG   541 GCAAGCGAGA CAGAATGGGA TACAAGGGCA TCGTCTATAA TTATAGCTAA AAATTGTATT   601 TTAATTTGTA TTTTTTGTAA TTTTATTTCT CATTGTTTTA CTTAAAATGA ATGCGAATTA   661 GAGAAAACTT CAACGAAATG TCAAAATAAG CTCAGCAAAA TATACAATTT TAGGGAAAGC   721 GATCAGCAAC TTATTCTCCG TTGTAGATTC TTTTTCTTCA TAAATTCCAA CAATGTAACT   781 GCCAATTGAT TTTTCTCTAG ATGCTAATAA ATTGAAACTA ATGAATATAA TAACACAGGT   841 TTTGATAAAT TGTAAACTCT TACTCAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT   901 ATAAAGGCTC ATGGCGTTAA AGAGGATAGT AAAAATTCTA CAATTTTTTT AATTTAATGT   961 CACAATTGTC TTGCGAATAT AATTCTATTA ATTTTCGCTA ACTGCTGTTT TAGTACTATT  1021 TACTTTCATA TGCCCTTATA TGCAACTTTA ATTTCATGAG AATGCAATAT TTTTTGCAAT  1081 CTTTCTTGTA CTGAGCGCTT TTTTATCATT TAGTTACTCA TTATTTCATT TTTCAAGGTA 1141 AAGGGCCAAT TAAATT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl