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pHFF1 (#RDB06580)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06580z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06580 pHFF1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHFF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06580).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14494_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14494_A6L7a.seq check
>06580_14494_A6L7_nmt1Fw_A07_01_ABI24.ab1
    1 TGCATTTATT AATCAGGAAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
   61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
  121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATATA
  181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
  241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
  301 CTATGCATCA CCACCATCAT CATATGGATT ATAAGGATGA TGACGATAAA CCCATGGACT
  361 ACAAGGACGA CGATGACAAA GATATCATGA GATCTCGAGC CCGGGCGGCC GCTAACTAAC
  421 TAAGGGTAAA AGGAATGTCT CCCTTGCCAG TACTGCTAGG GTTTTTCTTT CAAACTATGG
  481 AAGCCCATTC AAGCTGCATA TTACGATTTT GTTTTTCGCT TTTAGAAAGT GGTTTAGATG
  541 AGATAATAGA AAAATTCTTG ATCTCCGACA ACGAGTACTT TTATTTTTTT TGCTAATCAC
  601 TTTACTCAAT ATTAGCTCGA AATCGTAGAA ACGTAGACGG GTGCGGGATA CCGAGTGGTG
  661 TAGTTAAGAA TTTTTATAAA CCACGTGGCC CAAAAATATG AACCCAAAAC GTTTATACAT
  721 GAGTATACTT TAAGAAGGCT ATACCCCTTC GTGTTAGATG TAGTTTTAGC TACCCAACCC
  781 GAGTCTATGA GCTTGACTTC AGATGTAGAA GGCATTAAAT CGTTTTGAAT ATTAATTAAA
  841 AAACGATGAA AATTAAATAT TTAAAAGCAA TCATACGCTG AAAATTTAGT GCTGTGGCTA
  901 ATCCTTCAAC ATGGAAATGC CATAAAAGTG ACTTTGACAA AAAAAAAAAG TATATACAGG
  961 TAGTAAACTC ATCTACTTTC ATTTGACTTT GTTTTACAGC ATGTGGAGGA GGATATTTTA
 1021 TTGCTAATCG TAGTTACATT CAATAGGTTA TACTATGAAA TTTCGACAGA TTGGCGCATG
1081 CATGCAAGAG CATTGTGCTT TTGGGGA
//
Primer: f1ori_R3
Sequence file: RDB14494_A6L7b.seq check
>06580_14494_A6L7_f1ori_R3_C07_07_ABI24.ab1
    1 GCGATGGTGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
  241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
  301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
  361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
  421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
  481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
  541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
  601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
  661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
  721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
  781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
  841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAGAATGTT AAAACTAATT TAACCTTTTT
  901 TATAAAGGCT CATGGCGTTA AAGAGGATAG TAAAAATTCT ACAATTTTTT TAAATTTAAT
  961 GTCACAATTG TCTTGCGAAT ATAATTCTAT TAAATTTTCG CTAACTGCTG TTTTAGTACT
 1021 ATTTACCTTT CATATGCCCT TATATGCAAA CTTTAGTCAT GGAGGAATGC AATTATTTTT
1081 GCATCGTTCA TGTACTGAGC GCTTTTTTAT CATTTTAAAG GTA
//
Primer: LEU2pro_F
Sequence file: RDB14494_A6L7c.seq check
>06580_14494_A6L7_LEU2pro_F_B07_04_ABI24.ab1
    1 AACCATTGCA GTTGAAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
   61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
  121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
  181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
  241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
  301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
  361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
  421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
  481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
  541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
  601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
  661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAGCTAATG
  721 TTTTGGCCTC TTCAAGATTA TGGAGAAAAA CTGTGGAGGA AACCATCAAG AACGAATTCC
  781 CTACATTGAA AGGTTCAACA TCAATTGATT GATTCTGCCG CCATGATCCT AGTTAAGAAC
  841 CCAACCCACC TAAATGGTAT TATAATCACC AGCAACATGT TTGGTGACAT CATCTCCGAT
  901 GAAGCCTCCG TTATCCCAGG TTTCCTTGGG TTTGTTGCCA TCTGCGTCCC TTGGGCCTCT
  961 TTGCCAGACA GACACGCAAT TTGGGTTTGT ACGAACCATG CCACGGGTCT GCTCCAGATT
 1021 TGCCAAGGAT TAGTTCACCT ATCGCCACTA TCTTGTCTGC CTGCCAATGA ATGTGAATGT
1081 CATGGACTTG CCTGAGAGTT AGCCATTGGT AG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]
user_report Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270. [link to RRC of NBRP]