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pHFF41 (#RDB06581)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06581z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06581 pHFF41 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHFF41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06581).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14495_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14495_A6L7a.seq check
>06581_14495_A6L7_nmt1Fw_D07_10_ABI24.ab1
    1 AGCATTTTAT TAAATCAGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
   61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
  121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
  181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
  241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCT
  301 ATGCATCACC ACCATCATCA TATGGATTAT AAGGATGATG ACGATAAACC CATGGACTAC
  361 AAGGACGACG ATGACAAAGA TATCATGAGA TCTCGAGCCC GGGCGGCCGC TAACTAACTA
  421 AGGGTAAAAG GAATGTCTCC CTTGCCAGTA CTGCTAGGGT TTTTCTTTCA AACTATGGAA
  481 GCCCATTCAA GCTGCATATT ACGATTTTGT TTTTCGCTTT TAGAAAGTGG TTTAGATGAG
  541 ATAATAGAAA AATTCTTGAT CTCCGACAAC GAGTACTTTT ATTTTTTTTG CTAATCACTT
  601 TACTCAATAT TAGCTCGAAA TCGTAGAAAC GTAGACGGGT GCGGGATACC GAGTGGTGTA
  661 GTTAAGAATT TTTATAAACC ACGTGGCCCA AAAATATGAA CCCAAAACGT TTATACATGA
  721 GTATACTTTA AGAAGGCTAT ACCCCTTCGT GTTAGATGTA GTTTTAGCTA CCCAACCCGA
  781 GTCTATGAGC TTGACTTCAG ATGTAGAAGG CATTAAATCG TTTTGAATAT TAATTAAAAA
  841 ACGATGAAAA TTAAATATTT AAAAGCAATC ATACGCTGAA AATTTAGTGC TGTGGCTAAT
  901 CCTTCAACAT GGAAATGCCA TAAAAGTGAC TTTGACAAAA AAAAAAAAGT ATATACAGGG
  961 TAGTAAACTC ATCTACTTCA TTGACTTTGT TTACAGCATG TGCATGAGAT ATTTATTGCT
 1021 AATCGTTAGT TACATTCATA AGTAATACTA ATGAAATCGA CAGATTGACG CATGCATGCA
1081 AGGAGCAATT TTGCTTGTGG GGGAGAAA
//
Primer: f1ori_R3
Sequence file: RDB14495_A6L7b.seq check
>06581_14495_A6L7_f1ori_R3_F07_16_ABI24.ab1
    1 GCCAATGGCG CCTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
  241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
  301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
  361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
  421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
  481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
  541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
  601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
  661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
  721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
  781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT ATACACAGGT
  841 TTTGATAAAT TGTAACTTCT TACTCAAAAA AAGATGTTAA AACTAATTTA ACCTTTTTTA
  901 TAAAGGCTCA TGGCGTTAAA GAGGATAGTA AAAATTCTAC ATTTTTATTT ATGTCACATT
  961 GTCTTGCAAT ATAATTCTAT ATTTCGCTAC TGCTGTTTAG TACTATTACT TACAATGGCT
 1021 TAATGGCACT TGATTCATGA GATGCCAATT TGCACTTTCT TGCACTGAGC CTTCTACCGT
1081 TAGGATC
//
Primer: LEU2pro_F
Sequence file: RDB14495_A6L7c.seq check
>06581_14495_A6L7_LEU2pro_F_E07_13_ABI24.ab1
    1 AAACCCTGAG TGACATATTA TTTAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC
   61 GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATTTCAAG
  121 GATATACCAT TCTAATGTCT GCCCCTAAGA AGATCGTCGT TTTGCCAGGT GACCACGTTG
  181 GTCAAGAAAT CACAGCCGAA GCCATTAAGG TTCTTAAAGC TATTTCTGAT GTTCGTTCCA
  241 ATGTCAAGTT CGATTTCGAA AATCATTTAA TTGGTGGTGC TGCTATCGAT GCTACAGGTG
  301 TTCCACTTCC AGATGAGGCG CTGGAAGCCT CCAAGAAGGC TGATGCCGTT TTGTTAGGTG
  361 CTGTGGGTGG TCCTAAATGG GGTACCGGTA GTGTTAGACC TGAACAAGGT TTACTAAAAA
  421 TCCGTAAAGA ACTTCAATTG TACGCCAACT TAAGACCATG TAACTTTGCA TCCGACTCTC
  481 TTTTAGACTT ATCTCCAATC AAGCCACAAT TTGCTAAAGG TACTGACTTC GTTGTTGTCA
  541 GAGAATTAGT GGGAGGTATT TACTTTGGTA AGAGAAAGGA AGACGATGGT GATGGTGTCG
  601 CTTGGGATAG TGAACAATAC ACCGTTCCAG AAGTGCAAAG AATCACAAGA ATGGCCGCTT
  661 TCATGGCCCT ACAACATGAG CCACCATTGC CTATTTGGTC CTTGGATAAA GCTAATGTTT
  721 TGGCCTCTTC AAGATTATGG AGAAAAACTG TGGAGGAAAC CATCAAGAAC GAATTCCCTA
  781 CATTGAAGGT TCAACATCAA TTGATTGATT CTGCCGCCAT GATCCTAGTT AAGAACCCAA
  841 CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGACATCATC TCCGATGAAG
  901 CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTTGGC CTCTTTGCCA
  961 GACAAGAACA CCGCATTTGG GTTTGTACGA ACCATGCCAC GGTTCTGCTC AGATTTGCAA
 1021 GATACGTCAA CCCTATCGCA CTATCCTGGT CTGCTGCCAT GAATGGTGAA AATGTCATTG
1081 ACTGGCCTGG AGAGTAAGGT CATTGAAGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]