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pEF-BOSneoSE-mNotch3 RAMIC (#RDB06773)

Expression vector of mouse Notch3 intracellular region

Alternative name #10037
Clone info. Expression vector of mouse Notch3 intracellular region.
Vector backbone pEF-BOS neo SE (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 3 cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06773 pEF-BOSneoSE-mNotch3 RAMIC DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneoSE-mNotch3 RAMIC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06773).

Reference section:

Kato, H., Sakai, T., Tamura, K., Minoguchi, S., Shirayoshi, Y., Hamada, Y., Tsujimoto, Y., Honjo, T., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06773_A3Ir.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEFF2
Sequence file: RDB06773_A3Ira.seq check
>06773_06773_A3Ir_pAxEFF2_A05_02.ab1
    1 AAATCTTTAT TTCCGGTGTC GTGAGGATTC TCTAGCCCAA CACCATGGAG CAAAAGCTCA
   61 TTTCTGAAGA GGACTTGAGA GAATTCAAGC GAGAACACAG CACCCTCTGG TTCCCTGAGG
  121 GTTTTGCATT ACACAAGGAC ATAGCTGCTG GCCACAAGGG CCGGAGGGAG CCTGTGGGAC
  181 AAGATGCACT GGGAATGAAG AACATGGCCA AGGGTGAGAG TCTGATGGGG GAGGTGGTCA
  241 CAGACTTGAA TGACTCAGAA TGTCCAGAGG CCAAGAGACT GAAGGTAGAG GAGCCGGGCA
  301 TGGGTGCAGA GGAGCCTGAG GACTGTCGCC AGTGGACCCA ACACCACCTG GTTGCTGCTG
  361 ATATCCGTGT GGCACCAGCC ACAGCGCTGA CTCCTCCTCA GGGAGATGCA GATGCAGACG
  421 GAGTGGATGT CAACGTCCGA GGGCCTGATG GCTTCACCCC ACTTATGCTG GCCTCCTTCT
  481 GTGGGGGAGC CCTGGAGCCG ATGCCAGCTG AGGAGGATGA GGCGGATGAC ACATCAGCCA
  541 GCATTATCTC AGATCTGATC TGTCAAGGGG CCCAGCTCGG GGCACGGACT GACCGCACTG
  601 GCGAGACCGC CCTGCATTTG GCTGCCCGCT ATGCTAGAGC GGATGCAGCC AAGCGTCTCC
  661 TGGATGCTGG GGCGGACACC AATGCCCAGG ATCATTCGGG CCGCACCCCT CTGCACACCG
  721 CAGTGACAGC TGATGCCCAG GGTGTCTTCC AGATTCTCAT CAGGAACCGC TCCACTGACC
  781 TGGATGCCCG AATGGCAGAT GGCTCTACTG CACTGATCCT GGCAGCCCGC CTGGCAGTGG
  841 AGGGCATGGT GGAAGAGCTC ATCGCCAGCC ATGCCGATGT CAATGCAGTG GATGAGCTTG
  901 GGAAATCTGC CTTACACTGG GCTGCAGCTG TGAACAACGT GGAGGCTACC TTGGCTCTGC
  961 TGAAAAATGG AGCCAACAAG GACATGCAGG ACAGCAAGGG AAGAGACGCC GCTGTTCTTG
 1021 GCCCGCTCGG GAGGGCAGCT ATGAGGCTGC CAAGCTGCTG CTGGATCATC TCGCCAACCG
 1081 GGAGATCACA GATCACTTTG GACAGGCCTG CCCCGGGACG TTGACCAGAG CCGCCTGCCA
1141 CCAGACATTG GTGGCGGGTT TGCCTGGGAA ACCAAGCCCC C
//
Primer: hGCSF-R
Sequence file: RDB06773_A3Irb.seq check
>06773_06773_A3Ir_hGCSF-R_B02_05.ab1
    1 CGTGGGCTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGGATAT CATCTAGACG
   61 GATCCGCAAT TGGCCCCATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
  121 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
  181 AGTGCCACCT GGATCCCCCT CAAATCCAAG AACTTAGGCC ATCACCTGCC TCTTGGGGGT
  241 AACTTCTGGT TGGGGTCCCA GCTGAGTCTG GGACTGAGGG AGGGAGGGAA CAGATATGGG
  301 GTGTGGCTGA GCAGGCAGGG CTCCAGAGGC TGTGGCATTG GTAGCAGTTG CTGGGCTAGG
  361 TGTTGAGTCA GACCAGTCTG AGAGGGATGG GGGGGATGGG CTGGCCCAGT GCTCTGGGGA
  421 CTCAGGAGAC GGGGTCAAAT AAGGATGCTC GCTGGGAACC CGCAGGAAGC GGGCCTTGGT
  481 GGGGCTACTG CGGGTTCCTG CTGCAGGATA TTCCTCTCCA TGTCCTGGAG CAGCCAGGTA
  541 GGGTGGGGGC CGCTCTTGGG GAGAAACTGG GGCTCCTGGG TTGAGCAACT GGGGTCCCGG
  601 AGCCAGGGGC AGCAGGAATG AGGGCCCTGG AGGGGCAGGT GGAGGCAGCC TGGCCCAGTC
  661 GAGGGGAACA GCTACAGGAT TGAGCAGACC AAAGCTGAGC ACACAGCCCC CAGGAGGCTG
  721 GCGCCCCAGA GGACCCGCCC TACTTGCGCC TAGCTGTGCC AAGGACACCG CGGTGGCCGT
  781 GGTGGCATAG GGGCCCTCCA AGGGGAAGCC TCCAGGGGAA GCAGGGGGCC CACTGAAAGG
  841 CCGTGGTGAG TCCAGAGAGT CCACCGGTGA CAGTGTGACA GAGCTGTCTG CCAGAGGTCC
  901 TGGGACAGGC TAGTGTCAGC TTCTTGCCCC GACCACGAGT TCCCTGTGGC CCCAGCCCGG
  961 TCTTGCCAGG TGGCCTCCTG CTCTTCTTGG TCCCAGACTG CACCGCTTTG AGGCCAGGAA
 1021 GGAAGGCCCC TGGTGGGCAG AGCAATGGCC CTAAGCCATG GGGACCAGAG GGACTTCGAG
 1081 GTCCACTGGC TGGTCCAGCA ACCGCACATG TCTGATGCAG CCGCTCCTGG GCCACGTCCG
 1141 GGGCCAGCTT GTTCCAAGTG ATCTGTGAAT CTCCGTGGGC GAGAATTGAA TTCCAGCAGA
1201 ACAGGACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]