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pGa981-6 (#RDB06776)

Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter. TP1-luciferase reporter.

Alternative name TP1-luc(981-6) , #9507
Clone info. Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter.
Comment The response element was inserted in front of the mnimal beta-globin promoter of pGa50-7. Please refer Fig.1 of Oswald, F., Mol. Cell. Biol. 21 (22): 7761-7774 (2001).
Vector backbone pGa50-7 (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Artifical EBNA2 response element of the TP-1 synthesized
Depositor|Developer Honjo, Tasuku |
 
Sequence (full) RDB06776hts01.seq checkAssembled from experimentally sequenced data.
Remarks, protocol and/or map (pdf) RDB06776.pdf
Publication Kurooka, H., Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771. [link to RRC of NBRP]
Publication Minoguchi, S., Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338. [link to RRC of NBRP]
Test sheet RDB06776B0p1-1.pdf 
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res., 26, 5448-5455 (1998).). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nucleic Acids Res., 26, 5448-5455 (1998).)。 公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06776 pGa981-6 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGa981-6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06776).

Reference section:

Kurooka, H., Kuroda, K., Honjo, T., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771. [link to RRC of NBRP]
Minoguchi, S., Taniguchi, Y., Kato, H., Okazaki, T., Strobl, L.J., Zimber-Strobl, U., Bornkamm, G.W., Honjo, T., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kurooka, H., Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region. Nucleic Acids Res., 6(23): 5448-5455 (1998). PMID 9826771. [link to RRC of NBRP]
original Minoguchi, S., RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol., 17, 2679-2687 (1997). PMID 9111338. [link to RRC of NBRP]
reference Itatani, Y., Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 159 (1): 133-140 (2016). PMID 26229111. [link to Journal]
reference Shimizu, K., Integrity of intracellular domain of Notch ligand is indispensable for cleavage required for release of the Notch2 intracellular domain. EMBO J. 21 (3): 294-302 (2002). PMID 11823422. [link to Journal]
reference Oswald, F., p300 acts as a transcriptional coactivator for mammalian Notch-1. Mol. Cell. Biol. 21 (22): 7761-7774 (2001). PMID 11604511. [link to Journal]
user_report Carter, A.A., Structural features of the Notch ankyrin domain-Deltex WWE2 domain heterodimer determined by NMR spectroscopy and functional implications. Structure 31 (5): 584-594.e5 (2023). PMID 36977409. [link to RRC of NBRP]
user_report Kawamoto, A., Ubiquitin D is up-regulated by synergy of Notch signalling and TNF-alpha in the inflamed intestinal epithelia of IBD patients. J. Crohns Colitis 13 (4): 495-509 (2019). PMID 30395194. [link to RRC of NBRP]
user_report Yoneyama, T., Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells. J. Nat. Prod. 80 (9): 2453-2461 (2017). PMID 28817274. [link to RRC of NBRP]
user_report Sugiyama, M., Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol. Rep. 33 (3): 1123-1130 (2015). PMID 25522823. [link to RRC of NBRP]
user_report Matsuda, M., Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Nat. Commun. 6: 6195 (2015). PMID 25652697. [link to RRC of NBRP]
user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448. [link to RRC of NBRP]