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pCMFlag_hsE2F4

Expression vector of human E2F4.

Catalog number RDB06786
Resource name pCMFlag_hsE2F4
Alternative name SET-0113_9-1
Clone info. Expression vector of human E2F4. Expression has not yet been confirmed.
Comment PCR cloning, forward primer: gcggaggccgggccacaggc; reverse primer: tcagaggttgagaacaggcacatc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human E2F transcription factor 4,p107/p103-binding cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human E2F transcription factor 4,p107/p103-binding
          Reference sequence
            

          Distribution information

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          RDB06786 pCMFlag_hsE2F4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12837_A4Hb.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12837_A4Hba.seq check
          >06786_12837_A4Hb_CMV-Foward_C07_07.ab1
              1 CGTTCCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGAATTCAC CATGGACTAC AAGGACGATG ACGATAAGGC
            181 GGCCGCGGAG GCCGGGCCAC AGGCGCCGCC GCCCCCGGGC ACTCCAAGCC GGCACGAAAA
            241 GAGCCTGGGA CTGCTCACCA CCAAGTTCGT GTCCCTTCTG CAGGAGGCCA AGGACGGCGT
            301 GCTTGACCTC AAGCTGGCAG CTGACACCCT AGCTGTACGC CAGAAGCGGC GGATTTACGA
            361 CATTACCAAT GTTTTGGAAG GTATCGGGCT AATCGAGAAA AAGTCCAAGA ACAGCATCCA
            421 GTGGAAGGGT GTGGGGCCTG GCTGCAATAC CCGGGAGATT GCTGACAAAC TGATTGAGCT
            481 CAAGGCAGAG ATCGAGGAGC TGCAGCAGCG GGAGCAAGAA CTAGACCAGC ACAAGGTGTG
            541 GGTGCAGCAG AGCATCCGGA ACGTCACAGA GGACGTGCAG AACAGCTGTT TGGCCTACGT
            601 CACTCATGAG GACATCTGCA GATGCTTTGC TGGAGATACC CTCTTGGCCA TCCGGGCCCC
            661 ATCAGGCACC AGCCTGGAGG TGCCCATCCC AGAGGGTCTC AATGGGCAGA AGAAGTACCA
            721 GATTCACCTG AAGAGTGTGA GTGGTCCCAT TGAGGTTCTG CTGGTGAACA AGGAGGCATG
            781 GAGCTCACCC CCTGTGGCTG TGCCTGTGCC ACCACCTGAA GATTTGCTCC AGAGCCCATC
            841 TGCTGTTTCT ACACCTCCAC CTCTGCCCAA GCCTGCCCTA GCCCAGTCCC AGGAAGCCTC
            901 ACGTCCAAAT AGTCCTCAGC TCACTCCCAC TGCTGTCCCT GGCAGTGCAG AAGTCCAGGG
            961 AATGGCTGGG CCCAGCAGCT GAGATCACAG TGAGTGGCGG GCCCTGGGAC TGATAGCAAA
           1021 GGGACAGTGG TGAGCTCAGT TCACTCCCCA CTGGGCCCCA ACACACTGGA CACCCGGGCA
           1081 CTGCAGTCTT CTGCCTTGCT TGGACAGCCA GCCAGCCAGG CAGCAAGGCA GGCCAGGCCA
          1141 GG
          //
          Primer: BGH_rev
          Sequence file: RDB12837_A4Hbb.seq check
          >06786_12837_A4Hb_BGHrev_D07_10.ab1
              1 GCCTTACCCA TTAGGGTGAA CTATAGAATA GGGCCCTCTA GATGCATGCT CGAGCGGCTC
             61 AGAGGTTGAG AACAGGCACA TCAAAGAGGT CACAGACACC TTCACTCTCG TCCAGGTTGT
            121 AGATATAATC GTGGTCTCCC GGGGGTGGAG AAAGACGAAG CAGAGGGGCA AACACTTCTG
            181 AGGACATCAA CTCCTCCAGC AGCTCCGAGC TCATGCACTC TCGTGTGGGA TCAAAGATTT
            241 CTGACAGCTC TTTGGGGAGT TCCAAAACAC CTGTGGGGTC TGCCTTGATG GGCTCAAAGG
            301 AGGTAGAAGG GTTGGGTCCG GACGAACTGC TGCTGTTACT GTTGCTGCTG CTGCTGCTGC
            361 TGCTGCTGCT GCTGCTGCTG CTGTCCAGCA GGGCAGAAGA CTGCAGTGGC CGGGTGTCCA
            421 GTGTTGTTGG GCCCAGTGGG AGTGAACTGA GCTCACCACT GTCCTTGCTA TCAGTCCCAG
            481 GGCCGCCACT CACTGTGATC TCAGCTGCTG GGCCAGCCAT TCCCTGGACT TCTGCACTGC
            541 CAGGGACAGC AGTGGGAGTG AGCTGAGGAC TATTTGGACG TGAGGCTTCC TGGGACTGGG
            601 CTAGGGCAGG CTTGGGCAGA GGTGGAGGTG TAGAAACAGC AGATGGGCTC TGGAGCAAAT
            661 CTTCAGGTGG TGGCACAGGC ACAGCCACAG GGGGTGAGCT CCATGCCTCC TTGTTCACCA
            721 GCAGAACCTC AATGGGACCA CTCACACTCT TCAGGTGAAT CTGGTACTTC TTCTGCCCAT
            781 TGAGACCCTC TGGGATGGGC ACCTCCAGGC TGGTGCCTGA TGGGGCCCGG ATGGCCAAGA
            841 GGGTATCTCC AGCAAAGCAT CTGCAGATGT CCTCATGAGT GACGTAGGCC AAACAGCTGT
            901 TCTGCACGTC CTCTGTGACG TTCCGGATGC TCTGCTGCAC CCACACCTTG TGCTGGTCTA
            961 GTTCTTGCTC CCGCTGCTGC AGCTCCTCGA TCTCTGCCTT GAGCTCAATC AGTTGTCAGC
           1021 AAATCTCCCG GGTATTGCAG CCCAGGGCCC CACACCCTTT CACTGGAATG CTGGTTCTTG
           1081 GACTTTTTTC TCGATTAGCC CGGATTACCC TCCAAACATG GTTAATGTTC GTAAATCCCG
          1141 TCCGCGCTTC CTTGC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl