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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsCEBPB (#RDB07002)

Expression vector of human CREBPB.

Alternative name SET-0013_37
Clone info. Expression vector of human CREBPB. Expression has not yet been confirmed.
Comment Derived from Sugano BC007538. PCR cloning, forward primer: atgcaacgcctggtggcctg; reverse primer: ctagcagtggccggaggagg.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CEBPB/NF-IL-6 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07002 pCMFlag_hsCEBPB DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsCEBPB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07002).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07002_09Dnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert 5'
Sequence file: RDB07002_09Dna.seq check
>07002_07002_B0FmF_CMV-Forward_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG
  181 GCGGCCATGC AACGCCTGGT GGCCTGGGAC CCAGCATGTC TCCCCCTGCC GCCGCCGCCG
  241 CCTGCCTTTA AATCCATGGA AGTGGCCAAC TTCTACTACG AGGCGGACTG CTTGGCTGCT
  301 GCGTACGGCG GCAAGGCGGC CCCCGCGGCG CCCCCCGCGG CCAGACCCGG GCCGCGCCCC
  361 CCCGCCGGCG AGCTGGGCAG CATCGGCGAC CACGAGCGCG CCATCGACTT CAGCCCGTAC
  421 CTGGAGCCGC TGGGCGCGCC GCAGGCCCCG GCGCCCGCCA CGGCCACGGA CACCTTCGAG
  481 GCGGCTCCGC CCGCGCCCGC CCCCGCGCCC GCCTCCTCCG GGCAGCACCA CGACTTCCTC
  541 TCCGACCTCT TCTCCGACGA CTACGGGGGC AAGAACTGCA AGAAGCCGGC CGAGTACGGC
  601 TACGTGAGCC TGGGGCGCCT GGGGGCCGCC AAGGGCGCGC TGCACCCCGG CTGCTTCGCG
  661 CCCCTGCACC CACCGCCCCC GCCGCCGCCG CCGCCCGCCG AGCTCAAGGC GGANCCGGGC
  721 TTCGAGCCCG CGGACTGCAA GCGGANNGAN GANGCCGGGG CGCCGGGCGG CGGCGCANGC
  781 ATGGCGGCGG GCTTCCCGTA CGCGCTGCGC GCTTACCTCG GCTACCAGGC GGNGCCGANC
  841 GGCAGCAGCG GGNNCCTCTC CACGTCCTCC TCNNNCAGCC NGCCCGGCAC GCCNNNCCCN
  901 GCNNANGCCN ANGNCGCCCC CCNACCGCCN GCNACNNNGG GGNNNNNNCN NNNGCCNNNN
961 NNNNNCNNNN NCNANNNCCN ANNANNNNNN NNNN
//
Primer: BGH_rev2 (Pr0606)
Region: BGH pA, insert 3'
Sequence file: RDB07002_09Dnb.seq check
>07002_07002_B0FmF_BGH_rev2_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN GGNNGGGCAN ACNACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA
  121 TGCATGCTCG AGCGGCCCTA GCAGTGGCCG GAGGAGGCGA GCAGGGGCTC GGGCAGCTGC
  181 TTGAACAAGT TCCGCAGGGT GCTGAGCTCG CGCGACAGCT GCTCCACCTT CTTCTGCAGC
  241 CGCTCGTTCT CGGCCGTGAG CTCCAGGACC TTGTGCTGCG TCTCCAGGTT GCGCATCTTG
  301 GCCTTGTCGC GGCTCTTGCG CACGGCGATG TTGTTGCGCT CGCGCCGGAT CTTGTACTCG
  361 TCGCTGTGCT TGTCCACGGT CTTCTTGGCC TTGCTCTTGA CCTGCGAGGG CGCCGGCGCG
  421 GCCCCCGCGT AGCAGGCGGT CGGGGGCGCC TTGGCGTCAG CGGGGCTCGG CGTGCCGGGC
  481 GGGCTGGACG AGGAGGACGT GGAGAGGCTC CCGCTGCTGC CGCTCGGCAC CGCCTGGTAG
  541 CCGAGGTAAG CGCGCAGCGC GTACGGGAAG CCCGCCGCCA TGCCTGCGCC GCCGCCCGGC
  601 GCCCCGGCCT CCTCCTTCCG CTTGCAGTCC GCGGGCTCGA AGCCCGGCTC CGCCTTGAGC
  661 TCGGCGGGCG GCGGCGGCGG CGGGGGCGGT GGGTGCAGGG GCGCGAAGCA GCCGGGGTGC
  721 AGCGCGCCCT TNGCGGCCCC CAGGCGCCCC AGGCTCACGT AGCCGTACTC GGCCGGCTTC
  781 TTGCAGTTCT TGCCNNCGTA NTCGTCGGAN AAGANGTCGG ANANGAANTC GTGGNGCTGC
  841 CCGGNNGANG CGGNCGCGGG GGNCNGGCGC NGGCGGNNCC GNNNCNAANG NGTNCGNGGN
  901 CGNNGCGGNN GCNNNGGNNN NNCNGNGCGN NNNGCNGNNN NNNNNACNGG NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR
Sequence file: RDB07002_09Dnc.seq check
>07002_07002_B0FmF_SV40pro_ori_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CCNCNGCCTC TGNGCTNTTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGGATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGANGAT CTCGTCGTGA CCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
  781 CCGGCTGGGT GTGGCGGANC GCTATCANGN NATAGCGTTG GCTACCCGTG ATATTGCTGA
  841 AGANCTTGGC GGCGAANNNN CTGNNNNCTT CCNCGNGCTT NACNGNATCG CCGCTCCNNA
  901 TTCGCANCGN NNNGNCNNNN NNNCGNCNNN NNNGNNNANN TNNNNNNNNN CGGGNNNNNN
961 GGGNNTNNNN ANNNNNCNNN NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles