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pCMFlag_hsCREB2 (#RDB07012)

Expression vector of human CREB2

Alternative name SET-0022_1
Clone info. Expression vector of human CREB2. Derived from Sugano AK057751/CBL01850 clone.
Comment Expression has not yet been confirmed. PCR cloning, forward primer: ATGACCGAAATGAGCTTCCTG; reverse primer: CTAGGGGACCCTTTTCTTCC. Known difference: A to G substitution at 1027nt of NM_182810.2 (Pro, synonym).
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37C, LB+Amp
Gene/insert name Human CREB2; ATF4 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07012.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
Additional terms and conditions:
Regrding pRc/CMV:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
付加的使用条件：
pRc/CMVについて:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog # Resource name Shipping form Fee
RDB07012 pCMFlag_hsCREB2 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsCREB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07012).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07012_A6Cip1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: FLAG,insert5'
Sequence file: RDB07012_A6Cia.seq check
>07012_07012_A6Ci_CMV-Forward_D05_11.ab1
    1 ATTTATAACC GTCCGGCCCC ATTTGAACGC AAAATGGGCG GTAGGCGTGT ACGGTGGGAG
   61 GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGGA
  121 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT
  181 GACGATAAGG CGGCCATGAC CGAAATGAGC TTCCTGAGCA GCGAGGTGTT GGTGGGGGAC
  241 TTGATGTCCC CCTTCGACCA GTCGGGTTTG GGGGCTGAAG AAAGCCTAGG TCTCTTAGAT
  301 GATTACCTGG AGGTGGCCAA GCACTTCAAA CCTCATGGGT TCTCCAGCGA CAAGGCTAAG
  361 GCGGGCTCCT CCGAATGGCT GGCTGTGGAT GGGTTGGTCA GTCCCTCCAA CAACAGCAAG
  421 GAGGATGCCT TCTCCGGGAC AGATTGGATG TTGGAGAAAA TGGATTTGAA GGAGTTCGAC
  481 TTGGATGCCC TGTTGGGTAT AGATGACCTG GAAACCATGC CAGATGACCT TCTGACCACG
  541 TTGGATGACA CTTGTGATCT CTTTGCCCCC CTAGTCCAGG AGACTAATAA GCAGCCCCCC
  601 CAGACGGTGA ACCCAATTGG CCATCTCCCA GAAAGTTTAA CAAAACCCGA CCAGGTTGCC
  661 CCCTTCACCT TCTTACAACC TCTTCCCCTT TCCCCAGGGG TCCTGTCCTC CACTCCAGAT
  721 CATTCCTTTA GTTTAGAGCT GGGCAGTGAA GTGGATATCA CTGAAGGAGA TAGGAAGCCA
  781 GAACTACACT GCTTACGTTG CCATGATCCC TCAGTTGCAT AAAGGAAGGA GAACCCCCCT
841 TCA
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A),insert3'
Sequence file: RDB07012_A6Cib.seq check
>07012_07012_A6Ci_BGH_rev2_E05_14.ab1
    1 GCCCCGCGGA GTTCAGAAGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGAGCTCTAG CATTTAGGTG ACACTATAGA ATAGGGCCCT
  121 CTAGATGCAT GCTCGAGCGG CCCTAGGGGA CCCTTTTCTT CCCCCTTGCC TTGCGGACCT
  181 CTTCTATCAA ATCTTTCAGG TACTGGATCT CCTTGGCCAG GGAATCCGCC CTCTCTTTTA
  241 GAGCCTCGTT CTTCTTTTCC AGCTCTTTGC ACTCACCAGT AAGAGCCTCC TGCTCCGCCC
  301 TCTTCTTCTG GCGGTACCTA GTGGCTGCTG TCTTGTTTTG CTCCATTTTT TTCAGCTTCT
  361 TATCCAGTTT CTCACCCTTT ACTTTTGCTG CTACCATCTT CTCTCCAGGA GGATCGTAAG
  421 GTTTGGGACG GGCAGACCCA CAGAGAACAC CCGGAGATGG GAGGCTCCTA TTTGGAGAGC
  481 CCCTGGTAGA GGGGCTGTGC TGAGGAGACC CCAGATAGGA CTCTGGGCTC ATACAGATGC
  541 CACTATCATT ATCTGAAGGG GTGTCTTCCT CCTTTATGCA CTGAGGGATC ATGGCAACGT
  601 AAGCAGTGTA GTCTGGCTTC CTATCTCCTT CAGTGATATC CACTTCACTG CCCAGCTCTA
  661 AACTAAAGGA ATGATCTGGA GTGGAGGACA GGACCCCTGG GGAAAGGGGA AGAGGTTGTA
  721 AGAAGGTGAA GGGGGCAACC TGGTCGGGTT TTGTTAAACT TTCTGGGAGA TGGCCAATTG
  781 GGTTCACCGT CTGGGGGGGC TGCTTATTAG TCTCCTGGAC TAGGGGGGCA AAGAGATCAC
  841 AAGTGTCATC CAACGTGGTC AGAAGGTCAT CTGGCATGGT TTCCAGGTCA TCTATACCCA
  901 ACAGGGCATC CAAGTCGAAC TCCTTCAAAT CCATTTTCTC CAACATCCAA TCTGTCCCGG
  961 AGAAGGCATC CTCCTTGCTG TTGTTGGAGG ACTGACCAAC CCATCCACAG CCAGCCATTC
 1021 GGGAGGAGCC CGCCTTTAGC CTTGTCGCTG GAGAACCATG AGTTTGAAGT GCTTTGGCCA
 1081 CCTCCAGGTA TTCATCTAGA GACTAGCTTT CATCAGCTCG AACCCGAACT GATCGAGGGG
1141 GAACATCAAG TCCCCTCACC GAACAACCTT CGCT
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB07012_A6Cic.seq check
>07012_07012_A6Ci_SV40pro_F_V2_F05_17.ab1
    1 GACCCGCTTT TTTGGAAGGC CTAGGCTTTT GCAAAAGCTC CCGGGAGCTT GGATATCCAT
   61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
  121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
  181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
  241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
  301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
  361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
  421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
  481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
  541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
  601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
  661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
  721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
  781 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
  841 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
  901 ACTCTGGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CCTGCCATCA CGAGATTTCG
  961 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT
 1021 GGGATGATCC TTCCAGCGCG GGGAATCTCA TGCTGGAGTT CTTCGCCCAC CCCCAACTTT
 1081 GTTTATTGCA GCTAATATGG TTAACAAATA AGCCATAGCC ATCACAAATT CACAAATAAG
1141 CATTTTTTTC ACTGGCCAAT CTCTTAGGTT TGTGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles