Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsNFKB1 (#RDB07018)

Expression vector of human NFKB1.

Alternative name	SET-0132_16
Clone info.	Expression vector of human NFKB1. Expression has not yet been confirmed.
Comment	PCR cloning, forward primer: gcagaagatgatccatatttgggaag; reverse primer: ctaaattttgccttctagaggtccttc. Nucleotide substitution T2603G (silent), G2222A (silent) to NM_003998.2.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human NFKB1 cDNA
Depositor|Developer	DNA Bank, |

 

Remarks, protocol and/or map (pdf)	RDB07018.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms

Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB07018	pCMFlag_hsNFKB1	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNFKB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07018).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08125_A5Af.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB08125_A5Afa.seq
>07018_08125_A5Af_CMV-Forward_G08_20.ab1     1 ATTTCACCTC CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGCCC GGAGGTCTAT    61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT   121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT   181 AAGGCGGCCG CAGAAGATGA TCCATATTTG GGAAGGCCTG AACAAATGTT TCATTTGGAT   241 CCTTCTTTGA CTCATACAAT ATTTAATCCA GAAGTATTTC AACCACAGAT GGCACTGCCA   301 ACAGCAGATG GCCCATACCT TCAAATATTA GAGCAACCTA AACAGAGAGG ATTTCGTTTC   361 CGTTATGTAT GTGAAGGCCC ATCCCATGGT GGACTACCTG GTGCCTCTAG TGAAAAGAAC   421 AAGAAGTCTT ACCCTCAGGT CAAAATCTGC AACTATGTGG GACCAGCAAA GGTTATTGTT   481 CAGTTGGTCA CAAATGGAAA AAATATCCAC CTGCATGCCC ACAGCCTGGT GGGAAAACAC   541 TGTGAGGATG GGATCTGCAC TGTAACTGCT GGACCCAAGG ACATGGTGGT CGGCTTCGCA   601 AACCTGGGTA TACTTCATGT GACAAAGAAA AAAGTATTTG AAACACTGGA AGCACGAATG   661 ACAGAGGCGT GTATAAGGGG CTATAATCCT GGACTCTTGG TGCACCCTGA CCTTGCCTAT   721 TTGCAAGCAG AAGGTGGAGG GGACCGGCAG CTGGGAGATC GGGAAAAAGA GCTAATCCGC   781 CAAGCAGCTC TGCAGCAGAC CAAGGAGATG GACCTCAGCG TGGTGCGGCT CATGTTTACA   841 GCTTTTCTTC CGGATAGCAC TGGCAGCTTC ACAAGGCGCC TGGAACCCGT GGTATCAGAC   901 GCCATCTATG ACAGTAAAGC CCCCCAATGC ATCCAACTTG AAAATTTGTA AGAATGGGAC   961 AGGGACAGCT GGATGTGTGA CTGGGAGGGG AGGGAAATTT ATCTTCTTTT GTGACAAAGT  1021 TCAGAAAGAT GGACATCCAG ATTTCGATTT TTATGAAAGA AGAGAAATTG ATGTAGTCTG 1081 AAAGGATTGC AGATTTTTCC CTCCACAAGA ATGGTCATAG CAACTAAAT //
Primer: BGH_rev Sequence file: RDB08125_A5Afb.seq
>07018_08125_A5Af_BGHrev_H08_23.ab1     1 GGCTGCATTT AGGTGACTAT AGAATAGGGC CCTCTAGATG CATGCTCGAG CGGCCCTAAA    61 TTTTGCCTTC TAGAGGTCCT TCCTGCCCAT AATCATGGGG CATTTTGTTG AGAGTTAGCA   121 GTGAGGCACC ACTGGTCAGA GACTCGGTAA AGCTGAGTTT GCGGAAGGAT GTCTCCACGC   181 CGCTGTCGCA GACACTGTCA CTGTCTCGGA GCTCGTCTAT TTGCTGCCTT GTGGAGGCAG   241 GCGAGAGAGG CAGCGAGTGG GCCTGAGAGG TGGTCTTCAC TGGGCTGGAG GCTGCCTGGA   301 TCACTTCAAT TGCTTCGGTG TAGCCCATTT GTCTCAGGGC CTCCACCAGC TCTCTGACTG   361 TACCCCCAGA GACCTCATAG TTGTCCATAA GTGTTTTGGA AGGAGCAGGA CTCAGCCGGA   421 AGGCATTATT AAGTATCCCC AGACCTAATT TCTGCGCCAG AGTAGCCCAG TTTTTGTCTG   481 GATCAGGAAT TTCTAGTAAC TTATACAGCT GCAGCTTCAC ATCTTCAGCC AGCTGTTTCA   541 TGTCTCCTTG TGCTAGTAAA TCATCAGATG TAAACTCTGG CTCATATGGT TTCCCATTTA   601 ATATGTCAAA TACCTGCCAG CTGGTGGCCA TATCTAGAGG CGTGGTTCCA GGCACAACTC   661 CTTCATCCTC TCCTGCATTT TCCCAAGAGT CATCCAGGTC ATAGAGAGGC TCAAAGTTCT   721 CCACCAGGGG ATCTGCTCCT GCTGCTTTGA GAAGAGCTGC CAGCCTGGTG GACCCTCTCC   781 CAGCTGCTAT ATGCAGGGGT GTGGTTCCAT CGTAGGTAGT ACTGTCCACG TGGGCATCAC   841 CCTCCAGGAG CAGGCAGCCT GCCAATGAGA TGTTGTCGTG CTCCACAGCC AGGTGCAGTG   901 CTGTGCGCCC GGACTTCTGC TCCTGAGCAT TGACGTCAGC CCCAGCGGCC CACCAGCAGC   961 AGCAAACATG GGCAGGCTAT TGCTCATCAT GGCTAGATGA ATGGCATTTC AGACCGGTCC  1021 CCGTTGGGGT GATCAGAAGT TAGTTGCCTG CTTTTTGTTG CTTTGGAGGT AGATACTGAG 1081 ACTTTAATCA TGGATCGTTC GTTGGCCAAG CA //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

Go!

Link

Other resources in our bank

• human NFKB1

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External Database
Human NFKB1

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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