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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCdx2p-luc (#RDB07098)

Reporter construct of mouse Cdx2 5' upstream.

Alternative name stock no 839
Clone info. Reporter construct of mouse Cdx2 5' upstream.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse Cdx2 genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB07098z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Cell, 123, 917-929 (2005))
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 123, 917-929 (2005))。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07098 pCdx2p-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCdx2p-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07098).

Reference section:

Niwa, H., Toyooka, Y., Shimosato, D., Strumpf, D., Takahashi, K., Yagi, R., Rossant, J., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07098_A7Ajp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F3
Sequence file: RDB07098_A7Aja.seq check
>07098_07098_A7Aj_1_f1ori_F3_A09_03_ABI24.ab1
    1 TCGGAGCTGG GCGGCGATGA GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT
   61 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT
  121 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA
  181 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT GCCATTCGCC ATTCAGGCTG
  241 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA
  301 CCATGATAAG TAAGTAATAT TAAGGTACGG GAGGTACTTG GAGCGGCCGC GAGCTCAATT
  361 AACCCTCACT AAAGGGAGTC GACTCGATCG TTGCTTCAAT TCTCCACTCT GTGCCCTGCC
  421 TACACTCTAG CCTTGGAAGA TTCCAGAGCT CCCACCGGGC TACTGATTCT TTTCCGCTTA
  481 GTGGGGGAGA AGCGGGGAAC TGGCAGCGCA AAGGCACGGG AAGACCGTGG TAGGGGCTTT
  541 CTTTCCATAG TGGTCTTTCA TCTCCCACTT CATTTGATTC ACCTGATATT TACATGTGAG
  601 CAGAGGCGAT TTTCTCTCTC TGAGAGCCTA TGGGGAAAAA AGTGTGTGTG TGTGTGTGTG
  661 TGTGTGTGTG CCCAAGTTGG AAAGGTTTGA TGGTCAGAAG CCGGTATCAC CCTTGTCCCT
  721 CCAGATGGTC AGCCCTTAGT GGTGGCCTGG AGTGCCCTGT CAAGCCATGC AATATTAAAA
  781 AATAGCAGTA TTTATTGGAC AGCCCTGAAT GTTGTGGAGC CTAGGGTGAA GACTGTATCC
  841 CATGGAGCTG ATAATACAGG GGTAGGGACG ATGCTTATAG AAATCTTCCC AATAGAACAA
  901 GACTTGGGAG CTTGCAAACC TGGGCTCTCC AGAGTCAGTC AGCTTGCTGT GGCTACTGAG
  961 AAGCAACACC ACCAGCGTGG GGGGGGGGGG GGGCATGGCC GAATTCCCCC TTCTTCTCGA
1021 AAAAAGGGAG GGAGGGGAGG GGAGGGGAAG GGAGAAGAGG AGAGCG
//
Primer: Luc_R
Sequence file: RDB07098_A7Ajb.seq check
>07098_07098_A7Aj_1_Luc_R_D09_12_ABI24.ab1
    1 CCTTTGTTTT CAGGGAACCA GGGCGTATCT CTTCATAGCC TTATGCAGTT GCTCTCCAGC
   61 GGTTCCATCT TCCAGCGGAT AGAATGGCGC CGGGCCTTTC TTTATGTTTT TGGCGTCTTC
  121 CATGGTGGCT TTACCAACAG TACCGGAATG CCAAGCTTAC TTAGATCGCA GATCTCGAGG
  181 TCGACCCAGA GCAGACCTCA CCATGCTGTC TGGGGACAAA CGTTGAAGGC TGCCAGAGGC
  241 GCGAGGCGCA GGGGGCTAGA GATAAAGGTA GCTGGCTGCA CCTCAGCCCA CGGTGCTCCG
  301 TCGGCTCCTT GCAGAGCTTC TGCCTCCCGG GCAGTCCCTC CCTCCGTCCT GCCTCCTCCC
  361 CTCTCTTCCT TCTTTCCTCC CACCTCCTTG CCAGTAAGCT GTAGAGGCGG GGATGACCCG
  421 CCGCAGGTTG GCTCGCGGAG TCCCAGGCCA GCGGCCTTAC GTGATTAACG AGTGTTTACA
  481 AGACTCTATT AGTAATGACA CAGACACCAA TGGCTGGAGA CGTCGAGGCG CAGCGCGCTA
  541 CGCACACAGT CCCCACCACC ACCACCCCCA GAAACACGAT TTGCATTTTA AAAGTTGGGG
  601 AGGGGGGCGC TCCTGAGAGG GCAGCGACCA GATCACAGCT GAATATTCAG GCCCTTCTTG
  661 CTAGCTACGA GCTTCCTCCT TCCAACCTGG GTGCACTTTA ACCTCCAATC ACAGGTTCAA
  721 AGACTGAAAT GAAGAGACTT GCAAACGCCT TGGGAAGTCC AGAAAGGCAG GCTTGGTGGG
  781 AAGCTGGGTT TGGAAACGGG CGGGCGCAGC CTTTCGTACA TTCTCAGGCA GAAAGGTGGT
841 CAAGAAAGGG GGGGGGGGGG GGGA
//
Primer: RDB07098#1
Sequence file: RDB07098_A7Ajc.seq check
>07098_07098_A7Aj_1_RDB07098#1_G09_21_ABI24.ab1
    1 GTAGTGTTAG CACTTGAGCA GGGAGACACA CATTCAATTT GTAACCTTTA CTCAGTTTAT
   61 ACTGACAGCT TTCCAAGTTG AGTCACAGAA TATCAGCTTC AACTTTTTTT TTAATGGAGA
  121 TTTTCTTTCC TTCTCCACCC CTAGAAGGTG TTTACTATGA AACAAAAGTA AATGAAGACC
  181 TTTACAGTCA GTTGGAGAAA AATCTAGAAT GGAACTTCCA AAAAAAAAAA AAAATGCATC
  241 CAAGCACGGA AGTGAACAGG TGCTTCAGAG AGAAAGCAGT ATGGAATGTG GTTAGAGCCG
  301 CAGCAAGTGG AGAACCTGAT GGATCTGGCA TTATGGTGTA TATAGGGTGG GAGTTTTGAG
  361 GCCCCAAATC ATGACAGCTG GTTTTGTAAT GGGGTTATGT GTCCAGGGTG GCACCAACAG
  421 ACACAGTAAT AATTAACGTC TGTACAGACT GCTGTGTGTG GCAAAGGCAT AGTGGCTTGT
  481 CTAATGCTAG AGACTTCCTC GGTAGCAGCG TTAGCTGTGA GGCCTTGGGA GGATCACCAG
  541 TTCTCACTGG TCTTGGTAAA ATAGTAGTGT GTGCAGACTC TGGGGCCCCC CCACCTCCCT
  601 GACTCCCCAC CACCACCACC ACCACTCTGG TAAGGCAGCC AGTCAGTGGC TACTCACTGA
  661 GACTGGCTTG TCTGTACTCC TCCCCGAACT GGCCACATAA AATTTAACCT GTAGGCTTTC
  721 CTTGGAAGAG ATTTTCATAT GACAAAACAA AGGTCATTCC AGGCCTGAGA CAATGGTGAG
  781 GGAGCTTGTG TTAGCAGAAG GCCCCGGGCA CAGGTGATGT TTGCTGCTGT GAGCCTGAGA
  841 TACTAGCTCC ACACACCACT ATTAGAGCAA AGGAGAAAAC TGAGTATCTC CAATTATTCG
  901 TTTTGCACCC AAGTGCAAAA CCAGATTTCT AAAGGGCAAA CCATGTAAAC CTTTCTCACA
  961 GCATTTTGCT TGCTTGTTGG TTTGGTTTTT TAAATCTCTT GGCTATGAAG AATAATATTT
1021 TTGGGAACAG GAGAGGAGGA T
//
Primer: pAxCALNL_F1
Sequence file: RDB07098_A7Ajd.seq check
>07098_07098_A7Aj_1_pAxCALNL_F1_H08_23_ABI24.ab1
    1 ACCAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTACAC
   61 GGCGATCTTT CCGCCCTTCT TGGCCTTTAT GAGGATCTCT CTGATTTTTC TTGCGTCGAG
  121 TTTTCCGGTA AGACCTTTCG GTACTTCGTC CACAAACACA ACTCCTCCGC GCAACTTTTT
  181 CGCGGTTGTT ACTTGACTGG CGACGTAATC CACGATCTCT TTTTCCGTCA TCGTCTTTCC
  241 GTGCTCCAAA ACAACAACGG CGGCGGGAAG TTCACCGGCG TCATCGTCGG GAAGACCTGC
  301 GACACCTGCG TCGAAGATGT TGGGGTGTTG GAGCAAGATG GATTCCAATT CAGCGGGAGC
  361 CACCTGATAG CCTTTGTACT TAATCAGAGA CTTCAGGCGG TCAACGATGA AGAAGTGTTC
  421 GTCTTCGTCC CAGTAAGCTA TGTCTCCAGA ATGTAGCCAT CCATCCTTGT CAATCAAGGC
  481 GTTGGTCGCT TCCGGATTGT TTACATAACC GGACATAATC ATAGGACCTC TCACACACAG
  541 TTCGCCTCTT TGATTAACGC CCAGCGTTTT CCCGGTATCC AGATCCACAA CCTTCGCTTC
  601 AAAAAATGGA ACAACTTTAC CGACCGCGCC CGGTTTATCA TCCCCCTCGG GTGTAATCAG
  661 AATAGCTGAT GTAGTCTCAG TGAGCCCATA TCCTTGCCTG ATACCTGGCA GATGGAACCT
  721 CTTGGCAACC GCTTCCCCGA CTTCCTTAGA GAGGGGAGCG CCACCAGAAG CAATTTCGTG
  781 TAAATTAGAT AAATCGTATT TGTCAATCAG AGTGCTTTTG GCGAAGAAGG AGAATAGGGT
  841 TGGCACCAGC AGCGCACTTT GAATCTTGTA ATCCTGAAAG CTCCTCAGAA ACAGCTCTTC
  901 TTCAAATCTA TACATTAAGA CGACTCGAAA TCCACATATC AAATATCCGA GTGTAGTAAA
  961 CATTCCAAAA CCGTGATGGA ATGGAACAAC ACTTAAAATC GCAGTATCCG GGAATGATTT
 1021 GATTGCCCAA AAATAGGATC TCTGGCATGC GAGAATCTCA CGCAGCCAGG TCTATGAGCA
 1081 GAGCGACACT TTAAGGCAGA CAGTAGATTC CAGAGAGTCC ATGATTCAGG GTGCCAATTG
1141 GTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584. [link to RRC of NBRP]