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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBRPyCAG-Klf4-IP (#RDB07102)

Expression clone of mouse Klf4 cDNA

Alternative name pCAG-Klf4-IP, pBRPyCAG-Klf4-IP, stock no 90173
Clone info. Expression clone of mouse Klf4 cDNA. cDNA fragment 632 to 2056nt of NM_010637.3 is cloned. Containing SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Comment Known difference: substitutions T to C at 980nt (Ser to Pro), G to A at 1003nt (synonym), G to A at 1449nt (Cys to Tyr), and T to C at 1999nt (synoym).
Vector backbone pBRPyCAG-cHA-IP (Plasmid)
Gene/insert name Mouse Klf4 cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB07102z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 26, 7772-7782 (2006))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07102 pBRPyCAG-Klf4-IP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBRPyCAG-Klf4-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07102).

Reference section:

Nakatake, Y., Fukui, N., Iwamatsu, Y., Masui, S., Takahashi, K., Yagi, R., Yagi, K., Miyazaki, J., Matoba, R., Ko, M.S., Niwa, H., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07102_A8Etp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB07102_A8Eta.seq check
>07102_07102_A8Et_3_pAxCA_F1_A01_01_ABI08.ab1
    1 CCATTCCGTT CTTACAATGT TTCATGCCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGAGTCT GACATGGCTG
  121 TCAGCGACGC TCTGCTCCCG TCCTTCTCCA CGTTCGCGTC CGGCCCGGCG GGAAGGGAGA
  181 AGACACTGCG TCCAGCAGGT GCCCCGACTA ACCGTTGGCG TGAGGAACTC TCTCACATGA
  241 AGCGACTTCC CCCACTTCCC GGCCGCCCCT ACGACCTGGC GGCGACGGTG GCCACAGACC
  301 TGGAGAGTGG CGGAGCTGGT GCAGCTTGCA GCAGTAACAA CCCGGCCCTC CTAGCCCGGA
  361 GGGAGACCGA GGAGTTCAAC GACCTCCTGG ACCTAGACTT TATCCTTTCC AACTCGCTAA
  421 CCCACCAGGA ATCGGTGGCC GCCACCGTGA CCACCTCGGC GCCAGCTTCA TCCTCGTCTT
  481 CCCCAGCGAG CAGCGGCCCT GCCAGCGCGC CCTCCACCTG CAGCTTCAGC TATCCGATCC
  541 GGGCCGGGGG TGACCCGGGC GTGGCTGCCA GCAACACAGG TGGAGGGCTC CTCTACAGCC
  601 GAGAATCTGC GCCACCTCCC ACGGCCCCCT TCAACCTGGC GGACATCAAT GACGTGAGCC
  661 CCTCGGGCGG CTTCGTGGCT GAGCTCCTGC GGCCGGAGTT GGACCCAGTA TACATTCCGC
  721 CACAGCAGCC TCAGCCGCCA GGTGGCGGGC TGATGGGCAA GTTTGTGCTG AAGGCGTCTC
  781 TGACCACCCC TGGCAGCGAG TACAGCAGCC CTTCGGTCAT CAGTGTTAGC AAAGGAAGCC
  841 CAGACGGCAG CCACCCCGTG GTAGTGGCGC CCTACAGCGG TGGCCCGCCG CGCATGTGCC
  901 CCAAGATTAA GCAAGAGGCG GTCCCGTCCT ACACGGTCAG CCGGTCCCTA GAGGCCCATT
  961 TGGAGCGCTG GACCCCAGCT CAGCAACGGG CCACCGGCCA ACACACACGA CTTTCCCCCT
 1021 GGGGCGGCAG CTCCCCACCA GGGACTACCC CTAACACTGA GTCCGGAGGA ACTGCTGGAC
 1081 AGCAGGACTG TCACCCTGCT GCTCTTCCCC CAGGATCATC CCATCCGGCA ACTACCTTCC
1141 TTCTGTCCAG AACTCAGAAA ATGCC
//
Primer: CSII-CMV3'
Sequence file: RDB07102_A8Etb.seq check
>07102_07102_A8Et_3_CSII-CMV3'_A02_01_ABI08.ab1
    1 GGGAAAATTC GGGAATTATC AAGCTTATCG AGCGGCCGCT TAAAAGTGCC TCTTCATGTG
   61 TAAGGCAAGG TGGTCCGACC TGGAAAAGGC CCTGTCGCAC TTCTGGCACT GAAAGGGCCG
  121 GTGCCCTGTG TGTTTGCGGT AGTGCCTGGT CAGTTCATCG GAGCGGGCGA ATTTCCACCC
  181 ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCGCCTGTG TGAGTTCGCA GGTGTGCCTT
  241 GAGATGAGAA CTCTTGGTAT AGGTTTTGCC ACAGCCTGCA TAGTCACAAG TGTGGGTGGC
  301 TGTTCTTTTC CGGGGCCACG ACCTTCTTCC CCTCTTTGGC TTGGGCTCCT CTGGCAGGCA
  361 GGAACCCGGT GGCATGAGCT CTTGATAATG GAGAGAGGGG ACTTGTGACT GCATCTGGTC
  421 TGGCAGGAAA GGAGGGTAGT TGGGCCCCGG ATGGGGATGG AATCCTGGGG GAAGAGGCAG
  481 GCCAGGGTGA CAGTCCCTGC TGTTCAGCAG TTCCTCGGGA CTCAGTGTAG GGGTAGTCCT
  541 GGTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGTGTTG GGCCGGTGGC CGTTGCTGAG
  601 CTGGGGTCCA GCGCTCAAAT GGGCCTCTAG GGACCGGCTG ACCGTGTAGG ACGGGACCGC
  661 CTCTTGCTTA ATCTTGGGGC ACATGCGCGG CGGGCCACCG CTGTAGGGCG CCACTACCAC
  721 GGGGTGGCTG CCGTCTGGGC TTCCTTTGCT AACACTGATG ACCGAAAGGG CTGCTGTACT
  781 CGCTGCCAGG GGTGGTCAGA GACGCCTTCA GCACAAACTT GCCCATCAGC CCGCCACCTG
  841 GCGGCTGAGG CTGCTGTGGC GGAATGTATA CTGGGTCCAA CTCCGGCCGC AGGAGCTCAG
  901 CCACGAAGCC GCCCGAGGGG CTCACGTCAT TGATGTCCGC CAGGTTGAAG GGGGCCCGTG
  961 GGAGGTGGCG CAGATTCTCG GCTGTAGAGG AGCCTCCACC TGTGTTGCTG GCAGCCACGC
 1021 CCGGGTCACC CCCCGGCCCG GAATCGGATA GCTGAGGCTG CAGTGGAGGG CGCGCTGGCC
 1081 AGGTCGCTGC TCGCTGGGGA GGACGAAGGA ATGAAGCTTG GCGCCGAAGG GGTGGGTTCA
1141 CGGGGTTGGC GGGGC
//
Primer: CMV_en_R
Sequence file: RDB07102_A8Etc.seq check
>07102_07102_A8Et_3_CMV_en_R_A01_01_ABI08.ab1
    1 TGGGTTTAAT GATTACTATT AATAACTAGC TAGTCAATAA TCAATGTCGA CGATGATATC
   61 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
  121 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
  181 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG
  241 TAATTTTCCT CCCGACTCTT AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA
  301 ACTGACCGCA GCTGGCCGTG CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC
  361 AGAGGGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT
  421 CAGTGTGGTT TTGCAAGAGG AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC
  481 CCAATCATTA CTATGACAAC AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGCCCCT
  541 GGCCTCCGCT TACTCTGGAG AAAAAGAAGA GAGGCTTCCA GAGGCAACTT GTCAAAACAG
  601 GACTGGCGCC TTGGAGGCGC TGTGGGGCCA CCCAAATTGA TATAATTAAG CCCCAACCGC
  661 CTCTTCCCGC CTCATTTCAG CCTCACCACT ATGTGAATAT AGCTGAATAC ACAGTTTATT
  721 GAATAAACAT TAATTTCCAG GAAATACAGT CTTTGTTTTT CCAAAGCGGT CATTGAAAAA
  781 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
  841 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
  901 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
  961 TTCGCCCCGA AGAACGTTTT CCCAATGATG AGCACTTTTT AAGTTCTGCT ATGTGGCGCG
 1021 GGTATTATCC CGTATTTGAC GCCCGGGCAA GAGCACTCGG GTCGCGGCAT AACACTAATT
 1081 CTCAGATGAC TTTGGTTGAT TACTCACAGT TCACAGAAAA GCCATCCTTA ACGGATTGGG
1141 CAATGTGAAC GAGCTAA
//
Primer: BGH_rev2
Sequence file: RDB07102_A8Etd.seq check
>07102_07102_A8Et_3_BGH_rev2_C02_01_ABI08.ab1
    1 CATCGGGGTT AAGGAAAGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GAGCTCTAGA TCATCGATGC ATGGGGTCGT GCGCTCCTTT
  121 CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC GTCAGGCACC GGGCTTGCGG GTCATGCACC
  181 AGGTGCGCGG TCCTTCGGGC ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT
  241 AGAAGGGGAG GTTGCGGGGC GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG
  301 CCGCCTCCAC TCCGGGGAGC ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG
  361 AGACGCCGAC GGTGGCCAGG AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC
  421 CTTCCATCTG TTGCTGCGCG GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA
  481 TCTCGGCGAA CACCGCCCCC GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG
  541 CGCCGTCGTC CGCGACCCAC ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT
  601 CTTGCAGCTC GGTGACCCGC TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG
  661 GGTAGTCGGC GAACGCGGCG GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG
  721 CGAGGCGCAC CGTGGGCTTG TACTCGGTCA TGGTTGTGGC CATATTATCA TCGTGTTTTT
  781 CAAAGGAAAA CCACGTCCCC GTGGTTCGGG GGGCCTAGAC GTTTTTTTTA ACCTCGACTA
  841 AACACATGTA AAGCATGTGC ACCGAGGCCC CAGATCAGAT CCCATACAAT GGGGTACCTT
  901 CTGGGGCATC CTTCAGCCCC TTGTTGAATA CGCTTGAGGA GAGCCATTTG ACTCTTTCCA
  961 CAACTATCCC AACTCACAAC GTGGCACTGG GGGTTGTGCC CGCCCTTTGC AGGTGTATCT
 1021 TTATACACGT GGGCTTTTTG GGCCGGCAGA GCAACCTGTC GCCAGGTGGG GGGGTTCGGC
 1081 TGCCTGCAAA GGGGTTCGCT ACAGACGTGA TTTGTCTCCA AGGAAAGCCT TCCAAGAAGG
1141 AACTGTGCCC T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]