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pGL4-phCOL1A1

Promoter collection, Human COL1A1 promoter

Catalog number RDB07459
Resource name pGL4-phCOL1A1
Alternative name U2774-2
Clone info. PCR amplified human COL1A1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 50202872 to 50201613(NC_000017.11); relatively -1233 to +27, where +1 corresponds to 1 nt of NM_000088.3.
Comment PCR cloning, forward primer: CAGTGGGAAGTAGCCCCTAA; reverse primer: AGGAGAAACTCCCGTCTGCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human collagen, type I, alpha 1 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human collagen, type I, alpha 1
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07459.pdf

          Distribution information

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          RDB07459 pGL4-phCOL1A1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phCOL1A1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07459).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07459_A1Htp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F (Pr0665)
          Region: pause site,insert 5'
          Sequence file: RDB07459_A1Htd.seq check
          >07459_07459_B1HbF_pGL4ins_F_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNTCCC CAGNGCAAGT GCAGGTGCCA
             61 GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG GATCAGTGGG
            121 AAGTAGCCCC TAAAACAAAG TCAATCATCC TCTGCAGCCC ATCCCACACC CCCAAAGGAA
            181 AGTTTCACCC AGACACCCAA AATATCCCAT ACATCCCCAA CACTGAGTCC AGGTACAACT
            241 GGAGAAGGGG CTTTATGCAG CTCCCAGAAA GACACCCCTT TAGCTAAGTG CCCTCCCTCC
            301 ACCCAGGTTC TCTCTGGTTT GACTGTGCTG GGAAGGAGGG TCTCTAAGCA GCCCCTGGCC
            361 ACAGCCATGG CAAACAAAAC TCTTCTCTAA GTCACCAATG ATCACAGGCC TCCCACTAAA
            421 AATACTTCCC AACTCTGGGG TGGAAGAGTT TGGGGGATGA ATTTTTANGN NNTTGCAAGC
            481 CCCAATCCCC ACCTCTGTGT CCCTAGAATC CCCCACCCCT ACCTTGGCTG CTCCATCACC
            541 CAACCACCAA AGCTTTCTTC TGCAGAGGCC ACCTAGTCAT GTTTCTCACC CTGCACCTCA
            601 GCCTCCCCAC TCCATCTCTC AATCATGCCT ANGGNTTGGA NGAANGCANT TGATTCTGTT
            661 CTGGANCACA GCANAAGAAN TGACATCCTC AAAANTAAAA CTCCCNNNNN TGNACCCCTC
          721 CNNNNNANNT CTGANNNNNN ANNNNNNNNN N
          //
          Primer: pGL4ins_R (Pr0666)
          Region: Luc 5',insert 3'
          Sequence file: RDB07459_A1Hte.seq check
          >07459_07459_B1HbF_pGL4ins_R_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNGGGT NNNNNNCGCT GGGCCCTTCT TAATGTTTTT GGCATCTTCC
             61 ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC AGATCTTGAT
            121 AGGAGAAACT CCCGTCTGCT CCGACGACTG GCCCGGGCCC CTTTTATACT GTCCTGATGG
            181 AGAGCAGGGA GGAACCCTGC CCCTCGGAGA GGGGGAGCCG GCCGCCCGTG CCCCAGCCAA
            241 TCAGAGCTGC CTGGCCCGGC CCCCAATTTG GGAGTTGGAA TGGAGAGGGG GAGGAGGAGG
          301 GAAGGGNNNN NNNNNN
          //
          Primer: SV40pA_R (Pr0882)
          Region: SV40 pA,Luc 3'
          Sequence file: RDB07459_A1Htf.seq check
          >07459_07459_B1HbF_SV40pA_R_F01_06_ABI08.ab1
              1 NNNNNNNNCN NNNATTTNNN NAATAAAGCA TTTTTTTNNC TGCATTCTAG TTGTGGTTTG
             61 TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA
            121 GAATTATTAC ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT
            181 GCGGGCGTCC AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC
            241 GCGCAGCTTC TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT
            301 CATGGTTTTA CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC
            361 GGGCAGGCCG GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG
            421 TTCGGCTGGG GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT
            481 GAAGAAGTGC TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT
            541 GTCGATGAGA GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC
            601 ACGGACGCAC AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCNAGTCCAC
            661 CACCTTANCC TCNAANANNN GNCANCANCN TNGNCNACTG CGNNAGGNTT GNCGTCCCNT
          721 TCNGGGGNGN NNNNANTNNN NNNNGNNNNN TNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl