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pCMFlag_hsCREB1, transcript variant A

Expression vector of human CREB1.

Catalog number RDB07501
Resource name pCMFlag_hsCREB1, transcript variant A
Alternative name SET-0020_8-1
Clone info. Expression vector of human CREB1. Expression has not yet been confirmed.
Comment PCR cloning, forward primer: ATGACCATGGAATCTGGAGCCGA; reverse primer: AATTAATCTGATTTGTGGCAGTAAA.
Vector backbone pCMV_S-FLAG (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CREB1,transcript variant A cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human CREB1,transcript variant A
          Reference sequence
            
           

          Distribution information

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          RDB07501 pCMFlag_hsCREB1, transcript variant A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsCREB1, transcript variant A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07501).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07501_A1L1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB07501_A1L1a.seq check
          >07501_07501_B1AjF_CMV-Forward_E10_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNTNGNN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG
            181 GCGGCCATGA CCATGGAATC TGGAGCCGAG AACCAGCAGA GTGGAGATGC AGCTGTAACA
            241 GAAGCTGAAA ACCAACAAAT GACAGTTCAA GCCCAGCCAC AGATTGCCAC ATTAGCCCAG
            301 GTATCTATGC CAGCAGCTCA TGCAACATCA TCTGCTCCCA CCGTAACTCT AGTACAGCTG
            361 CCCAATGGGC AGACAGTTCA AGTCCATGGA GTCATTCAGG CGGCCCAGCC ATCAGTTATT
            421 CAGTCTCCAC AAGTCCAAAC AGTTCAGATT TCAACTATTG CAGAAAGTGA AGATTCACAG
            481 GAGTCAGTGG ATAGTGTAAC TGATTCCCAA AAGCGAAGGG AAATTCTTTC AAGGAGGCCT
            541 TCCTACAGGA AAATTTTGAA TGACTTATCT TCTGATGCAC CAGGAGTGCC AAGGNNTGAA
            601 GAAGAGAAGT CTGAANAGGA NANTTCNGCA CCTGCCNTCA CCACTGNNAC GGTGCNNACT
            661 CCNATTTACC NAACTNGCAG TGGNNAGNAT ATNGNNNTNA CCNNNGGNNN ANNNNNNNNC
          721 NNGNNNNNNN NNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB07501_A1L1b.seq check
          >07501_07501_B1AjF_bGH_rev3_E11_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNG NNGGGCANAC AANNGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
            121 CATGCTCGAG CGGCCAATTA ATCTGATTTG TGGCAGTAAA GGTCCTTAAG TGCTTTTAGC
            181 TCCTCAATCA ATGTCTTGTT TTGATTTTCA AGCACTGCCA CTCTGTTTTC TAAACATTTC
            241 ACATATTCTT TCTTCTTTCT ACGACACTCT CGAGCTGCTT CCCTGTTCTT CATTAGACGG
            301 ACCTCTCTCT TTCGTGCTGC TTCTTCAGCA GGCTGTGTAG GAAGTGCTGG GGAGGATGCC
            361 ATAACAACTC CAGGGGCAAT AGTGCTAGTG GGTGCTGTGC GAATCTGGTA TGTTTGTACG
            421 TCTCCAGAGG CAGCTTGAAC AACAACTTGG TTGCTGGGCA CTAAGATCTG CTGTCCATCA
            481 GTGGTCTGTG CATACTGTAG AATGGTAGTA CCCGGCTGAG TGGCTGCTGC ATTGGTCATG
            541 GTTAATGTTT GCAGGCCCTG TACCCCATCG GTACCATTGT TAGCCAGCTG TATTGCTCCT
            601 CCCTGGGTAA TGGCAATATA CTGTCCACTG CTAGTTTGGT AAATTGGAGT TGGCANCGTT
            661 ACAGTGGTGA NGGCANGNGC TGNANTCTCC TCNTCNNANT NCTCNNCNNC NNNNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB07501_A1L1c.seq check
          >07501_07501_B1AjF_SV40pro_ori_F_E12_15_ABI24.ab1
              1 NNNNNNNNNC NNNGCCGCCT CGGCCTCTGA NCTATTCCNG AAGTAGTGAG GAGGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT TCGGATCTGA
            121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
            181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
            241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
            301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
            361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
            421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
            481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
            541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
            601 TCTTGTCNAT CANGANGANC TGGANNAANA NCATCNNGGG CTCGCGNCNG CCNAACTGNT
            661 CGCCNGGCTC NANGNNCNNA NGNCCNANNN NAGNNNCNCN NCNNGNNCNN NNNNNANGNN
          721 NGNNNGNNNA ANANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2024.03.26

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