RDB07534 - pGL4-phHNF4A146

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pGL4-phHNF4A146

Promoter collection, Human HNF4A promoter

Catalog number	RDB07534
Resource name	pGL4-phHNF4A146
Alternative name	U2869-7
Clone info.	PCR amplified human HNF4A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44399893 to 44401377(NC_000020.11); relatively -1.5 kb to +5, where +1 corresponds to 1 nt of NM_000457.4; relatively -1363 to +122, where +1 corresponds to 1 nt of NM_178849.2.
Comment	PCR cloning, forward primer: CCTCCAACCTGCCCTTCCAG; reverse primer: CGCATTCTCCCTGCCTCCAC. Known difference : A to C substitution at　44,400,645nt of NC_000020.11.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human hepatocyte nuclear factor 4, alpha genomic DNA
Depositor\|Developer	DNA Bank, \|

Other clones in our bank

human hepatocyte nuclear factor 4, alpha

External Database
Human hepatocyte nuclear factor 4, alpha

Reference sequence

Remarks, protocol and/or map (pdf)	RDB07534.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

Ordering
Order form [Credit Card Payment] [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]

提供条件	謝辞の表明を必要とする。付加的提供条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07534	pGL4-phHNF4A146	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHNF4A146 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07534).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Promoter Collection (Firefly Luciferase) (English text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07534_A8Eep1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081) Region: insert 5' Sequence file: RDB07534_A8Eea.seq
>07534_07534_A8Ee_pGL4-4174F_A01_01_ABI24.ab1 1 ATTGGGTCCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG 61 ATCCTCCAAC CTGCCCTTCC AGCCAGCAGT GGAGGACAGG CTCCCAGGCC TCCCAAGTCT 121 CAGGCTTTTC TTCCTCTGCC CTCACTCTCT GCCTTCTACA TCAAGACTTT ACTTCCCCAG 181 ATTGATTGGC AGCCCTTGAA AATGTCTGCA CAGAAGGCAA TGAGGGCTGG AGGGAGTGAG 241 AAGAACAGAG TGCACCATGA GCTTCAGACA GCCAGGACCA GCTGTGTAAT TTTCAGGGCC 301 CCGTCCAAAA CAAAAATGCA CAATCCCTTG TTCAAAAGTC ATGATGAATT TTAGGACAAC 361 CACAGCAGAG CACTGAACCA GGAACCAGGA CTTTCTAAGG GTTGGGTTGC CTGTGACTGC 421 ACTGGCGATA CCCCCACAAA GCCCACTCTG AAGGTAGGAG ACGGGTGGAG AGAAACAGGG 481 GGATGGCAAG GGGGATACGA AACAGGGAGA GGGAGGAGGG GGAAGAGGAT GGACGTCTAC 541 CAGGCCCCAC TTGGTGCTTG ATTTATGCCA TCTCATTTCC TTCTCAAACC ACCCTTTGAA 601 GTTGATTGTA CATTTTACAG AAAAGGAAAC TGAGGCTCGG AGAGGAGAAT CATTTACCCA 661 AGGTCCCAGT TAGTAGACGG TAGGTGCCTG AATGTAAATC CAGGTCTCTG CCTGCTCCGG 721 GAGGGGGTGG GGGTGAGGGA AACAGGAGAA TGTGATGGGA AAATCCGAGA TGGAGCCAGC 781 CTGGGCCAGA AACACTGGGA GCTGTGGGAG ACGGCGAGGG GCAGGGTGGG ATCACAGGGG 841 AGCAGAGCGG GGAATTGGGA GGGTGAATCT GGCCCTCCCA AACTTCCAGT CCATTCTGCT 901 CCCAGGGGGA AACCGGGAAA CTGCGGGGGA ACTGGGAAGG GGAGCTCCCA GAAACAAAGG 961 ATTCCAGAAA GATTGGGCAT CTGGGGGCCC TGGGATTTAG GGTTTCTAAA ATCGTGGAGC 1021 CATGGGGGCA GCCTTTATCT CTGCAAAAGC ATTGATGGTA GAAAGTCAAT GATTTGGGAA 1081 GCTAATTGGA TTAGGGGATC TTCGAGGGTA AGGGCATGTT CAGTGGCCCA //
Primer: pGL4-136R (Pr0079) Region: Luc 5', insert 3' Sequence file: RDB07534_A8Eeb.seq
>07534_07534_A8Ee_pGL4-136R_B01_04_ABI24.ab1 1 CCCTAATGGT TTTTGGGCAT CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC 61 TTGGCCGCCG AGGCCAGATC TTGATCGCAT TCTCCCTGCC TCCACGCCGC GGCCACCTGC 121 CCTACCCTGG GCGCCCACCC CGAAGGCCCC CGCCCTCCGC CCTCCCACTG CCTCCTCCCA 181 GTGCCCTCTC TGCCTTCCTT TCAAACCGTC CTCTGGGAAG ATCTGCTGGG AGTCTTGGCC 241 TAGCCTCTGT GAAGGGGTGG AGGCTCTGCC GGGGAGGGGT GGGGGTTAAT GGTTAATCGG 301 TCCCCCGCCG GTGGATAGGC TGGGCGGGGC TGCAGGGATT TGGCTGTTTG TTGGTTTCTG 361 GCTGACACCC GGGGTGCTAA TTACAACTGC TGGGGCCCTA ACTCACCGAT GTTCAGTTAT 421 CAATTGTACA AGGCAGGCAT CATGACTCAC GGGCACTCAT TTGACCCTTG ACTCACCCAC 481 CCCTCCAAGC CATTGTCACC CCAAGTCAGG CATTCTAACT GATACTATCA GGCACTGACA 541 GCCTACCTCC GAGATCCCCT AATTCAATAA CTTCCCAAAT CATTGACTTC TACCCTCAAT 601 GCTTTTGCAG AGATAAGGCT GCCCCATGGC CCACGATTTA GAAACCTAAA TCCCAGGCCC 661 CAGATGCCAA TCTTCTGGAT CCTTGTTCTG GGAGCTCCCT TCCAGTTCCC CCGCAGTTTC 721 CCGGTTCCCC TGGGAGCAGA ATGGACTGGA AGTTTGGGAG GGCCAGATTC ACCTCCAATT 781 CCCCGCTCCT GCTCCCTGTG ATCCCACCCT GCCCCTCGCC GTCTCCCACA GCTCCCAGTG 841 TTTCTGGCCC AGGCTGGCTC CATCTCGGAT TTTCCCATCA CATTCTCCTG TTTCCCTCAC 901 CCCCACCCCC TCCCGGAGCA GGCAGAGACC TGGATTTACA TTCAGGCACC TACCGTCTAC 961 TAACTGGGGA CCTTGGGTAA ATGATTCTCC TCTCCGAAGC CCTCAGTTTC CTTTTTCTGT 1021 AAAATGTACA AATCACTTTC AAGGTGGGTT TGGAGAGGGA AATGAGATTG CATTAATCAG 1081 CACAGTGGGG CCTTGTAGAC GTCCATCCGT CATACCCCCC TTCCTGCGC //
Primer: pAxCALNLF1 (Pr0166) Region: Luc 3' Sequence file: RDB07534_A8Eec.seq
>07534_07534_A8Ee_pAxCALNL_F1_C01_07_ABI24.ab1 1 TAAAGGAATC TTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA 61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA 121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT 181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC 241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG 301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG 361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT 421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG 481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA 541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT 601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA 661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC 721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT 781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG 841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT 901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA 961 ACATGCCGAA GCCGTGGGTG AAATGGCACC ACGCTGAGGG ATAGCGGGTG TCGGGGATGA 1021 TCTGGTTGCC GAAGATGGGG TCGCGGGCAT GACTGATCCG GACACAGCGT GCGGTGCGGT 1081 TAGGCCTACG CCCTTGGCAA TTCCGGTACC TGCACTACTG TTCATGATTC AGGGTCGTAA 1141 T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Suzuki, E., Transcriptional upregulation of HNF-1β by NF-κB in ovarian clear cell carcinoma modulates susceptibility to apoptosis through alteration in bcl-2 expression. Lab. Invest. 95 (8): 962-972 (2015). PMID 26030369. [link to RRC of NBRP]

2023.11.09

GNP_filter3_RDBDEP_html_231027.pl