RDB07550 - CSII-CMV-Venus-NLS

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSII-CMV-Venus-NLS (#RDB07550)

Lentivirus vector plasmid of Venus fluorescent protein with NLS driven by CMV promoter.

Clone info.	Lentivirus vector plasmid of Venus fluorescent protein with NLS driven by CMV promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSII-CMV-MCS (RDB04377) (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB07550z.seq
Remarks, protocol and/or map (pdf)	RDB07550.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07550	CSII-CMV-Venus-NLS	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-Venus-NLS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07550).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15718_A7Lmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' Sequence file: RDB15718_A7Lma.seq
>07550_15718_A7Lm_CSII-CMV5'_C01_03_ABI08.ab1 1 GTTTTCCGTG GCCTTCGGGT CTCGAGATTC TGCAGCGGCC GCGGATCCAC CATGGTGAGC 61 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 121 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 181 ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 241 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC 301 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 361 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 421 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 481 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG 541 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC 601 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC 661 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG 721 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA 781 TCTCGAGCTG ATCCAAAAAA GAAGAGAAAG GTAGATCCAA AAAAAGAAGA GAAAGGTAGA 841 TCCAAAAAAG AAGAGAAAGG TAGGATCTCT CTAGCTAGAG TTAACATCGA GGGATCAAGC 901 TTTATCGATA ATCAACCTCT GGGATTACAA AATTTTGTGA AAGATTGACT GGTATTTCTT 961 TAACTATGGT TGCTTCCCTT TTAACGCTAT GGTGGGATAC GCTGCTTTTA TGCCTTTTGT 1021 AATCATGCTA ATTGCTTTCC GTATGGCTTT TCAATTTTCT CCTTCGTTGT AATAAATTCC 1081 CTGTATGGCT GGTTCTTCAT TTAATTGAGG CAGATTGGTG TGGACTACT //
Primer: PRE_rev Sequence file: RDB15718_A7Lmb.seq
>07550_15718_A7Lm_PRE_rev_D01_04_ABI08.ab1 1 AGGTTTCAAA TAGCGTAAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGCTAGA 121 GGATCCTACC TTTCTCTTCT TTTTTGGATC TACCTTTCTC TTCTTTTTTG GATCTACCTT 181 TCTCTTCTTT TTTGGATCAG CTCGAGATCT GAGTCCGGAC TTGTACAGCT CGTCCATGCC 241 GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT 301 GGGGTCTTTG CTCAGGGCGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG 361 GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC 421 GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT 481 ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC 541 CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC 601 CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA 661 GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC 721 GAAGCACTGC AGGCCGTAGC CCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT 781 GCCGGTGGTG CAGATCAGCT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC 841 GCCGGACACG CTGAAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGGC 901 ACCACCCCCG GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGGTGGATCC GCGGCCCGCT 961 GCAGAATTTC TCGAGACCGG TTAGCGCCTA GCGGATCTGA CGGTTCACTA AACCAGGCTC 1021 TGGCTTATAT AGACCTCCCC ACCGTTACAC GGCCCTACCC GCCCCATTTG GCGTCAATGG 1081 AGCTGTAGTT GGTACGACAA TTGAAGCTC //
Primer: HIVgp_R1 Sequence file: RDB15718_A7Lmc.seq
>07550_15718_A7Lm_HIVgp_R1_E01_05_ABI08.ab1 1 CAGGCATTCT ATTCCCACTG CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT 61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA 121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG 181 GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT 241 ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT 301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT 361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT 421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC 481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT 541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC 601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG 661 TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC 721 TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC 781 TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT 841 GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC CTCCCACCGT 901 ACACGCCTAC CGCCCATTTG CGTCAATGGG GGCGGAGTTG TTACGACATT TTGGAAAGTC 961 CCGTTGATTT TGGGTGCCAA AACAAAACTT CCCAATTGAC GGTCCATGGG GTTGCAGGAC 1021 CTGGAAATCC CTCGTGAGGT CAAACCGCTA TCCAACGCCC CATGATGTAC CTGCTGAAAT 1081 GCATTCACCA TGGTAATAGC CGAATGGAAC TA //
Primer: BGH_rev2 Sequence file: RDB15718_A7Lmd.seq
>07550_15718_A7Lm_BGH_rev2_F01_06_ABI08.ab1 1 CAGGAAGGGT TAGGAAGGCA CGGGGGGAGG GGCAACAACA GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT 121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC 301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG 361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG 421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG 481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT 541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT 781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGAGGGCGAA 841 GGCGAAGACG CGGAAGAGGC CGCAGAGCCG GCAGCAGGCC GCGGGAAGGA AGGTCCGCTG 901 GATTGAGGGC CCGAAGGGAC GTAGCAGAAG GGACGTCCCG CGCAGAATCC AGGTGGCAAC 961 ACAGGGCGAG CAGCCATGGG AAAGGGACGT CAGCTTTCCC CGACACACAC GGGAGTTGTC 1021 AGTGCCCACC AGTGAGCCCC TGTCAGCAGC CGGGCCAGGC AGCGCGAATG AGTCCGTCGT 1081 GCAATAGGAA GGGGGAAGGC GAAAGTTC //
Primer: SV40pro_F Sequence file: RDB15718_A7Lme.seq
>07550_15718_A7Lm_SV40pro_F_G01_07_ABI08.ab1 1 CACTTGGTCC TTGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 841 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 901 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAAATGAGTG AGCTAACTCA 961 CATTAAATTT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGGAAACCTG TCGTGCCAGC 1021 TGCATTTAAT GAATCGGCCA ACGCCGCGGG AGGAGGCGGA TTTGCCGTAA TGAGGCGCCT 1081 CATGCGCCGT TCCCTTTCGG CATCCAG //