RDB07696 - CS-UbC-MCS-IRES2-Venus

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CS-UbC-MCS-IRES2-Venus (#RDB07696)

Backbone vector plasmid of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.

Clone info.	Backbone vector plasmid of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CS-UbC-MCS-IRES2-Venus ()
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB07696z.seq
Remarks, protocol and/or map (pdf)	RDB07696.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07696	CS-UbC-MCS-IRES2-Venus	Please contact us.	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-UbC-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07696).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17872_A9L4p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro-B_F (Pr0797) Region: UbC pro, IRES2 Sequence file: RDB17872_A9L4a.seq
>07696_17872_A9L4_1_UbCpro-B_F_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNGN GGGGCGGNGA ACGCCGATGN NTATATAAGG 61 ACGCGCCGGG TGTGGCACAG CTAGTTCCGT CGCAGCCGGG ATTTGGGTCG CGGTTCTTGT 121 TTGTGGATCG CTGTGATCGT CACTTGGTGA GTAGCGGGCT GCTGGGCTGG CCGGGGCTTT 181 CGTGGCCGCC GGGCCGCTCG GTGGGACGGA AGCGTGTGGA GAGACCGCCA AGGGCTGTAG 241 TCTGGGTCCG CGAGCAAGGT TGCCCTGAAC TGGGGGTTGG GGGGAGCGCA GCAAAATGGC 301 GGCTGTTCCC GAGTCTTGAA TGGAAGACGC TTGTGAGGCG GGCTGTGAGG TCGTTGAAAC 361 AAGGTGGGGG GCATGGTGGG CGGCAAGAAC CCAAGGTCTT GAGGCCTTCG CTAATGCGGG 421 AAAGCTCTTA TTCGGGTGAG ATGGGCTGGG GCACCATCTG GGGACCCTGA CGTGAAGTTT 481 GTCACTGACT GGAGAACTCG GTTTGTCGTC TGTTGCGGGG GCGGCAGTTA TGGCGGTGCC 541 GTTGGGCAGT GCACCCGTAC CTTTGGGAGC GCGCGCCCTC GTCGTGTCGT GACGTCACCC 601 GTTCTGTTGG CTTATAATGC AGGGTGGGGC CACCTGCCGG TAGGTGTGCG GTAGGCTTTT 661 CTCCGTCGCA GGACGCAGGG TTCGGGCCTA GGGTAGGCTC TCCTGAATCG ACAGGCGCCG 721 GACCTCTGGT GAGGGGANGG ATAAGTGANG CGTCAGTTTC TTTGGTCGGT TTTATGTACC 781 TATCTTCTTA AGTAGCTGAA GCTCCGGTTT TGAACTATNC GCTCGNGNTT GGCGAGTGTG 841 TTTTGTGAAG TTTTTTTANG CNNNNTTTGA AATGTANTCA TNTGGGNNNA TATGTAATTT 901 TTCAGTGTTN NANTANTAAA TTGNCCGCTA AATTCNNGNN NNNTTTTTGG CTTTTTTTNN 961 NTNNNNNNNN NN //
Primer: PRE_rev (Pr0250) Region: PRE, Venus 3' Sequence file: RDB17872_A9L4b.seq
>07696_17872_A9L4_1_PRE_rev_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNA ANGGNNCNAC NTAGTTAAGA NTACCAGTCA ATCTTTCNCA 61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG 121 GCTCGAGAGG CCTTGAATTC TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG 181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG 241 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG 301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC 361 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG 421 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC 481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG 541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG 601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG 661 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC 721 AGCTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGNAC 781 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGNGAACAGC 841 TCCTCGCCNN TGCTCACNAT GGNTGTGGNC ATATTATCAT CGNGNTTTTT CAAANGAAAA 901 CCACGNNCNN NNNGNTCNGG GGGGGCCNAN NNNNTTTTTT NANNTNNANT AAANNCNTNN 961 NANNNATNNG NNNNNNNNGN NNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, U5, HIV-1 psi Sequence file: RDB17872_A9L4d.seq
>07696_17872_A9L4_1_CMV_Forward_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNTG NNNCNNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG 121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT 181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT 241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA 301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG 361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG 421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC 481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG 541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA 601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA 661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCNAGGAAG 721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG 781 CTGATCTTCA GACCTGGANG NNGANATATG ANGGNNAATT GGAGAAGTGA ATTATATAAA 841 TATAAAGTAG TAAAAATTGA ANCNTTNNGA NTAGCNCCNN CCNAGGCNAA NANAANNNNN 901 NNNCANNNAN AAAAAAAGAN CNNNNGGNAN TANGNNCTTN NNNNNNNNGN NTNNNTNNGN 961 NNCNNCNNNA NNNNNNNNNG GNNNN //
Primer: RRE-M_R (Pr0651) Region: RRE, HIV-1 psi, U5 Sequence file: RDB17872_A9L4c.seq
>07696_17872_A9L4_1_RRE-M_R_B03_06_ABI24.ab1 1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CNATAGCCCT CAGCANATTG TTCTGCTGCT 61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA 121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT 181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT 241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG 301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT 361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG 421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT 481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG 541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC 601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG 661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC 721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG 781 AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT 841 TCCACACTGA CTAAAAGGGT CTGANGGATC TCTAGTTACC AGANNCACAC AACAGANGGG 901 CNCACACTAC TNGANNCANT CNAGGNAAGC TTTATTGAGG NTTAANCAGN GGNNTNCNNA 961 NNNAGCCNGA NNNNNNCNAN NNNCNNNNNT GGNNNNNNNA GANANNNCCA NNANNNNNNA 1021 ANN //
Primer: cPPT_CTS-E_R (Pr0725) Region: cPPT_CTS Sequence file: RDB17872_A9L4e.seq
>07696_17872_A9L4_1_cPPT_CTS-E_R_D02_04_ABI08.ab1 1 NNNNNNNNNN NNTTTNNNNN NNTCTTTAGT TTGNNNGTCT GTTGCTATTA TGTCTACTAT 61 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC 121 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT 181 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG 241 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC 301 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG 361 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCNATAAT TCTTGTTCAT 421 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAANGANTG TATTAAGCTT GTGTAATTGT 481 TNATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGANTNCN AATCTGNTNC ANANATTTAT 541 TACTNCNNCT ANNNNTCCNA GGNACANCAN NGNNGNNANT GANTTTTCCA NANNNNCNNN 601 ANNNCCNNNN NNNNNN //
Primer: IRES-F (Pr0036) Region: IRES2, Venus 5' Sequence file: RDB17872_A9L4f.seq
>07696_17872_A9L4_1_IRES-F_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTCNNNNGG NNNNNNNNNG TCTAGGCCCC CCGAACCNCG 61 GGGNNGTGGT TTTCCTTTGN AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG 121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 241 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 301 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 541 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC 601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG 661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGANCTAC 721 CAGTCCGCCC TGAGCAAAGA NCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC 781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGANCTGT ACAAGTAAGA ATTCNAGGCC 841 TCTCGAGCCT CTAGAGTTAA CATCGAGGGA TCAAGCTNAT CGATAATCAA CCTCNNGATT 901 ACNAAATTTG TGAAAGANTG NNNGGTANTN NNNNNNANNN NGNNNCNTTT ACGCNATGNG 961 GNNNNNCNNN NNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), del U3 Sequence file: RDB17872_A9L4g.seq
>07696_17872_A9L4_1_BGH_rev2_F02_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGN GGNNGGGCAA NCANCAGNNG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA 121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG 181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC 241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC 301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC 361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC 421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA 481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA 541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC 601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG 661 TACCTGANGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA 721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG 781 ACGGTATCGA TGCGGGGANG CGGCCCNAAG GGANATCCGA NTCGTCTGAN GGCGAANGCG 841 AANANNCGNA ANANGCCGCA NANCCGGCAG CNNNNCNCGG GNANGNANNN TNCGCNNGNN 901 TNANGGNCGA ANGGANGNAN CAGAANNNAN NTCNNNCGCA NNNNCCNNNN GGNNNNNCNN 961 NNNANNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, Zeo Sequence file: RDB17872_A9L4h.seq
>07696_17872_A9L4_1_SV40pro_ori_F_G02_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTANGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC 181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC 241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG 301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG 361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG 421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG 481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC 541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC 601 GCCGCCTTCT ATGAAAGGNT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC 661 CTCCAGCGCG GGGNTCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT 721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA 781 CTGCATTCTN NNTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG 841 NCNANNNCTA NCTAGAGCTN GNCGNAATCN NGGNCATANC TGTTTCCTGN NNNAAANTGN 901 TATCCGCNCA NAATTCCACA CNANNTACNA NNNNGNANCN TAAANNNNNA NGCCNNGGNN 961 NNNNNNNN //