Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-UbC-MCS-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17872_A9L4p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro-B_F (Pr0797) Region: UbC pro, IRES2 Sequence file: RDB17872_A9L4a.seq
>07696_17872_A9L4_1_UbCpro-B_F_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNGN GGGGCGGNGA ACGCCGATGN NTATATAAGG    61 ACGCGCCGGG TGTGGCACAG CTAGTTCCGT CGCAGCCGGG ATTTGGGTCG CGGTTCTTGT   121 TTGTGGATCG CTGTGATCGT CACTTGGTGA GTAGCGGGCT GCTGGGCTGG CCGGGGCTTT   181 CGTGGCCGCC GGGCCGCTCG GTGGGACGGA AGCGTGTGGA GAGACCGCCA AGGGCTGTAG   241 TCTGGGTCCG CGAGCAAGGT TGCCCTGAAC TGGGGGTTGG GGGGAGCGCA GCAAAATGGC   301 GGCTGTTCCC GAGTCTTGAA TGGAAGACGC TTGTGAGGCG GGCTGTGAGG TCGTTGAAAC   361 AAGGTGGGGG GCATGGTGGG CGGCAAGAAC CCAAGGTCTT GAGGCCTTCG CTAATGCGGG   421 AAAGCTCTTA TTCGGGTGAG ATGGGCTGGG GCACCATCTG GGGACCCTGA CGTGAAGTTT   481 GTCACTGACT GGAGAACTCG GTTTGTCGTC TGTTGCGGGG GCGGCAGTTA TGGCGGTGCC   541 GTTGGGCAGT GCACCCGTAC CTTTGGGAGC GCGCGCCCTC GTCGTGTCGT GACGTCACCC   601 GTTCTGTTGG CTTATAATGC AGGGTGGGGC CACCTGCCGG TAGGTGTGCG GTAGGCTTTT   661 CTCCGTCGCA GGACGCAGGG TTCGGGCCTA GGGTAGGCTC TCCTGAATCG ACAGGCGCCG   721 GACCTCTGGT GAGGGGANGG ATAAGTGANG CGTCAGTTTC TTTGGTCGGT TTTATGTACC   781 TATCTTCTTA AGTAGCTGAA GCTCCGGTTT TGAACTATNC GCTCGNGNTT GGCGAGTGTG   841 TTTTGTGAAG TTTTTTTANG CNNNNTTTGA AATGTANTCA TNTGGGNNNA TATGTAATTT   901 TTCAGTGTTN NANTANTAAA TTGNCCGCTA AATTCNNGNN NNNTTTTTGG CTTTTTTTNN 961 NTNNNNNNNN NN //
Primer: PRE_rev (Pr0250) Region: PRE, Venus 3' Sequence file: RDB17872_A9L4b.seq
>07696_17872_A9L4_1_PRE_rev_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNA ANGGNNCNAC NTAGTTAAGA NTACCAGTCA ATCTTTCNCA    61 AATTTTGTAA TCCAGAGGTT GNTTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG   121 GCTCGAGAGG CCTTGAATTC TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG   181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG   241 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG   301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC   361 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG   421 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC   481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG   541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG   601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG   661 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC   721 AGCTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGNAC   781 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGNGAACAGC   841 TCCTCGCCNN TGCTCACNAT GGNTGTGGNC ATATTATCAT CGNGNTTTTT CAAANGAAAA   901 CCACGNNCNN NNNGNTCNGG GGGGGCCNAN NNNNTTTTTT NANNTNNANT AAANNCNTNN 961 NANNNATNNG NNNNNNNNGN NNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, U5, HIV-1 psi Sequence file: RDB17872_A9L4d.seq
>07696_17872_A9L4_1_CMV_Forward_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTG NNNCNNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG   121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT   181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT   241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA   301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG   361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG   421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC   481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG   541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA   601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA   661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCNAGGAAG   721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG   781 CTGATCTTCA GACCTGGANG NNGANATATG ANGGNNAATT GGAGAAGTGA ATTATATAAA   841 TATAAAGTAG TAAAAATTGA ANCNTTNNGA NTAGCNCCNN CCNAGGCNAA NANAANNNNN   901 NNNCANNNAN AAAAAAAGAN CNNNNGGNAN TANGNNCTTN NNNNNNNNGN NTNNNTNNGN 961 NNCNNCNNNA NNNNNNNNNG GNNNN //
Primer: RRE-M_R (Pr0651) Region: RRE, HIV-1 psi, U5 Sequence file: RDB17872_A9L4c.seq
>07696_17872_A9L4_1_RRE-M_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CNATAGCCCT CAGCANATTG TTCTGCTGCT    61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA   121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT   181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT   241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG   301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT   361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG   421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT   481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG   541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC   601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG   661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC   721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG   781 AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT   841 TCCACACTGA CTAAAAGGGT CTGANGGATC TCTAGTTACC AGANNCACAC AACAGANGGG   901 CNCACACTAC TNGANNCANT CNAGGNAAGC TTTATTGAGG NTTAANCAGN GGNNTNCNNA   961 NNNAGCCNGA NNNNNNCNAN NNNCNNNNNT GGNNNNNNNA GANANNNCCA NNANNNNNNA 1021 ANN //
Primer: cPPT_CTS-E_R (Pr0725) Region: cPPT_CTS Sequence file: RDB17872_A9L4e.seq
>07696_17872_A9L4_1_cPPT_CTS-E_R_D02_04_ABI08.ab1     1 NNNNNNNNNN NNTTTNNNNN NNTCTTTAGT TTGNNNGTCT GTTGCTATTA TGTCTACTAT    61 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC   121 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT   181 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG   241 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC   301 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG   361 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCNATAAT TCTTGTTCAT   421 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAANGANTG TATTAAGCTT GTGTAATTGT   481 TNATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGANTNCN AATCTGNTNC ANANATTTAT   541 TACTNCNNCT ANNNNTCCNA GGNACANCAN NGNNGNNANT GANTTTTCCA NANNNNCNNN 601 ANNNCCNNNN NNNNNN //
Primer: IRES-F (Pr0036) Region: IRES2, Venus 5' Sequence file: RDB17872_A9L4f.seq
>07696_17872_A9L4_1_IRES-F_E02_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTCNNNNGG NNNNNNNNNG TCTAGGCCCC CCGAACCNCG    61 GGGNNGTGGT TTTCCTTTGN AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG   121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC   181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC   241 CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC   301 CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC   421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC   481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC   541 AACTACAACA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC   601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG   661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGANCTAC   721 CAGTCCGCCC TGAGCAAAGA NCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC   781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGANCTGT ACAAGTAAGA ATTCNAGGCC   841 TCTCGAGCCT CTAGAGTTAA CATCGAGGGA TCAAGCTNAT CGATAATCAA CCTCNNGATT   901 ACNAAATTTG TGAAAGANTG NNNGGTANTN NNNNNNANNN NGNNNCNTTT ACGCNATGNG 961 GNNNNNCNNN NNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), del U3 Sequence file: RDB17872_A9L4g.seq
>07696_17872_A9L4_1_BGH_rev2_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGN GGNNGGGCAA NCANCAGNNG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGANGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG   781 ACGGTATCGA TGCGGGGANG CGGCCCNAAG GGANATCCGA NTCGTCTGAN GGCGAANGCG   841 AANANNCGNA ANANGCCGCA NANCCGGCAG CNNNNCNCGG GNANGNANNN TNCGCNNGNN   901 TNANGGNCGA ANGGANGNAN CAGAANNNAN NTCNNNCGCA NNNNCCNNNN GGNNNNNCNN 961 NNNANNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, Zeo Sequence file: RDB17872_A9L4h.seq
>07696_17872_A9L4_1_SV40pro_ori_F_G02_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTANGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC   241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG   301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG   361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG   421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG   481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC   541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC   601 GCCGCCTTCT ATGAAAGGNT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC   661 CTCCAGCGCG GGGNTCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT   721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA   781 CTGCATTCTN NNTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG   841 NCNANNNCTA NCTAGAGCTN GNCGNAATCN NGGNCATANC TGTTTCCTGN NNNAAANTGN   901 TATCCGCNCA NAATTCCACA CNANNTACNA NNNNGNANCN TAAANNNNNA NGCCNNGGNN 961 NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl