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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL3-AID R1 del.-04

Reporter construct of mouse Aicda locus, position -839 to +101.

Catalog number RDB07827
Resource name pGL3-AID R1 del.-04
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -839 to +101.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 1a.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
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External Database
Mouse Aicda

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 学術機関の学術研究に限る。 営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          RDB07827 pGL3-AID R1 del.-04 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07827_A4Gi.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07827_A4Gia.seq check
          >07827_07827_A4Gi_pGL4-4174F_D11_11.ab1
              1 AGGAGGGGTC CAGAAATTTC TCTATCGATA GGTACCGAGC TCTTACGCGT GCTAGCCCGG
             61 GCTCGAGTGA TCAGGGTTCA GATCCCAGCA AGCACACATG GTGGCTTTCA ACTGCCTGCT
            121 GGTCCTCTGC AATAGAAGTA AGTGCTCTTA ATCACTGGGC CAGCTCTCCA GGCCTCCAGT
            181 AAGGTATTTT TAATGAGGAA AAGGAGTTCT TTTTTTTTTT AATACTTTTT GACACACACA
            241 CACACACAAA ATTAAAATAA ATCACTTTTT GGTGCAAGCA ACTAGTCTTT CTAGCTATCT
            301 TATAATGTCA TTTTAAAAAA AGAAAAATAT ATTAGAGAAT TGGGAGGCTA AAGTTCACTC
            361 TCTGGATGCT GTGGTGGTCA ACCCCCATCT CTACTGAGGC ATAAAACTGA GTGTAACAAA
            421 CGGAAGGAAC AGATACTGTA AGTTCAAGAA GCACAAGATG CAACTGAAGT GCCATTTAAG
            481 GCCACTTTAA GTCACTATGA CTGCTATCAT TCTTGTTATC ATAGTTTTAA AATTAGGAAG
            541 TATGCACAGA CCTTAGGTGT GATACCCGGG ACCACACACA CACACACACA CACACACTCA
            601 CAGAGCTCAT TATCATGATA CCAATGTGAG AAGTGTCCAG TGCTATTGTC TCCTGATCTT
            661 TGTTACCTGT GATACCTGGG CTGGCTTTTT AGAGGAACAG CCTCGAAGGA AGTTGGACAT
            721 TAAGCATGAG CAGAACTGGC CCCCCCCCCC AATCATTTAA TCCGTGTGGC TTTGCCCACC
            781 ACAGCCCCGC CCATCTTTAC TGGACCCAAC CCAGGAGGCA GATGTTGGAT ACCTGGTGGT
            841 AGTGATGCTG TCGTGGGGGA GGAGCCCACA AGAGCAAGCT CAGATTTGAA TGCCAGGGGC
            901 CAGTGCTCTG TCACACAACA GCACTGAAGC AGCCTTGCTT GAAGCAAGCT TCCTTTGGCC
            961 TAAGACTTTG AGGGAGTCAA GAAAGTCACG CTGGGAGACC GATCCATGGA AGACGCCCAA
           1021 AAACATAAAG AAAGGCCCGG GCGCCAATTC TATCCGCTGA GATGGGAAAC CGCTGGGAGA
           1081 GCACTGCATA AGCTTATGAG AGAATACGCC CTGGATCTGA CCATTGCTTT ACAGATGCAC
          1141 AATAATTCGA AGGGTGGCA
          //
          Primer: Luc_R
          Sequence file: RDB07827_A4Gib.seq check
          >07827_07827_A4Gi_Luc_R_E11_14.ab1
              1 CATTGCTTCA GGGACCAGGG CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT
             61 TCCATCTTCC AGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
            121 GGATCGGTCT CCAGCGTGAC TTTCTTGACT CCCTCAAAGT CTTAGGCCAA AGGAAGCTTG
            181 CTTCAAGCAA GGCTGCTTCA GTGCTGTTGT GTGACAGAGC ACTGGCCCCT GGCATTCAAA
            241 TCTGAGCTTG CTCTTGTGGG CTCCTCCCCC ACGACAGCAT CACTACCACC AGGTATCCAA
            301 CATCTGCCTC CTGGGTTGGG TCCAGTAAAG ATGGGCGGGG CTGTGGTGGG CAAAGCCACA
            361 CGGATTAAAT GATTGGGGGG GGGGGCCAGT TCTGCTCATG CTTAATGTCC AACTTCCTTC
            421 GAGGCTGTTC CTCTAAAAAG CCAGCCCAGG TATCACAGGT AACAAAGATC AGGAGACAAT
            481 AGCACTGGAC ACTTCTCACA TTGGTATCAT GATAATGAGC TCTGTGAGTG TGTGTGTGTG
            541 TGTGTGTGTG TGGTCCCGGG TATCACACCT AAGGTCTGTG CATACTTCCT AATTTTAAAA
            601 CTATGATAAC AAGAATGATA GCAGTCATAG TGACTTAAAG TGGCCTTAAA TGGCACTTCA
            661 GTTGCATCTT GTGCTTCTTG AACTTACAGT ATCTGTTCCT TCCGTTTGTT ACACTCAGTT
            721 TTATGCCTCA GTAGAGATGG GGGTTGACCA CCACAGCATC CAGAGAGTGA ACTTTAGCCT
            781 CCCAATTCTC TAATATATTT TTCTTTTTTT AAAATGACAT TATAAGATAG CTAGAAAGAC
            841 TAGTTGCTTG CACCAAAAAG TGATTTATTT TAATTTTGTG TGTGTGTGTG TGTCAAAAAG
            901 TATTAAAAAA AAAAAGAACT CCTTTTCCTC ATTAAAAATA CCTTACTGGG AGGGCCTGGA
            961 GAGCTGGCCC AGTGATTAAA GAGCACTTAC TTCTATTGCA GAGGACCAGC AGGCAGTTGA
           1021 AGCCACCATG GTGGTGCTTT GCTGGGATCT GACCCCTGAT CACTCGGAGG CCCGGACTAG
           1081 CACGGCGTAG GAGCTCGGCT ACTATCGATT AGAGAAATTG ATCCTGGGCA CCTGGCAACG
          1141 TTGCACCTGG GGGGACAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl