RDB07880 - pGL3-AID R1+R4 full

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pGL3-AID R1+R4 full (#RDB07880)

Reporter construct of mouse Aicda locus, position -9226 to -7585 plus -1527 to +101.

Clone info.	Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585 plus -1527 to +101.
Comment	Nat. Immunol. (2010) 2, 148-154, Fig. 1b.
Vector backbone	pGL3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Aicda genomic DNA
Depositor\|Developer	Honjo, Tasuku \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07880	pGL3-AID R1+R4 full	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R1+R4 full was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07880).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07880_A3Af.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3_RV3 Sequence file: RDB07880_A3Afa.seq
>7880_7880_A3Af_pGL3-Rv3_E02_14.ab1 1 GGTTAGGGGC AGACATTTCT CTATCGATAG GTACCCACCA CTCTCGGCTG TTCTGGAACT 61 CTCTGTAACC TCGGGCTGTA TGAGTGTATG TGAGTGTGTG TATATGGGTG TGGGGGGGGT 121 GAGAGTATAT GAGTGTATGT GAGTGTGTAT GTATGGGTGT GAGGTGTGTG TGTGTATGAG 181 TGTGTGTATG AGTGTGTGTA TGAGTGTGTG TGAGTGTGTG TATATGGGGG GGTGACCCCC 241 GATGCTTTCC AGAGACCGCC AGCCCCTCAT GCTGGCCATA ACGAAACCTC ACCTGTGTGT 301 CTCATAGGGA AAGCGAGGTG GTGAACTCCA GATGTCCAAA ACTCAGCTCA GCCGCAGGCG 361 ACTCGATTTC CAAGTCACAG TATTTTTGGT TAAAAACAAT GTGACCAAGT TGGCTTGTGG 421 TTTTTCAATA GAAGTTAATG AGGGGAAAGA GAAGTTAATG AAGAAAATAA AAGTTGATCA 481 AAACCCTGTA AACTGCTTTG CCGCTGTACC AGTTCCAAAT TTCCCGGGAC TGGAAAATGG 541 TTTTTGTAAC TAAGTGAGAT CTCTTTCCTT CCTTTAGAAG AAGGCAAATG ATAGAATTTG 601 GCTTTCTTAC AAATCAAGGG TTTTGTATTT GTTCCTATGG CATGTGTACG GCATCTGTAA 661 GAGAGGGTGT GGGCGTGCGT GTGCGTGTGC TACAGCTCGA GGTGTCTCCA GTATGTCATT 721 CCCTGGACTA TTGGTCCAAA GTAGGGCAAA GGTCCGAGCC CTCATCCTGT GAGAACAAGT 781 TAAAAGGGAC TTGTCCCACC CCACCACCCT GGGGGTTCCC CTTAACCCTG TAATGTCCAC 841 GGGCTGGGTG CTGGGTTTCT TCCCGGTGCC CTGAAATTTT TTATTCCAAG AATTCATTAA 901 TTCACTTA //
Primer: pGL3_RV6R Sequence file: RDB07880_A3Afb.seq
>7880_7880_A3Af_pGL3-Rv6R_F02_17.ab1 1 GTATCCGTCA GCGGATAGAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG 61 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTGCT 121 TCAAGCAAGG CTGCTTCAGT GCTGTTGTGT GACAGAGCAC TGGCCCCTGG CATTCAAATC 181 TGAGCTTGCT CTTGTGGGCT CCTCCCCCAC GACAGCATCA CTACCACCAG GTATCCAACA 241 TCTGCCTCCT GGGTTGGGTC CAGTAAAGAT GGGCGGGGCT GTGGTGGGCA AAGCCACACG 301 GATTAAATGA TTGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA 361 GGCTGTTCCT CTAAAAAGCC AGCCCAGGTA TCACAGGTAA CAAAGATCAG GAGACAATAG 421 CACTGGACAC TTCTCACATT GGTATCATGA TAATGAGCTC TGTGAGTGTG TGTGTGTGTG 481 TGTGTGTGTG GTCCCGGGTA TCACACCTAA GGTCTGTGCA TACTTCCTAA TTTTAAAACT 541 ATGATAACAA GAATGATAGC AGTCATAGTG ACTTAAAGTG GCCTTAAATG GCACTTCAGT 601 TGCATCTTGT GCTTCTTGAA CTTACAGTAT CTGTTCCTTC CGTTTGTTAC ACTCAGTTTT 661 ATGCCTCAGT AGAGATGGGG GTTGACCACC ACAGCATCCA GAGAGTGAAC TTTAGCCTCC 721 CAATTCTCTA ATATATTTTT CTTTTTTTTA AAATGACATT ATAAGATAGC TAGAAAGACT 781 AGTTGCTTGC ACCAAAAAGT GATTTATTTT AATTTTGTGT GTGTGTGTGT GTCAAAAGTA 841 TTAAAAAAAA AAAAGAACTC CTTTTCCTCA TTAAAAATAC CTTACTGGGA GGCCTGGAGA 901 GCTGGCCCAG TGATTTAGAG CACTTTACTT CTTATTGGCA GAGACAGCAG CAGTGAAGGC 961 ACAATGTGTG CTGCTGGATC TGACCTTGAT CATCTGAGAA CATCGTTGGC TTCCTTCCTT 1021 CTCGTCGTCG TACTACTACT ACTCATACAT ACATCTCTTA CCCGCAGTGC TA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

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mouse Aicda

External Database
Mouse Aicda

Reference sequence