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CSII-CMV-MCS-IRES2-mRFP1

Backbone vector plasmid of lentivirus construct driven by CMV promoter with mRFP1 protein marker.

Catalog number RDB07918
Resource name CSII-CMV-MCS-IRES2-mRFP1
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with mRFP1 protein marker.
Comment XbaI site at 4490nt is not exist. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence RDB07918z.seq
            Remarks, protocol and/or map (pdf) RDB07918.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07918 CSII-CMV-MCS-IRES2-mRFP1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-CMV-MCS-IRES2-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07918).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17905_B0Aep1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: cPPT+CTS_F (Pr0161)
            Region: CMV en 2,CMV pro 2,IRES2
            Sequence file: RDB17905_B0Aea.seq check
            >07918_17905_B0Ae_1_cPPT+CTS_F_A11_02_ABI24.ab1
                1 NNNNNNNNNN NNNTNGNNNA NTAGTANTCN ATTACGGGGT CATTAGTTCN TAGCCCATAT
               61 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
              121 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
              181 CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
              241 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
              301 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
              361 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
              421 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
              481 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
              541 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTG GTTTAGTGAA CCGTCAGATC
              601 CGCTAGCGCT ACCGGTCTCG AGAATTCTGC AGCGGCCGCG GATCCGCCCC TCTCCCTCCC
              661 CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA
              721 TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG
              781 TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CNNCTCTCGC CNAAGGAATG CAAGGTCTGT
              841 TGAATGTCGT GAANGAAGCA GTTCCTCTGG AAGCTTCTTG AAGANAAACA ACGTCTGTAN
              901 CGANCCTTTG CAGGCAGCGG AANCCCCCAC CNNGNCGANA GGNGCCNNNG CGGNCNAAAG
              961 CCNCGNGNAT NANANACNCC TGCAAANGNG NNACNNCCCN NNNNNCNNNN NGTGANTNNG
            1021 NNANNNGNGN NNNANTNNAN NNNNNNNNN
            //
            Primer: IRES-F (Pr0036)
            Region: IRES2,mRFP1
            Sequence file: RDB17905_B0Aeb.seq check
            >07918_17905_B0Ae_1_IRES-F_E11_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTNN GTCGANGTNN NNNNNNCGTC TAGGCCCCCC GAACCACGGG
               61 GNCGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACGGGCCTAT GGCCTCCTCC
              121 GAGGACGTCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC CGTGAACGGC
              181 CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC
              241 AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG
              301 TTCCAGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG
              361 CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG
              421 GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG
              481 CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG
              541 GCCTCCACCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGATGAGG
              601 CTGAAGCTGA AGGACGGCGG CCACTACGAC GCCGAGGTCA AGACCACCTA CATGGCCAAG
              661 AAGCCCGTGC AGCTGCCCGG CGCCTACAAG ACCGACATCA AGCTGGACAT CACCTCCCAC
              721 AACGAGGACT ACACCATCGT GGAACAGTAC GAGCGCGCCG ANGGCCGCCA CTCCACCGGC
              781 GCCTAAGCGG NCGCACTCGA CTAGAGTTAA CATCGAGGGA TCAAGCTTAT CGATAATCNA
              841 CCTCTGGATT ACNAAATTTG TGAAAGANTG ACTGGTATTC TTAACTATGT TGCTCCTTTT
              901 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CNGTATGGNT
              961 TTCNTTTTCT CCNNCTNGNA TAAATCCNNN NTNNNTGNCT CTTTNTGAGG ANNNNNNGNN
            1021 CNNNNGNCNN NNNNCGNNNN NNNNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE,mRFP1
            Sequence file: RDB17905_B0Aec.seq check
            >05552_18578_B0Kq_PRE_rev_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCNNCNTA GTTAAGAATA CCAGTCAATC TTTCACAAAT
               61 TTTGTAATCC AGAGGTTGNN TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT
              121 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG
              181 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG
              241 GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG
              301 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG GCGGATCTTG
              361 AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT GTGGCTGTTG
              421 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC
              481 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG
              541 TTGCCGTCGT CCTTGAAGAA GANGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC
              601 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG CANGCCGTAN
              661 CCNANGGTGG TCACGANGGT GGGNCAGGGN NCGGGNAGCT NGNCNNNNGN GNANNNNNNN
            721 NNN
            //
            Primer: HIV-B_R (Pr0673)
            Region: HIV-1 psi,5' LTR,CMV pro 1,CMV en 1
            Sequence file: RDB17905_B0Aed.seq check
            >07918_17905_B0Ae_1_HIV-B_R_C11_08_ABI24.ab1
                1 NNNNNNNNNN NNNNCNNNNN AANNTTTTGG CGTACTCNCC AGTCGCCGCC CCTCGCCTCT
               61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
              121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
              181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
              241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
              301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
              361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
              421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
              481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
              541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
              601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
              661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
              721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT
              781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
              841 TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT GANCCCGTAA TTGATTACTA
              901 TTAATAACTA GTCAATAATC AATGTCAACG CGNATATCTG GNCCGTANNT CGCGAANCAN
              961 CGNAAAANNC CTAACCCTAA NCANANTCTT CNTGNNATTG NCGGNNNNGC CTNNNCNNNN
            1021 NNGNANCTNN AATTTNGNNC NNNNNNNNNN
            //
            Primer: cPPT/CTS-E_R (Pr0725)
            Region: cPPT_CTS,RRE
            Sequence file: RDB17905_B0Aee.seq check
            >07918_17905_B0Ae_1_cPPT_CTS-E_R_D11_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTTG NATTCTTNAG TTTGTATGTC TGTTGCTATT ATGTCTACTA
               61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
              121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
              181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
              241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
              301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
              361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
              421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
              481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
              541 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC
              601 CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG
              661 AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC
              721 AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC
              781 GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCA AGAACCCAAG GAACAAAGCT
              841 CCTATTCCCA CTGCTCTTTT TTCTCTCTGC ACCACTCTTC TCTTTGCNNT GGTGGGTGCT
              901 ACTCCNAATG GNTCNATTTT NACNANTTNA TATTTATATA ATTCACTTCT NNNATTGNCC
              961 NNCANATCTC CTNCNNCNNG NCTNAANNNC NNCGGNCGGN NGCTTGNTGN GNNNNGNNNN
            1021 TANNTTTGNT TNNNNNNNNN N
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGHpA,3' LTR,PRE
            Sequence file: RDB17905_B0Aef.seq check
            >07918_17905_B0Ae_1_BGH_rev2_F11_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GNAACAACAG ATGGCTGGCA ACTAGAAGGC
               61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
              121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
              181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
              241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
              301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
              361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
              421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
              481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
              541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
              601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
              661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
              721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
              781 TCGACGGTAT CGATGCGGGG ANGCGGNCCA AAGGGAGATC CGACTCGTCT GANGGCGAAN
              841 GCGAAGANGC GGAAGANGCC GCANANCCGG CAGCAGGCCG CGGGAANGAA NGNCCGCTGG
              901 NNTGNNGGNC GAANGGNCGT ANCANAANGA NGTCCNGCGC ANANTCCAGG NGGNNACACA
              961 GGCNANCANC CNNNGNAAGG NNGTCAGCTT NCCNNANNNC ANNNCNGNAN NNGTCANNGN
            1021 CNNNNANCCG ANNNNNNNNN N
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori,Zeo,SV40 pA
            Sequence file: RDB17905_B0Aeg.seq check
            >07918_17905_B0Ae_1_SV40pro_ori_F_G11_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN CCTCTGCCTC TGANCTATTC CNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
              121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
              181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
              241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
              301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
              361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
              421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
              481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
              541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
              601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
              661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
              721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
              781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
              841 TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
              901 TATCCGCTCA CAATTCCNCA CAACATACGA NNNGNANNCN TAAAGTGNAA AGCCNGGGGT
              961 GNCTAANGAN NGAGCTAANN CACANNAATT GNNTTGCNNN NNCNGNCCGC TTTCCANNCN
            1021 GGNAANCNNN CNNGNCNANC NNCNTNNNNG NANNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Kakizawa, S., Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Sci. Rep. 10 (1): 14475 (2020). PMID 32879382.

            2023.05.02

            GNP_filter3_RDBDEP_html_230501.pl