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8xm3'Gli-BS-delta51-LucII (#RDB08062)

Reporter plasmid carring eight copies of mutated Gli-binding site

Clone info. Reporter plasmid carring eight copies of mutated Gli-binding site.
Comment Use as a negative control of 8x3'Gli-BS-delta51-LucII (cat.#08061).
Vector backbone delta51-LucII (RDB08068) (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08062 8xm3'Gli-BS-delta51-LucII DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 8xm3'Gli-BS-delta51-LucII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08062).

Reference section:

Sasaki, H., Hui, C., Nakafuku, M., Kondoh, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08062_A6Cep1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3_RV6R (Pr0228)
Region: chicken delta-crystallin Basal Pro,8xm3'Gli-BS,SV40 pA
Sequence file: RDB08062_A6Ced.seq check
>08062_08062_A6Ce_pGL3_RV6R_E09_15.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNGC CTTTCTTTAT GTTTTTGGCG TCTTCCNTTT
   61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC
  121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC
  181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGAAC AGATTCGCGA
  241 TCGAGAGCTT CTACTTCCCA CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG
  301 CTTGTCGAGA GCTTCTACTT CCCACTTCCC TGCTTGTCGA GAGCTTCTAC TTCCCACTTC
  361 CCTGCTTGTC GAGAGCTTCT ACTTCCCACT TCCCTGCTTG TCGAGAGCTT CTACTTCCCA
  421 CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG CTTGTCGAGA GCTTCTACTT
  481 CCCACTTCCC TGCTTGTCGA TCGCGAATCT GTTCCCGGGG ATCCAGACAT GATAAGATAC
  541 ATTGATGANT TTGGACAAAC CACAACTAGA ATGCANTGAA AAAAATGCTT TATTTGTGAA
  601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC
  661 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
  721 TAAAACCTCT ACAAATGTGA TATGGCTGAT CCAGACATGA TAAGANACAT TGATGAGTTT
  781 GGACAAACCA CAACTAGAAT GCANTGAAAA AAATGCNTTA TTTGTGAAAT TTGTGATGCT
  841 ATTGCTTTAT NTTGTAACCN TTANNANNCT GCAATAAACA AGTTANCAAC NANTTGCNTT
  901 TCNTTTTATG TTTCNNNNTN NNGGGGGAGG TGTGGGNANG NTTTTTTNAA NNNNNNNAAA
961 CCNNNTNNNN NGTGNNNNGN NTGNNTNCNN NNNNNNNN
//
Primer: RDB08068_F1 (Pr0221)
Region: SV40 pA
Sequence file: RDB08062_A6Cee.seq check
>08062_08062_A6Ce_RDB8068_F1_E12_05_ABI08.ab1
    1 NNNNNNNNNN NNNGNNGGNN TTCCNNGATN NTTTAAAATT ACCCTTAGAA AGCGGTCTGT
   61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG
  121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA
  181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG
  241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
  301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
  361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAANCNNN NNNANNNNNN
421 NNNN
//
Primer: Luc-C_F (Pr0331)
Region: Luc 3',small t intron,SV40 NLS
Sequence file: RDB08062_A6Cef.seq check
>08062_08062_A6Ce_Luc-C_F_D12_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCCNG NCNNGTAACN ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG
   61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG
  121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC
  181 GATGACGAAA TTCTTAGCTA TTGTAATACT CTAGAGGATC TTTGTGAAGG AACCTTACTT
  241 CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT
  301 AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT
  361 TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT
  421 GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC
  481 TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT
  541 AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC
  601 CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT
  661 TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA
  721 TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ANCTTTAGCT TTTTAATTTG
  781 TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG NCTAGAGATC NTAATCAGCC
  841 ATACNACNTT TGTAGAGGNT TTACTTGCTT TNAAAAANNT CCCACACNTC CCCCTGAACC
  901 NGAAACATNA ANNNNATGCN NNTNNNTGNT GTTAANNTNG TTNANTGCAN CTTNNAATGG
  961 NTNANAANTA AANNNNNNCA TCNCNNNTTN NNNNAANNAA NNCNNTTTTT TTCNNTGNNN
1021 TCNAGTNNTG GNTNNNNNNA NNNNNTCANN GNNTNNNATN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. [link to RRC of NBRP]
reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
user_report Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270. [link to RRC of NBRP]
user_report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014). [link to RRC of NBRP]
user_report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987. [link to RRC of NBRP]