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delta51-LucII (#RDB08068)

Reporter plasmid of chicken delta-crystallin basal promoter

Clone info. Reporter plasmid of chicken delta-crystallin basal promoter.
Comment Use empty control of 8x3'Gli-BS-delta51-LucII (cat.# RDB08061) and 8xGT-IIC-LucII (cat.# RDB08067).
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi | Kondoh, Hisato |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 13, 5206-5215 (1993).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Cell. Biol., 13, 5206-5215 (1993).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08068 delta51-LucII DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The delta51-LucII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08068).

Reference section:

Kamachi, Y., Kondoh, H., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08068_A8Ipp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Luc-B_R (Pr0790)
Region: Luc 5',chicken delta-crystallin Basal Pro,2xSV40 pA
Sequence file: RDB08068_A8Ipd.seq check
>08068_08068_B1HkS_Luc-B_R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCT CTTNNNAGCC TTATGCAGTT GCTCTCCAGC GGTTCCATCC
   61 TCTAGAGGAT AGAATGGCGC CGGGCCTTTC TTTATGTTTT TGGCGTCTTC CATTTTACCA
  121 ACAGTACCGG AATGCCAAGC TTGGCTGCCC GCACAGCCCT GCTCACCGTC TCCGCAGCTC
  181 AGCCCTGGCT GGTCGCTCCG TGCTCTCCGG TCTCACAGCC CCGCTTTTAC GTGCCCCGGA
  241 GCCTGGGCTG GCCCTGCAGG TCGACTCTAG AGGATCCAGA CATGATAAGA TACATTGATG
  301 AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG
  361 ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAATTGCA
  421 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC
  481 TCTACAAATG TGATATGGCT GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
  541 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAN GCTATTGCTT
  601 TATTTGTAAC CATTATAAGC TGCAATAAAN NAGTTNACNA CNATTGCATT NATTTTANGN
  661 TTNNNNNTCN NNGGANGTGT GGGANGNTTT TTNNAAGNNA NTAAANNNTN NNNNANNNNN
721 NNNNN
//
Primer: RDB8068_F1 (Pr0221)
Region: SV40 pA
Sequence file: RDB08068_A8Ipe.seq check
>08068_08068_A8Ip_RDB8068_F1_F12_06_ABI08.ab1
    1 NNNNNNNNNN NNNGANGGNN TTCCNNGATN NTTTAAAATT ACCCTTAGAA AGCGGTCTGT
   61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG
  121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA
  181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG
  241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
  301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
  361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT
  421 ACAAATGTGG TATGGCTGAT TATGATCTCT AGTCAAGGCA CTATACATCA AATATTCCTT
  481 ATTAACCCCT TNNNNNNTTA AAAAGCTAAA GGTACACAAT TTNTGAGCAT AGTTATTAAT
  541 AGCANACACT CTNNNNNTGT GTGGAGTAAG AAAAAACAGT ATGTTANNNN NNTAACTGTT
  601 ATGCCTACTN NNNNNGGTTA CAGAATATTT TTCCATAATT TTCTTGTNNN NNNGTGCANC
  661 TTTTTCCTNN NNNGTGTAAA TANNAAAGCA AGCAANANNN NNANNNNNNA ACACAGCATG
  721 ACTCAAANNN NNNAGCAATT CTGAAGGAAA GTCCTTGGGG TCTTNCTANC NTTNNNNNNC
781 TTTTTTGGAN GANTANAANG NTNGAGANNN NNNNNNN
//
Primer: Luc-C_F (Pr0331)
Region: Luc 3',small t intron,SV40 NLS
Sequence file: RDB08068_A8Ipf.seq check
>08068_08068_A8Ip_Luc-C_F_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA GTNNNNCCGC NNNNAGTTGC GCGGNNGNNT TGTGTTTGTG
   61 GACGAAGTAC CGAAAGGTCT TACCGGAAAA CTCGACGCAA GAAAAATCAG AGAGATCCTC
  121 ATAAAGGCCA AGAAGGGCGG AAAGTCCAAA TTGTAAAATG TAACTGTATT CAGCGATGAC
  181 GAAATTCTTA GCTATTGTAA TACTCTAGAG GATCTTTGTG AAGGAACCTT ACTTCTGTGG
  241 TGTGACATAA TTGGACAAAC TACCTACAGA GATTTAAAGC TCTAAGGTAA ATATAAAATT
  301 TTTAAGTGTA TAATGTGTTA AACTACTGAT TCTAATTGTT TGTGTATTTT AGATTCCAAC
  361 CTATGGAACT GATGAATGGG AGCAGTGGTG GAATGCCTTT AATGAGGAAA ACCTGTTTTG
  421 CTCAGAAGAA ATGCCATCTA GTGATGATGA GGCTACTGCT GACTCTCAAC ATTCTACTCC
  481 TCCAAAAAAG AAGAGAAAGG TAGAAGACCC CAAGGACTTT CCTTCAGAAT TGCTAAGTTT
  541 TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTTGCTTGC TTTGCTATTT ACACCACAAA
  601 GGAAAAAGCT GCACTGCTAT ACAAGAAAAT TATGGAAAAA TATTCTGTAA CCTTTATAAG
  661 TAGGCATAAC AGTTATAATC ATAACATACT GTTTTTTCTT ACTCCACACA GGCATAGAGT
  721 GTCTGCTATT AATAACTATG CTCAAAANTT GTGTACCTTT AGCTTTTTNA TTTGTAAAGG
  781 GGTTAATAAG GAATATTTGA TGTATAGTGC CTTGNNNAGA NNTCATAATC AGCCATACCA
  841 CATTTGTAGA NGNTTTACTT GCTTTAAAAA AACNTCCCAN ACNTCCCNNN NGNANNNTGA
  901 AACNTNAAAN NGAATGCAAT TGNNGNTGNT NNNCNTNNNT TANNGCANCN NANNNNNNNT
961 NNNNNNNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kamachi, Y., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679. [link to RRC of NBRP]