RDB08166 - pcDNA3Flag-hp38

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pcDNA3Flag-hp38

Expression vector of human p38alpha MAPK cDNA, FLAG-tagged

Catalog number	RDB08166
Resource name	pcDNA3Flag-hp38
Alternative name	pcDNAM2hp38
Clone info.	Expression vector of human p38alpha MAPK cDNA, FLAG-tagged.
Vector backbone	pcDNA3 (Plasmid)
Gene/insert name	Human p38ALPHA MAPK cDNA
Depositor\|Developer	Sudo, Tatsuhiko \|

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human p38ALPHA MAPK

External Database
Human p38ALPHA MAPK

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem. Biophys. Res. Commun., 291, 838-843 (2002).) Research purpose only.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem. Biophys. Res. Commun., 291, 838-843 (2002).) Research purpose only.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 291, 838-843 (2002).)。研究目的に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08166	pcDNA3Flag-hp38	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3Flag-hp38 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08166).

Reference section:

Sudo, T., Yagasaki, Y., Hama, H., Watanabe, N., Osada, H., Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells. Biochem. Biophys. Res. Commun., 291, 838-843 (2002). PMID 11866441.

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content	MAPK p38 inhibitor screening (English text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08166_A5Ei.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(FLAG,hp38 alpha 5') Sequence file: RDB08166_A5Eia.seq
>08166_08166_A5Ei_CMV-Forwaed_A09_03.ab1 1 NNNNNNNNNN NNNNNNCCNT NNNNNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT 121 ACGACTCACT ATAGGGAGAC CCAAGCTTGC CACCATGGAC TACAAGGACG ACGACGATAA 181 ATCGATGTCT CAGGAGAGGC CCACGTTCTA CCGGCAGGAG CTGAACAAGA CAATCTGGGA 241 GGTGCCCGAG CGTTACCAGA ACCTGTCTCC AGTGGGCTCT GGCGCCTATG GCTCTGTGTG 301 TGCTGCTTTT GACACAAAAA CGGGGTTACG TGTGGCAGTG AAGAAGCTCT CCAGACCATT 361 TCAGTCCATC ATTCATGCGA AAAGAACCTA CAGAGAACTG CGGTTACTTA AACATATGAA 421 ACATGAAAAT GTGATTGGTC TGTTGGACGT TTTTACACCT GCAAGGTCTC TGGAGGAATT 481 CAATGATGTG TATCTGGTGA CCCATCTCAT GGGGGCAGAT CTGAACAACA TTGTGAAATG 541 TCAGAAGCTT ACAGATGACC ATGTTCAGTT CCTTATCTAC CAAATTCTCC GAGGTCTAAA 601 GTATATACAT TCAGCTGACA TAATTCACAG GGACCTAAAA CCTAGTAATC TAGCTGTGAA 661 TGAAGACTGT GAGCTGAAGA TTCTGGATTT TGGACTGGCT CGGCACACAG ATGATGAAAT 721 GACAGGCTAC GTGGCCACTA GGTGGTACAG GGCTCCTGAG ATCATGCTGA ACTGGATGCA 781 TTACAACCAG ACAGTTGATA TTTGGTCAGT GGGATGCATA ATGGCCNANC TGTTGANTGG 841 AAGAACATTG TTTCCTGGTA CNGACCATAT TGATCAGTTG AAGCTCATTT TAAGANTCNT 901 TGGAACCCCN NGGGCTGANC TTTTGAAGAA AATCTNNNCA NANTCTGCAN NAAACTATAT 961 TNNGNCTTTG ANTNNNNNGN CNAANNNGAN NTTTNNNANN GNANNNNNNN NNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA,insert 3'(p38 alpha 3') Sequence file: RDB08166_A5Eib.seq
>08166_08166_A5Ei_BGH_rev_B09_06.ab1 1 NNNNNNNNNN TNNNGNNNNN NNTNGAANAG GGCCCTCTAG TGATTGTTCC CNTGAGAAGG 61 TCTTCCCCTC ACAGTGAAGT GGGATCAACA GAACAGAAAC CAGGTGCTCA GGACTCCATC 121 TCTTCTTGGT CAAGGGGTGG TGGCACAAAG CTGATGACTT CATCATAGGT CAGGCTTTTC 181 CACTCATCTA TAAGGAGGTC CCTGCTTTCA AAGGACTGAT CATAAGGATC GGCCACTGGT 241 TCATCATCAG GATCGTGGTA CTGAGCAAAG TAGGCATGTG CAAGGGCTTG GGCCGCTGTA 301 ATTCTCTTAT CTGAGTCCAA TACAAGCATC TTCTCCAGCA AGTCGACAGC CAGGGGATTG 361 GCACCAATAA ATACATTCGC AAAGTTCATC TTCGGCATCT GAGTCAAAGA CTGAATATAG 421 TTTCTTGCAG ACTCTGAGGA GATTTTCTTC AAAAGCTCAG CCCCTGGGGT TCCAACGAGT 481 CTTAAAATGA GCTTCAACTG ATCAATATGG TCTGTACCAG GAAACAATGT TCTTCCAGTC 541 AACAGCTCGG CCATTATGCA TCCCACTGAC CAAATATCAA CTGTCTGGTT GTAATGCATC 601 CAGTTCAGCA TGATCTCAGG AGCCCTGTAC CACCTAGTGG CCACGTAGCC TGTCATTTCA 661 TCATCTGTGT GCCGAGCCAG TCCAAAATCC AGAATCTTCA GCTCACAGTC TTCATTCACA 721 GCTAGATTAC TAGGTTTTAG GTCCCTGTGA ATTATGTCAG CTGAATGTAT ATACTTTAGA 781 CCTCGGAGAA TTTGGTAGAT AAGGAACTGA ACATGGTCAT CTGTAAGCTT CTGACATTTC 841 ACAATGTTGT TCANATCTGC CCCCNTGAGA TGGGTCANCA GATACACATC ATTGAATTNC 901 TNNNGNNANN NTGCNNGTGT AAAAANGNCC NACANANNNA TCNNNTTTTC NNGNTTCANA 961 TGNTTNANTA ANNNNANNNN NNTGNNNNNN NNTTNNNNAT GNNNGANNGN NNNNNANNNN 1021 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sudo, T., Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells. Biochem. Biophys. Res. Commun., 291, 838-843 (2002). PMID 11866441.

2023.05.01

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