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pRSET-hp38T106M (#RDB08174)

Bacterial expression vector of human p38alpha MAPK cDNA, inhibitor-insensitive T106M mutant, His6-tagged.

Clone info. Bacterial expression vector of human p38alpha MAPK cDNA, inhibitor-insensitive T106M mutant, His6-tagged.
Comment This mutation made BL21(DE3) host bacteria insensitive to SB202190.
Vector backbone pRSET-C (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Human p38ALPHA MAPK cDNA
Depositor|Developer Sudo, Tatsuhiko |
 
Sequence (full) RDB08174hts02.seq checkAssembled from experimentally sequenced data.
Publication Kawai, K., Anal. Biochem., 388, 128-133 (2009). [link to RRC of NBRP]
Test sheet RDB08174C0p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Anal. Biochem., 388, 128-133 (2009).) Research purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Anal. Biochem., 388, 128-133 (2009).)。 研究目的に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08174 pRSET-hp38T106M DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pRSET-hp38T106M was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08174).

Reference section:

Kawai, K., Saito, A., Sudo, T., Osada, H., A p38 mitogen-activated protein kinase inhibitor screening method using growth recovery of Escherichia coli as an index. Anal. Biochem., 388, 128-133 (2009). PMID 19248755. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kawai, K., A p38 mitogen-activated protein kinase inhibitor screening method using growth recovery of Escherichia coli as an index. Anal. Biochem., 388, 128-133 (2009). PMID 19248755. [link to RRC of NBRP]