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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET25b_Tr-cbh2-His

Expression vector of Exoglucanase 2 of Trichoderma reesei

Catalog number RDB08307
Resource name pET25b_Tr-cbh2-His
Alternative name pET25b_Tr002-His
Clone info. Biomass collection. the Exoglucanase 2 (CBH2,1,4-beta-cellobiohydrolase Exocellobiohydrolase II (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei cbh2 cDNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
Other clones in our bank

External Database
Trichoderma reesei cbh2

            Reference sequence
              
             
            Sequence RDB08307z.seq
            Remarks, protocol and/or map (pdf) RDB08307.pdf

            Distribution information

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            RDB08307 pET25b_Tr-cbh2-His DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pET25b_Tr-cbh2-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08307).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Biomass Collection (English text)
            Featured content Biomass Collection (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08307_A5Iip1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Sequence file: RDB08307_A5Iia.seq check
            >08307_08307_A5Ii_T7_20bp_G12_21.ab1
                1 TGCCGGACAT TCCCTCTAGA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGAA
               61 ATACCTGCTG CCGACCGCTG CTGCTGGTCT GCTGCTCCTC GCTGCCCAGC CGGCGATGGC
              121 CATGGATATC GGAATTAATT CGGATCCGAA TTCGAGCTCC GTCGACGTGC CTCTAGAGGA
              181 GCGGCAAGCT TGCTCAAGCG TCTGGGGCCA ATGTGGTGGC CAGAATTGGT CGGGTCCGAC
              241 TTGCTGTGCT TCCGGAAGCA CATGCGTCTA CTCCAACGAC TATTACTCCC AGTGTCTTCC
              301 CGGCGCTGCA AGCTCAAGCT CGTCCACGCG CGCCGCGTCG ACGACTTCTC GAGTATCCCC
              361 CACAACATCC CGGTCGAGCT CCGCGACGCC TCCACCTGGT TCTACTACTA CCAGAGTACC
              421 TCCAGTCGGA TCGGGAACCG CTACGTATTC AGGCAACCCT TTTGTTGGGG TCACTCCTTG
              481 GGCCAATGCA TATTACGCCT CTGAAGTTAG CAGCCTCGCT ATTCCTAGCT TGACTGGAGC
              541 CATGGCCACT GCTGCAGCAG CTGTCGCAAA GGTTCCCTCT TTTATGTGGC TAGATACTCT
              601 TGACAAGACC CCTCTCATGG AGCAAACCTT GGCCGACATC CGCACCGCCA ACAAGAATGG
              661 CGGTAACTAT GCCGGACAGT TTGTGGTGTA TGACTTGCCG GATCGCGATT GCGCTGCCCT
              721 TGCCTCGAAT GGCGAATACT CTATTGCCGA TGGTGGCGTC GCCAAATATA AGAACTATAT
              781 CGACACCATT CGTCAAATTG TCGTGGAATA TTCCGATATC CGGACCCTCC TGGTTTATTG
              841 AGCCTGACTC TCTTGCCAAC CTGGTGACCA ACCTCGGTAC TCCAAAGTGT GCCAATGCTC
              901 AGTCAGCCTA CCTTGAGTGC ATCAACTACG CCCGTCACAC AGCTGAACCT TCCAAATGTT
              961 GCGATGTATT TGGACGCTGG CCATGCAGGA TGGCTTGGCT GGCCGGCAAA CCAGACCCCG
             1021 CCGCTCAGCT ATTTGCAATG TTACAGATGC ATCGTCTCGA GAGCTCTTCG CGATGCCACA
            1081 TGTCGCACTA CACGGTGACA TACCAGCCTC CATCGTACAC GCCAGCCAAC GCTGATC
            //
            Primer: f1ori_R3
            Sequence file: RDB08307_A5Iib.seq check
            >08307_08307_A5Ii_f1ori_R3_H12_24.ab1
                1 GAATCGCATC GGATTAGTTC CTCCTTTCAG CAAAAACCCC TCAAAGACCC GTTTAGAGGC
               61 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG
              121 GGCTTTGTTA GCAGCCGGAT CTCAGTGGTG GTGGTGGTGG TGCTCGACAT CCTCGGGGTC
              181 TTCCGGGGCG AGTTCTGGCT GGCTAGCCCG TTTGATCTCG AGTGCGGCCG CAAGCTTGTC
              241 GACCAGGAAC GATGGGTTTG CGTTTGTGAG AAGCTGCACA AAGTAGGCTT GGAACCAAGC
              301 ACCAGCTTGA GGCGCCGGTT GCAAGGCATC TGGGAGCGCA CAGTGGGAGT CAAATCGTGG
              361 CGCACTGCTG TCGCTGGTGC CGTCACACTC GCCGCCTGGC TTGACCCAGA CAAACGAATC
              421 CAGCAACGAG TCCCCAGTGT TTGCGGATGG GCGAATACCA AATCCGGTGC CGATCACATT
              481 GCACCAGTCT CCCCACTGTT GCTGTCCGGT AGGCTGCTTT CCCGATCGAC CTTGATCAGT
              541 GATGAAGAAG GCGTTGGACC AGCCGTGATT GGCAAGAAGA GGTCCAATAG CGTGGATGTA
              601 CAGCTTCTCG TTGTAGACAG CGTTGCCTTG CGTGTACGAT GGGGGGCTGG TAATGTTCCA
              661 CCCGTTGTAG TTGGCGACAT TGGTTGCCAA TCCGCGAAGA GCTCTCGGAG ACGATGCATT
              721 CTTGTAAACA TTTGCAAATA GCTGAGCGGC CGGGTCTTGG TTTGCCGGCC AGCCAAGCCA
              781 TCCTGCATGG CCAGCGTCCA AATACATCGC AACATTTGGA AGGTTCAGCT GTGTGACGGC
              841 GTAGTTGATG CACTCAAGGT AGGCTGACTG AGCATTGGCA CACTTTGGAG TACCGAGGTT
              901 GGTCACCAGG TTGGCAAGAG AGTCAGCTCA ATAACCAGGA GGGTCCGGAT ATCGGAATAT
              961 TCCCACGACA ATTTGACGAA TGGTGTCGAT ATAGTTCCTT ATATTTGGCG ACGCCACCAT
             1021 CGGCATAGAG TATTCGCCAA TTCGAAGCAG GGCAGCGCAT CGGCGATCCC GGCAAGTCAA
            1081 TACATCCAAC CAAACTGTCA GGCATAAGTT ACGGCAATCT ATGTAGAC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl