Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G4-1a (#RDB08396)

Bacteria expression vector of human galectin-4, GST fusion protein

Clone info. Bacterial expression clone of human galectin-4.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-4 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08396z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースの利用は、学術機関の学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08396 pGEX-G4-1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G4-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08396).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08396_A9Hsp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: GST-M_F (Pr0153)
Region: GST 3', thrombin site, insert5'
Sequence file: RDB08396_A9Hsa.seq check
>08396_08396_A9Hs_1_GST-M_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNNNNNTTG NNTTTCTTAG CAAGCTACCT GAAATGCTGA
   61 AAATGTTCGA AGATCGTTTA TGTCATAAAA CATATTTAAA TGGTGATCAT GTAACCCATC
  121 CTGACTTCAT GTTGTATGAC GCTCTTGATG TTGTTTTATA CATGGACCCA ATGTGCCTGG
  181 ATGCGTTCCC AAAATTAGTT TGTTTTAAAA AACGTATTGA AGCTATCCCA CAAATTGATA
  241 AGTACTTGAA ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG
  301 GTGGTGGCGA CCATCCTCCA AAATCGGATC TGGTTCCGCG TGGATCCCCA GGAATTCCCA
  361 TGGCCTATGT CCCCGCACCG GGCTACCAGC CCACCTACAA CCCGACGCTG CCTTACTACC
  421 AGCCCATCCC GGGCGGGCTC AACGTGGGAA TGTCTGTTTA CATCCAAGGA GTGGCCAGCG
  481 AGCACATGAA GCGGTTCTTC GTGAACTTTG TGGTTGGGCA GGATCCGGGC TCAGACGTCG
  541 CCTTCCACTT CAATCCGCGG TTTGACGGCT GGGACAAGGT GGTCTTCAAC ACGTTGCAGG
  601 GCGGGAAGTG GGGCAGCGAG GAGAGGAAGA GGAGCATGCC CTTCAAAAAG GGTGCCGCCT
  661 TTGAGCTGGT CTTCATAGTC CTGGCTGAGC ACTACAAGGT GGTGGTAAAT GGAAATCCCT
  721 TCTATGAGTA CGGGCACCGG CTTCCCCTAC AGATGGTCAC CCACCTGCAA GTGGATGGGG
  781 ATCTGCAACT TCAATCAATC AACTTCATCG GANGCCAGCC CCTCCGGCCC CAGGNNCCCC
  841 CGATGATGCC ACCTTACCCT NGTCCCGGAC ATTGCCATCA ACAGCTGAAC AGCCTGCCNA
901 CCATGGANNG
//
Primer: pJB8-Forward (Pr0084)
Region: insert3'
Sequence file: RDB08396_A9Hsb.seq check
>08396_08396_A9Hs_1_JB8-Forward_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG GGTNNNGGCT GCGCCCCGAC ACCCGCCNAC ACCCGCTGAC
   61 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC
  121 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGATCGTC
  181 AGTCAGTCAC GATGCGGCCG CTCGAGTTAG ATCTGGACAT AGGACAAGGT GACATCACCC
  241 TGGATTTCCA ATGTGTCCAC CCTCTGGAAG GCCGAGAGGC GATGGGCAAA GTCAAAGAGG
  301 TGCTGGCCAT TGGCGTAAAC CTTGAAGCGA TCCAAGCCAC AGCGAATGGA CAGATCAAAG
  361 AACTGTCCGG GACCAAATGG GTTGTGGGTG ATCTTCTTCT CCTCGGATCC CCACGAGCCA
  421 TTCAGAAGGC TGTTCCGGAC CACGGTACCG TTGCCCATGC GGGGATTAAT GTGCAGAGCT
  481 ATGTCCCCTG AGGAGCCCAC CTTGAAGTTG ATAGCAAAGC TCTTGCCTGT GGGAGGCACA
  541 TAGCCCTTGA TGATGATGGT TCTTCGAGCT GTGAGCCCTC CTTGCAGCCT CCCGAAATAT
601 GGCACAGGCG GGTTGAAGGT TGGGGGTCCT TCCATGGTGG GCANG
//
Primer: pUC-M13_-100 (Pr0310)
Region: lacZ alpha, lacO, lacI gene
Sequence file: RDB08396_A9Hsc.seq check
>08396_08396_A9Hs_1_pUC-M13_-100_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNGCNNG GNNTTTCCNN GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGA ATCCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
  121 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
  181 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
  241 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
  301 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
  361 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
  421 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
  481 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
  541 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
  601 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
  661 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
  721 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC
  781 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGANGG GTGTCTGGTC
841 ANAGANATCA AGAAATAACG CCGGANNNNT ANTGCAGGCA GCTTCCACAG CAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]