RDB08399 - pET-G7-1a

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pET-G7-1a (#RDB08399)

Bacteria expression vector of human galectin-7, tag-free protein

Clone info.	Bacterial expression clone of human galectin-7.
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone	pET-11a (Plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	Human galectin-7 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Sequence	RDB08399z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、学術機関の学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08399	pET-G7-1a	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-G7-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08399).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08399_A9Hsp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-R1 (Pr0723) Region: T7 ter, insert, lacO, T7pro Sequence file: RDB08399_A9Hsa.seq
>08399_08399_A9Hs_2_pET-check_R1_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN AGNCNGGCNC CGTGTATGAA ATCTAACAAT 61 GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG 121 CCGGTACTGC CGGGCCTCTT GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC 181 CCCTCAAGAC CCGTTTAGAG GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG 241 CAGCCAACTC AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCCTCAGAA GATCCTCACG 301 GAGTCCAGCT GCACGTCCCC GCCCACCTCC ACCAGGCGCA CGCGCGCCAG CGGCAGGCGG 361 TGGCGGAAGT GGTGGTACTG GGCGTCCCCA ACCACGGCCT TGAAGCCGTC GTCTGACGCG 421 ATGATGAGCA CCTCGAAGGG CTGCCCGCGC TGGAAAGGAA CGCCCGGCCC GCGCTCCTCG 481 CGGCCCCAGG AGCCTTGCTC CTTGCTGTTG AAGACCACCT CCGACGTGTC CAGCCGGGGG 541 TTGAAATGCA GGGCGGCATC GGAGCCCTGC TCCTCCCCGC ACAGCAGGTT TACATGGAAC 601 CTGCTGGCAT TGGGAGGAAC CAAGCCGCGA ATTCTCAGCA CCGTGCCAGG GCGGATGCCC 661 TCGGGCAGCG AGGACTTGTG GGGGACGTTG GACATATGTA TATCTCCTTC TTAAAGTTAA 721 ACAAAATTAT TTCTAGAGGG GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCNTA 781 TTNATTTCGC GGGATCGAGA TCTCGATCCT CTACNNCCNG ANGCATCGTG GCCGGCATCA 841 CCGGCGCNNC ANGTGCGGNT GCTGGCGCNN ATATCGCCNA NATCNNN //
Primer: lacIpro_F2 (Pr0647) Region: lacIpro, lacI Sequence file: RDB08399_A9Hsb.seq
>08399_08399_A9Hs_2_lacIpro_F2_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNA NGNNTCGCAT AAGGGAGAGC GTCGAGNTCC CGGACACCAT 61 CGAATGGCGC AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG 121 GGTGGTGAAT GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA 181 TCAGACCGTT TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA 241 AGTGGAAGCG GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 301 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC ACGCGCCGTC 361 GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC 421 GATGGTAGAA CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA 481 ACGCGTCAGT GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA 541 AGCTGCCTGC ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 601 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT 661 GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGTCTGCG 721 TCTGGCTGGC TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG CGGAACGGGA 781 AGGCGACTGG AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT 841 CGTTCCCACT GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCNA TGCGCGCCAT 901 TACCGANTNC GGGCTGCNCG TTGGTGCGGA TATCTCNN //