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pGEX-G8(M) R233H-2a (#RDB08402)

Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with an R233H mutation, GST fusion protein


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Bacterial expression clone of human galectin-8 with an R233H mutation.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Growth conditions 37C, LB-broth+Amp
Gene/insert name Human galectin-8 cDNA
Depositor|Developer Nishi, Nozomu |

Distribution information

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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
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Catalog # Resource name Shipping form Fee
RDB08402 pGEX-G8(M) R233H-2a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGEX-G8(M) R233H-2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08402).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [Article] [RRC of NBRP]