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pET-G9(S)-3a (#RDB08416)

Bacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), tag-free protein

Clone info. Bacterial expression clone of human galectin-9 (short linker type).
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pET-11a (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Human galectin-9 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08416z.seq

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08416 pET-G9(S)-3a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-G9(S)-3a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08416).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08416_A4Ek.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB08416_A4Eka.seq check
>08416_08416_A4Ek_T7_20bp_G04_19.ab1
    1 GCGGAACAGT TCCCTCTAGA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGGC
   61 CTTCAGCGGT TCCCAGGCTC CCTACCTGAG TCCAGCTGTC CCCTTTTCTG GGACTATTCA
  121 AGGAGGTCTC CAGGACGGAC TTCAGATCAC TGTCAATGGG ACCGTTCTCA GCTCCAGTGG
  181 AACCAGGTTT GCTGTGAACT TTCAGACTGG CTTCAGTGGA AATGACATTG CCTTCCACTT
  241 CAACCCTCGG TTTGAAGATG GAGGGTACGT GGTGTGCAAC ACGAGGCAGA ACGGAAGCTG
  301 GGGGCCCGAG GAGAGGAAGA CACACATGCC TTTCCAGAAG GGGATGCCCT TTGACCTCTG
  361 CTTCCTGGTG CAGAGCTCAG ATTTCAAGGT GATGGTGAAC GGGATCCTCT TCGTGCAGTA
  421 CTTCCACCGC GTGCCCTTCC ACCGTGTGGA CACCATCTCC GTCAATGGCT CTGTGCAGCT
  481 GTCCTACATC AGCTTCCAGA CCCAGACAGT CATCCACACA GTGCAGAGCG CCCCTGGACA
  541 GATGTTCTCT ACTCCCGCCA TCCCACCTAT GATGTACCCC CACCCCGCCT ATCCGATGCC
  601 TTTCATCACC ACCATTCTGG GAGGGCTGTA CCCATCCAAG TCCATCCTCC TGTCAGGCAC
  661 TGTCCTGCCC AGTGCTCAGA GGTTCCACAT CAACCTGTGC TCTGGGAACC ACATCGCCTT
  721 CCACCTGAAC CCCCGTTTTG ATGAGAATGC TGTGGTCCGC AACACCCAGA TCGACAACTC
  781 CTGGGGGTCT GAGGAGCGAA GTCTGCCCCG AAAAATGCCC TTCGTCCGTG GCCAGAGCTT
  841 CTCAGTGTGG ATCTTGTGTG AAGCTCACTG CCTCAAGGTG GCCGTGGATG GTCAGCACCT
  901 GTTTGAATAC TACCATCGCC TGAGGAACCT GCCCACCATC AACAGACTGG AAGTGGGGGG
  961 CGACATCCAG CTGACCCATG TGCAGACATA GAGATCCCGG CTGCTAACAA AGCCCGAAAG
 1021 GAAGCTGAGT TGGCTGCTGC CACGCTGAGC ATACTAGCAT AACCCCTTGG GGCCTCTAAA
 1081 CGGGTCTGAG GGTTTTTGCT GGAATGAGAC TATATCAGAT ATCCCGCAGA GGCGGCAGTA
1141 CGGCATACCA GCCTATGCCT TACAGCCATC AGGTTGAACG GGGTGGTCCG AGGA
//
Primer: T7_terminator
Sequence file: RDB08416_A4Ekb.seq check
>08416_08416_A4Ek_T7terminator_H04_22.ab1
    1 AATTTCGCCT TCCTTTTCGG GCTTTGTTAG CAGCCGGATC TCTATGTCTG CACATGGGTC
   61 AGCTGGATGT CGCCCCCCAC TTCCAGTCTG TTGATGGTGG GCAGGTTCCT CAGGCGATGG
  121 TAGTATTCAA ACAGGTGCTG ACCATCCACG GCCACCTTGA GGCAGTGAGC TTCACACAAG
  181 ATCCACACTG AGAAGCTCTG GCCACGGACG AAGGGCATTT TTCGGGGCAG ACTTCGCTCC
  241 TCAGACCCCC AGGAGTTGTC GATCTGGGTG TTGCGGACCA CAGCATTCTC ATCAAAACGG
  301 GGGTTCAGGT GGAAGGCGAT GTGGTTCCCA GAGCACAGGT TGATGTGGAA CCTCTGAGCA
  361 CTGGGCAGGA CAGTGCCTGA CAGGAGGATG GACTTGGATG GGTACAGCCC TCCCAGAATG
  421 GTGGTGATGA AAGGCATCGG ATAGGCGGGG TGGGGGTACA TCATAGGTGG GATGGCGGGA
  481 GTAGAGAACA TCTGTCCAGG GGCGCTCTGC ACTGTGTGGA TGACTGTCTG GGTCTGGAAG
  541 CTGATGTAGG ACAGCTGCAC AGAGCCATTG ACGGAGATGG TGTCCACACG GTGGAAGGGC
  601 ACGCGGTGGA AGTACTGCAC GAAGAGGATC CCGTTCACCA TCACCTTGAA ATCTGAGCTC
  661 TGCACCAGGA AGCAGAGGTC AAAGGGCATC CCCTTCTGGA AAGGCATGTG TGTCTTCCTC
  721 TCCTCGGGCC CCCAGCTTCC GTTCTGCCTC GTGTTGCACA CCACGTACCC TCCATCTTCA
  781 AACCGAGGGT TGAAGTGGAA GGCAATGTCA TTTCCACTGA AGCCAGTCTG AAAGTTCACA
  841 GCAAACCTGG TTCCACTGGA GCTGAGAACG GTCCCATTGA CAGTGATCTG AAGTCCGTCC
  901 TGAGACCTCC TTGAATAGTC CCAGAAAAGG GGACAGCTGG ACTCAGTAGG GAGCCTGGGA
  961 ACCGCTGAAG GCCATATGTA TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG
 1021 GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA
 1081 TCTCGATCCT CTACGCCGGG ACGCATCGTG GCCGGCATCA CCGGGCGCCC ACAGGTGCGA
 1141 TGGCTGCGGC TATTATCGTC GAACATCCAC GAATTGGGAA GGATCCGGGC TCCGCCACTT
1201 CCCGGCCTTC AATTGAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]