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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

316-mRFP-SED5 (#RDB08659)

Plasmid clone to express mRFP-SED5 in S. cerevisiae

Alternative name 115
Clone info. Plasmid clone to express mRFP-SED5 (TDH3 promoter) in S. cerevisiae.
Comment mRFP-SED5 (TDH3 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SED5
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08659z.seq
Remarks, protocol and/or map (pdf) RDB08659.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature 441,1007-1010, 2006)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08659 316-mRFP-SED5 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 316-mRFP-SED5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08659).

Reference section:

Matsuura-Tokita, K., Takeuchi, M., Ichihara, A., Mikuriya, K., Nakano, A., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08659_A4Iop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: insert 5'(TDH pro)
Sequence file: RDB08659_A4Ioc.seq check
>08659_08659_B1KiF_M13_-40_D02_11_ABI24.ab1
    1 NNNNNNNNNG NNGNANTGGA GCTCCNCCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC
   61 GGAGATTCCT TGATTACGTA AGGGAGTTAG AATCATTTTG AATAAAAAAC ACGCTTTTTC
  121 AGTTCGAGTT TATCATTATC AATACTGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA
  181 GTGATTTTCC TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA
  241 CATGCCCAAA ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA
  301 CAGTTTATTC CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA
  361 AAAAAAAAGA ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC
  421 CATTCTCTTA GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC
  481 TCAATGGAGT GATGCAACCT GCCTGGAGTA AANNNNNNCA CAAGGCAATT GACCCACGCA
  541 TGTATCTNTC TCANTNNCNT ACACCTTCTA NTANCTTCTG CTCNCTCTGA TNNGNAAAAA
601 NCTGANNANN NNNGNTGAAA CCANTTCCCT GANATNNNTC CCNNACNNGN NNNNNNNNN
//
Primer: Reverse-2 (Pr0100)
Region: insert 3'(CMK1 ter 3')
Sequence file: RDB08659_A4Iod.seq check
>08659_08659_B1KiF_reverse2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNCNNNAAGG GAACAAAAGC TGGTACCGGG CCCCCCCTCG AGGTCGACGG
   61 TATCGATGAG TGCCCAATTC AAATTGTTTT ACCATGACAC ACCATCGTTG GCGCATGATG
  121 TTGCACGAGG CAGAAATACA TCGGGGAAGG TTTATTGGTA TGAATTATGC CACGATTCAG
  181 CAACACTGCT GGAGTTCTTG GAGTTCTTGG ACTATAAAAT TGTTAAACCT CAAGATGAGA
  241 AAAAGGAGGG AAATGAAAAA GAAAAAGAAG CACTGAATAA TGAAGCGCAC ATATTGGAGC
  301 AGAAATCTAC CACTGATAAT AATCCCTCCA TAAATACAAA CCCGCTACCA AAAGACGCAA
  361 AATATAACAC TGCGAGAAAG AAGTTACAGA TATTAAAGGA ATTTTTATCT GATTACTATT
  421 TCATTCTTCG TCAGTTTGAA CAAATGAAAG TGCAATTTGC AGACATGAAG CCAGGCAAGA
  481 GACAGTTGAG AAGGATACAG CGACAGACTG TAAACTATAA TACGGAATAT GATAGCGAGN
  541 ANNNNGTCGA TGANGNAANA NNACGACNAA NNTGANNNTT TATGANGACN ACGANNATGN
  601 NNCTTCNTTT GANGNNNGNT AGANTTAAAA AGGNNNNGCA NTNNNACNAN NNNNNNNTNN
661 NNNNNNNN
//
Primer: Red-C_F (Pr0432)
Region: insert mid(mRFP1 3',sed5 5')
Sequence file: RDB08659_A4Ioe.seq check
>08659_08659_B1KiF_Red-C_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNAGCGCGCC GAGGGCCGCC ACTCCACCGG CGCCAGATCT
   61 ATGAACATAA AGGATAGAAC TTCAGAATTT CAACAAAGTG TGTTGAGTTA CAAGAAAAGA
  121 AACAAAAACT TTAGAGAACA GCAGAGGGAA CGTCTACAAN AGAAAGAAAG TGAAAACTTT
  181 GCCAACAACA CAACCGGCAA TGGGAAAAGT GTCTCTGAGT TTCAAAAAAA GGCTTCTGGC
  241 ATAGCTCACN AAATTTCATC CACCGCACAA TTATTATCNA AATTANCANT GNTGGNCANN
  301 NNGAANNCGA TGTTCANTGA NNACCCTGNA NANANNGNCN ANNNNTCNNT NTTGATNNNN
361 NNGAANANNN NNGCCNNNNN NNNNN
//
Primer: CMK1ter_R (Pr0015)
Region: insert mid(CMK1 ter 5',sed5 3')
Sequence file: RDB08659_A4Iof.seq check
>08659_08659_A4Io_CMK1ter_R_F04_16.ab1
    1 NNNNNNNNNT NTNNNNTTNC GNNAGCACTG TGTTAACTAA TTGTGCAAAA GCTTAGATCT
   61 TTAATTGACT AAAACCCAAA TAACGAAAAA TACAAAGATT ATAAAAAAAA CCTTTGCGGC
  121 TAACCATCTA TTACTCTTTA TCCTGTCGAA GTATTTCAAA AGTTCCCTTT GAGCACCACT
  181 AATGTTTAAA TCGATGTCGT CTACGTTTGC ATCAATCCTT TGGATTACTT CGCCCTGTTC
  241 TTGAACCATG GAGGCCAGTT GTTGGAAGAG ATTTCCCACT TCCTGGATTG TGGATTCTAT
  301 TGTCTCCACC GCCCTATTTC TTTCTTGTAA GTAGACGTTA TTGGATAACT GTCCCTCTTC
  361 CATTAACATT AGTTGAGAGT CGTTTTGAGG GAAAGACAGT TCTCCTTGGT TGGACGAGTT
  421 GTTATTCTTT TCTGAGGATT CATCTAGTAG TGAGGTCATG AATGGGTTGG AGTTGTTATA
  481 CGTGGTCAAG TCCGCGGCGT GATTGCTTTG CGTTTGGTCA TCAGCTGGTG CATGTCCAGT
  541 GTCGGTGGTT AGTTTTTGCC ATCTGTCTTT GTTAGCCATT TCTAGTCGTT GCCTTTCCTC
  601 CAATACGTCT TTAAAACTTC CTGATATGTT TTTCATTTGC GTGTTTAAAA GATTTACGAC
  661 ATTTTTGGAG TGCTGTACGG CACTAGGTTG CTTAGAAGAT TGATTTGATG TGTTGCCATT
  721 CACGTCGGTT TTCTTGAGCT GACTTAATTG CACTAAACTT TGTTCGATGG CATAAATCTT
  781 CCTTTTGATC AAAAATGATA GCTCGGCNAT CTCTACAGGG TTGTCATTGA ACATCNGTTT
  841 CCTTTNGGCC NGCACTGCTA ATTTCNATNA TAATTGTGCG GNGGANGAAA TTTCGNGANC
  901 TATGCNNGAA NCNNTTTTTT TGAAACTNNG ANACNCTTTT CCNNTTGNNN GNTNNNGNNN
961 NNGGCNAANT TTTCNCTTTN NTTCTNNNNN ANANNNNNCN NCNNNNNNNN NN
//
Primer: TDH3pro_F (Pr0431)
Region: insert mid(TDH pro 3',mRFP 5')
Sequence file: RDB08659_A4Iog.seq check
>08659_08659_A4Io_TDH3pro_F_E04_13.ab1
    1 NNNNNNNNNN NNGNNNTGNN TGNANTTCTN TAAATCTATT TCTTAAACTT CTTAAATTCT
   61 ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAA GAACTTAGTT TCGAATAAAC
  121 ACACATAAAC AGAATTCATG GCCTCCTCCG AGGACGTCAT CAAGGAGTTC ATGCGCTTCA
  181 AGGTGCGCAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG
  241 GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGC GGCCCCCTGC
  301 CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCCAGTACGG CTCCAAGGCC TACGTGAAGC
  361 ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC
  421 GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG
  481 ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG
  541 TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCACCGA GCGGATGTAC CCCGAGGACG
  601 GCGCCCTGAA GGGCGAGATC AAGATGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG
  661 CCGAGGTCAA GACCACCTAC ATGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAAGA
  721 CCGACATCAA GCTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG
  781 AGCGCGCCGA GGGCCGCCAC TCCNCCGGCG CCNGATCTAT GAACATAAAG GATAGAACTT
  841 CNNAATTTCN ACNAAGTGTG TTGAGTTACN AGAAAAGAAA CAAAAACTTT ANAGAANNGC
  901 ANANGGAACG TCTACNANAN AAAGNNNGTG AAAACNTTGC CNACNACNCN ACNNGNNNNG
  961 GGAAAANNGN CTCNNNNNTN NNNAAAANNN NNNNNGGNNN ANCNNNNNAN NTTNNNNNNN
1021 NNN
//
Primer: phRLR1 (Pr0219)
Region: AmpR pro,CEN_ARS
Sequence file: RDB08659_A4Ioh.seq check
>08659_08659_B1KiF_phRLR1_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGGGTCCTTT
   61 TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA TATAAATTAA
  121 AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG AANATGTAAA
  181 AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT GCTCTCAGGT
  241 ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA ATCCTGTGAT
  301 TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT TCTTGTCTAA
  361 NAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AANNNTTTGT TTATTTTTTT
  421 TTCTTCATTC CGNAANTCTT CNNNNNNNTT TATTTACTTT NNANNNNNNN NNTNNNNNNC
481 NNNNAANNNN NNNNNN
//
Primer: URA3pro_F (Pr0170)
Region: URA3 pro,URA3 5'
Sequence file: RDB08659_A4Ioi.seq check
>08659_08659_B1KiF_URA3pro_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA
   61 TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA CTCATCCTAG TCCTGTTGCT
  121 GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT TGTGTGCTTC ATTGGATGTT
  181 CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG GTCCCAAAAT TTGTTTACTA
  241 AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG GCACAGTTAA GCCGCTAAAG
  301 GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA GAAAATTTGC TGACATTGGT
  361 AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA TAGCAGAATG GGCAGACATT
  421 ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA
  481 GAAGTAACAA AGGAACCTAN AGGCCTTTTG ATGTTAGCAG AATTGTCATG CAAGGGCTCC
  541 CTATCTACTG GANAATATAC TAAGGGTACT GTTGACNTTG CNAANNANCN ACNAANNNTT
  601 TGNTANCGNN TTNANTNGCT NNAANAGNNA TGGGNGGNAA GANANGNNNG NNNNNAATTG
  661 GTNNNNNNNN ACNCCCNNNN NNGNNNTTAN ANGNNNNNGN ANANNCNTNN NNNNCNANNN
721 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]