RDB08661 - 316-mRFP-GOS1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

316-mRFP-GOS1 (#RDB08661)

Plasmid clone to express mRFP-GOS1 in S. cerevisiae

Alternative name	117
Clone info.	Plasmid clone to express mRFP-GOS1 (TDH3 promoter) in S. cerevisiae.
Comment	mRFP-GOS1 (TDH3 promoter) on pRS316 (pCEN, URA3)
Vector backbone	pRS316 (Plasmid)
Selectable markers	Amp
Gene/insert name	S. cerevisiae GOS1
Depositor\|Developer	Nakano, Akihiko \|

Sequence	RDB08661z.seq
Remarks, protocol and/or map (pdf)	RDB08661.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature 441,1007-1010, 2006)。
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08661	316-mRFP-GOS1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 316-mRFP-GOS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08661).

Reference section:

Matsuura-Tokita, K., Takeuchi, M., Ichihara, A., Mikuriya, K., Nakano, A., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08661_A4Io.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TDH3pro_F Sequence file: RDB08661_A4Ioa.seq
>08661_08661_A4Io_TDH3pro_F_G04_19.ab1 1 ATAGGACTGG TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC 61 TACTTTTATA GTTAGTCTTT TTTTTAGTTT TAAAACACCA AGAACTTAGT TTCGAATAAA 121 CACACATAAA CAGAATTCAT GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC 181 AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG 241 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG 301 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCCAGTACG GCTCCAAGGC CTACGTGAAG 361 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG 421 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG 481 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC 541 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG AGCGGATGTA CCCCGAGGAC 601 GGCGCCCTGA AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC 661 GCCGAGGTCA AGACCACCTA CATGGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAG 721 ACCGACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC 781 GAGCGCGCCG AGGGCCGCAC TCCACCGGCG CCAGATCATG AGCTCACAAC GTCTTTCGTC 841 ACATAAGGGG CAAGGCCATT TCTCTAGAAA ACACAAACGG AGTCACTTCT TTCCAGTACT 901 CACATTCGCG CAGAACACTA GTCAGAACCA ACTGCAAGAG AGATGACAGC AGTGGAAGGA 961 TTCTAGCTGA GAATGAATGT AATCGATTCA TAACAAATTC TGGAATCTAG CTGGAATTCC 1021 GGCCTAGCTG CCATGACGTC AGACTCAGAC TGGAAGCTCC GAGTCGGTC //
Primer: CMK1ter_R Sequence file: RDB08661_A4Iob.seq
>08661_08661_A4Io_CMK1ter_R_H04_22.ab1 1 GATTCTAATT TTTCCTTTTC GGCAGCACTG TGTTAACTAA TTGTGCAAAA GCTTAGATCC 61 TTACCATGTG AAAAACAAAA ACAGTATACA AAGGGTGGTT ATCGTGGCCA ATACAAACGC 121 GTTTTTCTTC CTTCTCGTGT TAATCTTCAT AATAAGCTGA TTGACACCTG GTATTCTTTG 181 TAAAGTTTGC AACACCTTGT TGTTTGCCGT GTTCAGCACG TTGCTCTGCG AGTGGAACTG 241 CGACCGAGTC TCCCAGGCTT GGGAGATGAG ACGGTCGACT ACGTTGTTGG ACTGATCGAT 301 TCGCCTTGTT TCGTTCTGAA TGTACTCGTC AGCATCCCCT ATAGGAGCTG GCGCGTCCGT 361 TGTGGAATTA GCAATATCGT TCTTCACGCT GAATAACAAG TTTAGTCTGT TGCGCTCTTG 421 CTGGATGGAG GACCTGATAT TGCGGAAGCT CTTCCAGTGG TCTTGTAAGA TCTCCTTGTG 481 GCGGTGCAGT TGGGAAAGCT TTGAGGCGGA GATTGCCGGG TTAGAATCGC AGATTTGTGT 541 TAATGAATCG ATTACATCCT GTCTCTGGCC TAAGATTCCC TCCAACTGCT TGTCTATCTT 601 CTTTTCTTGG CCAGTTTGTT CTGAACTAGT TGTCTGCGCG AATGTGGAGT ACTTGGAAAG 661 AAGTGACTCC GTTTGTGTTT CTAGAGAAAT GGCCTTGCCC CTTATGGTGA CGAAAGACGG 721 TTGTGAGCTC ATGGATCTGG CGCCGGTGGA GTGGCGGCCC TCGGCGCGCT CGTACTGTTC 781 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGATGTCGG TCTTGTAGGC 841 GCCGGGCAGC TGCACGGGCT TCTTGGCCAT GTAGGTGGTC TTGACCTCGG CGTCGTAGTG 901 GCCGCCGTCC TTCAGCTTCA GCCTCATCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG 961 GTACATCCGC TCGGTGGGAG GCCTCCCAGC CCATGGGTCT TCTTCTGCAT TACGGGGCGT 1021 CGGAGGGGAA GTTGGTGCCG CGCAGCCTTC ACCTTGTAGA TGACTCGCCG TTCTTGCATG 1081 AAGAAGTCTG GGTCACGGGT TCACACGTGC CGGTTCTTCG AAGGTCATCA GTCGCATTCC 1141 ACATGAGCTC AGGAGACAGG CATTCCAAG //