Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS lacZ (#RDB09309)

Expression clone of lacZ.

Alternative name stock no 240
Clone info. Expression clone of lacZ under the control of CAG promoter.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name E. coli lacZ genomic DNA
Depositor|Developer Niwa, Hitoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09309 pCAGGS lacZ DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS lacZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09309).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09309_A9Fcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 ()
Sequence file: RDB09309_A9Fca.seq check
>09309_09309_A9Fc_2_CAG_R1_C05_08_ABI24.ab1
    1 TGAAACGCGG CCGCACCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA
   61 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA
  121 GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT
  181 CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT
  241 CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA
  301 AGAATTCAAG CTTGGGATCT CTATAATCTC GCGCAACCTA TTTTCCCCTC GAACACTTTT
  361 TAAGCCGTAG ATAAACAGGC TGGGACACTT CACATGAGCG AAAAATACAT CGTCACCTGG
  421 GACATGTTGC AGATCCATGC ACGTAAACTC GCAAGCCGAC TGATGCCTTC TGAACAATGG
  481 AAAGGCATTA TTGCCGTAAG CCGTGGCGGT CTGGTACCGG TGGGTGAAGA CCAGAAACAG
  541 CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG CGAGATCGAT
  601 CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT
  661 GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT
  721 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA
  781 GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC
  841 TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT
  901 ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT
  961 AATGTTGATG AAAGCTGGCT ACAGAAGGCC AGACGCGAAT TATTTTTTGA TGGCGTTAAC
 1021 TCGGCGTTTC ATCTGTGGTG CAACGGGCGC CTGGGTCGGT TACGGCCAGG ACAGGTCGTT
 1081 TTGTCGTCCT GGAATTGGAA CATGAAGCGC CAGTTTTTAC GCCCGCCCCC GGGAAGAAAA
1141 AAAA
//
Primer: pCAGGS_R1 ()
Sequence file: RDB09309_A9Fcb.seq check
>09309_09309_A9Fc_2pCAGGS_R1_D05_11_ABI24.ab1
    1 AAAAATTCCA ACACACTATT GCAATGAAAA TAAATTTCCT TTATTAGCCA GAAGTCAGAT
   61 GCTCAAGGGG CTTCATGATG TCCCCATAAT TTTTGGCAGA GGGAAAAAGA TCTCAGTGGT
  121 ATTTGTGAGC CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT
  181 GAGGAGTGAA TTCCTCGACG GATCCCCCCT GCCCGGTTAT TATTATTTTT GACACCAGAC
  241 CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATTCC GCCGATACTG ACGGGCTCCA
  301 GGAGTCGTCG CCACCAATCC CCATATGGAA ACCGTCGATA TTCAGCCATG TGCCTTCTTC
  361 CGCGTGCAGC AGATGGCGAT GGCTGGTTTC CATCAGTTGC TGTTGACTGT AGCGGCTGAT
  421 GTTGAACTGG AAGTCGCCGC GCCACTGGTG TGGGCCATAA TTCAATTCGC GCGTCCCGCA
  481 GCGCAGACCG TTTTCGCTCG GGAAGACGTA CGGGGTATAC ATGTCTGACA ATGGCAGATC
  541 CCAGCGGTCA AAACAGGCGG CAGTAAGGCG GTCGGGATAG TTTTCTTGCG GCCCTAATCC
  601 GAGCCAGTTT ACCCGCTCTG CTACCTGCGC CAGCTGGCAG TTCAGGCCAA TCCGCGCCGG
  661 ATGCGGTGTA TCGCTCGCCA CTTCAACATC AACGGTAATC GCCATTTGAC CACTACCATC
  721 AATCCGGTAG GTTTTCCGGC TGATAAATAA GGTTTTCCCC TGATGCTGCC ACGCGTGAGC
  781 GGTCGTAATC AGCACCGCAT CAGCAAGTGT ATCTGCCGTG CACTGCAACA ACGCTGCTTC
  841 GGCCTGGTAA TGGCCCGCCG CCTTCCAGCG TTCGACCCAG GCGTTAGGGT CAATGCGGGT
  901 CGCTTCACTT ACGCCAATGT CGTTATCCAG CGGTGCACGG GTGAACTGAT CGCGCAGCGG
  961 CGTCAGCAGT TGTTTTTTTA TCGCCCAATC CACATCTGTG AAAGAAAGCC CTGACTGGGC
 1021 GGTTAAATTT GCCCAACGCT TATTACCCAG CTCGATGCAA AAATCCCATT TCGCTTGTTG
 1081 TTCAGATGCG GGATTGGCCA TGGACGCGCG GGGCAGCGTT CACACTGAGG ATTTTTCCCG
1141 CCAAGAACGG TCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles