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pLefty1p-luc (#RDB10004)

Oct-Sox enhancer carrying reporter derived from Lefty1 promoter.

Alternative name stock no 972
Clone info. Reporter construct with luciferase expression under the Lefty1 promoter.
Comment Genomic fragment was amplified by PCR with primers 5'- CCTTTACACTGGTCTCGAGCC-3' and 5'-ATAAGCTTGTCCGGGAAGAGGAGCCTTG-3'. Resulting fragment contains 1,379 bp from -1297 to +82 relative to the transcription start site of the Lefty1. See Fig.1 of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse Lefty1 genomic DNA
Depositor|Developer Niwa, Hitoshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB10004 pLefty1p-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pLefty1p-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10004).

Reference section:

Nakatake, Y., Fukui, N., Iwamatsu, Y., Masui, S., Takahashi, K., Yagi, R., Yagi, K., Miyazaki, J., Matoba, R., Ko, M.S., Niwa, H., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10004_A9C7p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3basic ()
Sequence file: RDB10004_A9C7a.seq check
>10004_10004_A9C7_3_pGL3basic_4406F_A01_01_ABI08.ab1
    1 GTTTTTGGCG GATTTCGGCC TATTGGTTAA AAATGAGCTG ATTTAACAAA AATTTAACGC
   61 GAATTTTAAC AAAATATTAA CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG
  121 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA
  181 AGTAAGTAAT ATTAAGGTAC GGGAGGTACT TGGAGCGGCC GCAATAAAAT ATCTTTATTT
  241 TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG TACTAACATA CGCTCTCCAT
  301 CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT
  361 GCCAGAACAT TTCTCTATCG ATAGGTACCG AGCTCTTACG CGTGCTAGCC CGGGCTCGAG
  421 CCAAGAAAGG CAGCGCATCG TGTCAGAAGC TGCAGACTTC ATTCCAGGGC CCCCCTCTCC
  481 TGGTTTGGGG CCTGCTTTCC AATCTCAAGC CTGACCTGGG TCAGACCTGG GCAGGAGCCA
  541 TGGGGAAGGC AGCTGCTCTT CTGCATAACA AAGGGTGGCC CTGCTGGAGA GCCAGGGAAC
  601 ACACCAGGGA CGAGTCTTAC AATCCACTAT GGTTTCTTCA TCTATCCCCC GACTAAGATT
  661 GTCTGAATCT GCAAATAAAA CCTCTAGATA CTGGTGTAAT GCAAATCCCA GGTAAACAGT
  721 GACTGATATA TTGTGTAACC AAGTCCCAGA TTCAGTACGG GTTTTATTTG GCAGCCCTAC
  781 CCCTGGCAGC TTGCTCAATT TTATTTCTCC AGCGCACAAA GCCACAAATT CACACTCGGG
  841 TATCCCAGCT GTGTCTTGGA GAAGGCTTAG CATATTACTG GTGTATAGCC ACACACCCTT
  901 GTCCCCACCC CAGCCACATG CCCCTGTCCC CAACCCAGCC ACACACCCTG TCCCCACCCC
  961 AGACACACAC CCCTGGGCAG GACTTAGCAG AAAATAATTG GAGCTGTGGG AAGCCCTTCG
 1021 TGGGCACCGA TCTTTAACTA TGTTGCCCCT CGTCTGAGCC GATGTACTCT GGCTCAGAGA
 1081 TTGGATGGGG TAGATTTTTC AATTCTTCCT TACAGCAGAA CGAAAGATAG TTTGCTCATT
 1141 GCGAGTCCAG CTTTGCAACT AGGAAGTCCC TCAGGCCTCT CAAGGTTGGG AGAAGCCCTT
1201 TCAACT
//
Primer: Luc_R ()
Sequence file: RDB10004_A9C7b.seq check
>10004_10004_A9C7_3_Luc_R_B01_02_ABI08.ab1
    1 TCATTGGTTC AGGGACCAGG GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG
   61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA
  121 TGGTGGCTTT ACCAACAGTA CCGGAATGCC AAGCTTGTCC GGGAAGAGGA GCCTTGGATG
  181 TGTGTGTCCC TGAGGTGTCC TGAAAGGGTC TTGAGTCTGC GGAGGAATGG TACGGTACGG
  241 AACAGCTTTA TAGCTGGTCC TGGGGTGGGG GGCAGGGAGG AAGAAGAGAG GTCACACCCC
  301 TGGGACCTCC TGGGAGCTCA GCATGAAACA AGCCCCTGAG TCCTGAGGAG GGGCTGTCTA
  361 GAAAGGACAC AAAAGCCTGT CTGGACTGAG AACATCTGGT CACCGCTGTC TGTTAGCCAT
  421 ATACTTCACC ATTGACTTAA GGGTGTCTGA CCAATTAGGA CATTCAGGAC AGGGGTGGTA
  481 ATAGCTCAGT GGTAGAGCAG CACCGGCCCT GCACACGTGA AGCCCTAAAT TCAATCCCAG
  541 CATGGGATGG AAGGTGGGTC CTGGAGACGA CGCGGAGGTC TCACGTTCTC TGCTGGATGC
  601 CTGCTCCCAG TGTCTGGAAT GTGCACCTGT AGTCTTGTCC ATCACTGCTT CTAAACCCTG
  661 GAGAGAACCA GATGTCAATC AACACCTGGG CTCTCCAACT GACTTGCTTC CCAGGTGTGC
  721 AAAAGACTTT TCTAGGGCTG ATAAGAGTGA GGCTTCTCCA CTTTGGGGGC TGGGGGACCT
  781 TCCTTGGTTG CAAAGCCTGG GACTCGCCAA TGAGCAACCT ATCCTTTCCC TTTCTTGCTG
  841 TAGGAGAATT GAAAATCTAC CCCATCCCAA TCTCTGAGCC AGAGTACATC GGCTCAGACG
  901 AGGGGCAACA TAGTTAAGAT CGGTGCCCAC GAAAGGGCTT TCCACAGCTC CAATTATTTT
  961 CTGCTAAGTC CTGCCCAGGG GTGTGTGTCT GGGGTGGGGA CAGGGTGTGT GGCTGGGTTT
 1021 GGGGACAGGG GCATGTGGCT GGGGTGGGGA CAAGGGTGTG TGGGCTATAC ACAGTAATAT
 1081 GCTAAGCCTT CTCCAAGACA CAGCTGGAAT ACCGAGTGTG AAGTTGTGCC TTTGTGCGCT
1141 GAGAATAATT GAGCAGCTGC CAAGTAGCTG ACAAGTGAAG CCTGGTATAA CG
//
Primer: pGCAP_R ()
Sequence file: RDB10004_A9C7c.seq check
>10004_10004_A9C7_3_pGCAP_R_C01_03_ABI08.ab1
    1 CAGTGAATCT TTATAATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTACACG
   61 GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC TGATTTTTCT TGCGTCGAGT
  121 TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA CTCCTCCGCG CAACTTTTTC
  181 GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT TTTCCGTCAT CGTCTTTCCG
  241 TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT CATCGTCGGG AAGACCTGCG
  301 ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG ATTCCAATTC AGCGGGAGCC
  361 ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT CAACGATGAA GAAGTGTTCG
  421 TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC CATCCTTGTC AATCAAGGCG
  481 TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA TAGGACCTCT CACACACAGT
  541 TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA GATCCACAAC CTTCGCTTCA
  601 AAAAATGGAA CAACTTTACC GACCGCGCCC GGTTTATCAT CCCCCTCGGG TGTAATCAGA
  661 ATAGCTGATG TAGTCTCAGT GAGCCCATAT CCTTGCCTGA TACCTGGCAG ATGGAACCTC
  721 TTGGCAACCG CTTCCCCGAC TTCCTTAGAG AGGGGAGCGC CACCAGAAGC AATTTCGTGT
  781 AAATTAGATA AATCGTATTT GTCAATCAGA GTGCTTTTGG CGAAGAAGGA GAATAGGGTT
  841 GGCACCAGCA GCGCACTTTG AATCTTGTAA TCCTGAAGGC TCCTCAGAAA CAGCTCTTCT
  901 TCAAATCTAT ACATTAAGAC GACTCGAAAT CCACATATCA AATATCCGAG TGTAGTAAAC
  961 ATTCCAAAAC CGTGATGGAA TGGAAACAAC ACTTAAAATC GCAGTATCCG GGAATGATTT
 1021 GATTGCCAAA AAATAGGATC TCTGGCATGC GAGAATCTCA CGCAAGGCAG TTCTATGAGG
 1081 CAGAGCGGAC CACCTTTTAG GCAAGACCAG TAGATCCAGA GGAGTTCAAT GGATCAGTGC
 1141 ATGTCTGGTC CCTAATCGGA AGACTCTGGG CACAAATTCG TATCCATTAA AAACCCGGGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]