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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBRblue-MC1DTApA (#RDB10550)

Expression clone of diphtheria toxin (DT-A)

Alternative name stock no 1555, pMC1DTApA
Clone info. Expression clone of diphtheria toxin (DT-A) under the control of MC1, polyoma enhancer and tk promoter.
Vector backbone pBluescript II KS (-) (Plasmid)
Gene/insert name Corynebacterium diphtheriae Diphtheria toxin A genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB10550z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する ()。
備考 タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB10550 pBRblue-MC1DTApA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBRblue-MC1DTApA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10550).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10550_A3Jap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: MC1 pro, DTA 5'
Sequence file: RDB10550_A3Jaa.seq check
>10550_10550_A9KcF_3_M13_-40_C08_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNNGNCTNN CTATAGGGCG AANTGGAGCT CCACCGCGGT
   61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATAT CAAGCTTATC
  121 GATACCGTCG AGCAGTGTGG TTTTCAAGAG GAAGCAAAAA GCCTCTCCAC CCAGGCCTGG
  181 AATGTTTCCA CCCAATGTCG AGCAGTGTGG TTTTGCAAGA GGAAGCAAAA AGCCTCTCCA
  241 CCCAGGCCTG GAATGTTTCC ACCCAATGTC GAGCAAACCC CGCCCAGCGT CTTGTCATTG
  301 GCGAATTCGA ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT
  361 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GGTCCTCGCC ATGGATCCTG
  421 ATGATGTTGT TGATTCTTCT AAATCTTTTG TGATGGAAAA CTTTTCTTCG TACCACGGGA
  481 CTAAACCTGG TTATGTAGAT TCCATTCAAA AAGGTATACA AAAGCCAAAA TCTGGTACAC
  541 AAGGAAATTA TGACGATGAT TGGAAAGGGT TTTATAGTAC CGACAATAAA TACGACGCTG
  601 CGGGATACTC TGTAGATAAT GAAAACCCGC TCTCTGGAAA AGCTGGAGGC GTGGTCAAAG
  661 TGACGTATCC AGGACTGACG AANGTTCTCG CACTAAAAGT GGATAATGCC GAAACTATTA
  721 AGAAAGAGTT AGGTTTAAGT CTCACTGAAC CGTTGATGGA GCAAGTCGGA ACGGAAGAGT
  781 TTATCAAAAG GNTCGGTGAT GGTGCTTCGC GTGTAGTGCT CAGCCTTCCC TTCGCTGANG
  841 GGAGTTCTNG CGTTGAATAT ATTANTNNNT GGGAACANGC GAAAGCGTTA ANCGTAGAAC
  901 NTGAGANTAA TTTTGAAATC CGNGGAAAAN GNNNCCNAGA TGCNATGTAT GAGTATATNG
  961 NNTNNANCCN GNGCNNNAAA TCNNGNNNNN NATNTNNTNN TGNANGGNAN NTTNNTNCTG
1021 NGGNGNGANN TNNNTNGNNN NANNNNNNNN NN
//
Primer: LTAlarge_upR (Pr0144)
Region: DTA
Sequence file: RDB10550_A3Jab.seq check
>10550_10550_A9KcF_3_LTAlarge_upR_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCA AACNATTAGN ANCAGTAGTT TAACACATTA TACACTTAAA
   61 AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA TTATGTCACA
  121 CCACAGAAGT AAGGTTCCTT CACAAAGAGA TCGCCTGACA CGATTTCCTG CACAGGCTTG
  181 AGCCATATAC TCATACATCG CATCTTGGCC ACGTTTTCCA CGGATTTCAA AATTAATCTC
  241 AAGTTCTACG CTTAACGCTT TCGCCTGTTC CCAGTTATTA ATATATTCAA CGCTAGAACT
  301 CCCCTCAGCG AAGGGAAGGC TGAGCACTAC ACGCGAAGCA CCATCACCGA ACCTTTTGAT
  361 AAACTCTTCC GTTCCGACTT GCTCCATCAA CGGTTCAGTG AGACTTAAAC CTAACTCTTT
  421 CTTAATAGTT TCGGCATTAT CCACTTTTAG TGCGAGAACC TTCGTCAGTC CTGGATACGT
  481 CACTTTGACC ACGCCTCCAG CTTTTCCAGA GAGCGGGTTT TCATTATCTA CAGAGTATCC
  541 CGCAGCGTCG TATTTATTGT CGGTACTATA AAACCCTTTC CAATCATCGT CATAATTTCC
  601 TTGTGTACCA GATTTTGGCT TTTGTATACC TTTTTGAATG GAATCTACAT AACCAGGTTT
  661 AGTCCCGTGG TACGAAGAAA AGTTTTCCAT CACAAAAGAT TTAGAAGAAT CAACAACATC
  721 ATCAGGATCC ATGGCGAGGA CCTGCAGGGT CGCTCGGTGT TCGANGCCAC ACGCGTCACC
  781 TTAATATGCG AANTGGANCT NNNANCGCGC CGCCCCGACN GCATCTGCGT GTTCGAATTC
  841 GCCAATGACA ANANGCTGGG CGGGGTTNGC TCGACATNNG GGNNGAAACA TTCCAGGCCN
  901 GGNNNGANNN GNTTTTTNGC TNCCNNTTGN NAAANCNCAC NGNNNGNNNN NNGNNNGNNN
961 NNTTCCNNNN CNNNNNNNNN N
//
Primer: reverse-2 (Pr0100)
Region: PGK p(A)
Sequence file: RDB10550_A3Jac.seq check
>10550_10550_A9KcF_3_reverse2_D08_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGCNN TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
   61 GTACCGGGCC CCCCCTCGAG GTCGGCCGCA TCTAGCTAGA GTCGATCGAC CAGCTTCTGA
  121 TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG TATGTGGAAC AGAGGCTGCT
  181 GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA CCACAGTACT TAGAACCACA
  241 AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT CTATAGATCT ATAGATCATG
  301 AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG TTTAAATTAT GATATCCAAC
  361 TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG TAAAGAGCAG GCATTTATCT
  421 AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC ATTCAAAATG TAGGTACTCT
  481 GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC CATTTTAGTG GACATCTTTA
  541 TTGTTTAATA GATCATCAAT TTCTGCATCC CTCGACTCTA GTGGATCTGC ATTCCACCAC
  601 TGCTCCCATT CATCAGTTCC ATAGGTTGGA ATCTAAAATA CACAAACAAT TAGAATCAGT
  661 AGTTTAACAC ATTATACACT TAAAAATTTT ATATTTACCT TAGAGCTTTA AATCTCTGTA
  721 GGTAGTTTGT CCAATTATGT CACACCACAG AAGTAANGTT CCTTCACAAA GAGATCGCCT
  781 GACACGATTT CCTGCACAGG CTTGAGCCAT ATACTCATAC ATCGCATCTN GGCCACGTTT
  841 TCCACGGATT TCAAAATTAA TCTCANGTTC TACGCTTAAC GCTTNCGNCN GTNNCNAGTT
  901 ATTAATATAN TCAACGCTAG AACTCCCNTC ANCGNANNGN NANGGNTGAN CNCTACNCGC
961 NNANCANCNN CACCGNANCN TTTTGANAAA CTCTTNCNGT NNCNANNTGN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles