Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-Klf5(2)-IP

Expression clone of mouse Klf5

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB10857_A8Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB10857_A8Eta.seq
>10857_10857_A8Et_4_pAxCA_F1_A08_02_ABI24.ab1     1 GGCTGCTACA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA    61 TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCCTCGAGAT CCAATCCCCA GACCGTTCAT   121 GCCCACGCGG GTGCTGACCA TGAGCGCCCG CCTGGGACCA CTGCCCCAGC CGCCGGCCGC   181 GCAGGACGAA CCCGTGTTCG CGCAGCTCAA GCCGGTGCTG GGCGCTGCGA ACCCGGCCCG   241 CGACGCGGCG CTCTTCTCCG GAGACGATCT GAAACACGCG CACCACCACC CGCCTGCGCC   301 GCCGCCAGCC GCTGGCCCGC GACTGCCCTC GGAGGAGCTG GTCCAGACAA GATGTGAAAT   361 GGAGAAGTAT CTGACCCCTC AGCTCCCTCC AGTTCCGATA ATTTCAGAGC ATAAAAAGTA   421 TAGACGAGAC AGTGCCTCAG TGGTAGACCA GTTCTTCACT GACACTGAAG GCATACCTTA   481 CAGCATCAAC ATGAACGTCT TCCTCCCTGA CATCACTCAC CTGAGAACTG GCCTCTACAA   541 ATCCCAGAGA CCATGCGTAA CACAGATCAA GACAGAACCT GTTACCATTT TCAGCCACCA   601 GAGCGAGTCG ACGGCCCCTC CTCCTCCTCC GGCCCCCACC CAGGCTCTCC CCGAGTTCAC   661 TAGTATCTTC AGCTCCCACC AGACCACAGC GCCACCACAG GAGGTGAACA ATATCTTCAT   721 CAAACAAGAA CTTTCCTAAT ACCAGATCTT CATCTCTCTG TCCCTTTCCC AGCAGGCCAC   781 CTGTACCAGC TGTTGAATAC ACCGGAATCT AGACATGCCC AGGTTCGAAC AAACCAGACG   841 GGCAGTTATT GGACACCCTT AAATGTTTTC TTATTGGGCA GGCTTAACCC AACACCCCCT   901 TCTGCTGTTC ACAGAACGTT CATGAAACAG TTCAAGGCAA TGCTCCCTTT GGCACGTACA   961 CCATGGCAAG TTCAGTTCTT CCACAAGCAG CACCTACTTC CCCGTACTGC CAGCCAGATC 1021 CTGAAAGTTC CTG //
Primer: CSII-CMV3' Sequence file: RDB10857_A8Etb.seq
>10857_10857_A8Et_4_CSII-CMV3'_F12_18_ABI24.ab1     1 CGGGGGTCAT TCGATATCAA GCTTATCGAG CGGCCCGCTC AGTTCTGGTG GCGCTTCATG    61 TGCAGCGCGA GGTGGTCGGA GCGGGAGAAG CTGCGTTGGC ACACCATGCA CTGGAACGGC   121 TTGGCGCCCG TGTGCTTCCT GTAGTGGCGG GTCAGCTCAT CCGACCGGGC AAACCTCCAG   181 TCGCAGCCCT CCCAGGTGCA CTTGTAGGGC TTCTCGCCCG TATGAGTCCT CAGGTGAGCT   241 TTTAAGTGAG ACGACTTTGT ATAAACTTTT GTGCAACCAT TATAATCGCA GAAGTGGATA   301 CGTCGCTTCT CCAGATCCGG GTTACTCCTT CTGTTGTATC TGACAGGTGG GAGAGTTGGC   361 GAATTAACTG GCAGAGTGGC AGGTAAATTT GGGTTGTGAA TCGCCAGTTT GGAAGCAATT   421 GTAGCGGCAT AGGACGGAGG TGGGGTGAGA TTCTGCAGCA TCTCAGCTTG TCTATCGGGA   481 CTTCCAGGCT CTGAGCTTGG TGGTGACGGG GGAAAGTAGG TGGCCTGCTG TGGAAGAAAC   541 TGACTTGGCA TGGTGTACGT GCAAGGGGGC ATGCCCTGGA ACTGTTTCAT TGACGTCTGT   601 GGAACAGCAG AGGGGTGTGG GTTAAGGCCT GCCATAGAAA CATTAAGGGT GTCCATTACT   661 GCCGTCTGGT TTGTCGAACT GGGCATGTCT AGATCCGGTG TATTCAACAG CTGGTACAGG   721 TGGCCCTGCT GGGAAGGGAC AGAGAGATGA AGATCTGGTA TAGGAAGTTC TTGTTTGATG   781 AAGATATTGT TCACCTCCTG TGGTGGCGCT GTGGTCTGGT GGGAGCTGAA GATACTAGTG   841 AACTCGGGGG AGAGCCTGGG TGGGGGGCCG GAGGAGGAGG AGGGGGCCGT CGACTCGCTC   901 TGGTGGCTGA AAATGGGTAA CAGGTTCTGT CTTGATCTGT GTTACGCATG GTCTCTGGGA   961 TTTGTAGAGG CCAGGTTCTC AGGTGAGTGA TGTTCAAGGA GGAGACGTTC ATGTTTGAAT  1021 GCTGTAATGT AATGCCCTTC CAGTGTCAGT GGAAGAACTT GGCTCTTACC CACTTGGGAG 1081 GGCCACCC //
Primer: CMV_en_R Sequence file: RDB10857_A8Etc.seq
>10857_10857_A8Et_4_CMV_en_R_C08_08_ABI24.ab1     1 TTTATTAATT GATTACTATT AATAACTAGC TAGTCAATAA TCAATGTCGA CGATGATATC    61 AGATCCAGAT ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG   121 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG   181 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG   241 TAATTTTCCT CCCGACTCTT AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA   301 ACTGACCGCA GCTGGCCGTG CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC   361 AGAGGGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT   421 CAGTGTGGTT TTGCAAGAGG AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC   481 CCAATCATTA CTATGACAAC AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGGCCCC   541 TGGCCTCCGC TTACTCTGGA GAAAAAGAAG AGAGGCTTCC AGAGGCAACT TGTCAAAACA   601 GGACTGGCGC CTTGGAGGCG CTGTGGGGCC ACCCAAATTG ATATAATTAA GCCCCAACCG   661 CCTCTTCCCG CCTCATTTCA GCCTCACACT ATGTGAAATA ATAGCTGAAT ACACAGTTTA   721 TTGAATAAAA CATTAATTTC CAGGAAAATA ACAAGTCTTT GTTTTTCCAA AGCGTCATTG   781 AAAAGAAAGA AGTATGAGTA TCAACATTCC GTGTCGCCTA ATCCTTTTTT GCGCAATTGC   841 CTCTGTTTGC TCACCAGAAC GCTGTGAAAG TTAAAAGAAT GCTGAAAGAA TACGATTGTG   901 CACAGATTGG TACATCGACT GAATTCCACA CGGTTAGAGT CTGGAAGTTC GCTCCGAAGA 961 A //
Primer: SV40pro_R Sequence file: RDB10857_A8Etd.seq
>10857_10857_A8Et_4_SV40pro_R_D08_11_ABI24.ab1     1 GCAATCGGAA AAACGGAGAA TGGGCGGAAC TGGGCGGAGT TAGCGCGGCG GGATGGGCGG    61 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC GGATCCGCTG GCACGACAGG   121 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT   181 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC   241 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGGGCTGCAG   301 GTTCTTTCCG CCTCAGAAGC CATAGAGCCC ACCGCATCCC CAGCATGCCT GCTATTGTCT   361 TCCCAATCCT CCCCCTTGCT GTCCTGCCCC ACCCCACCCC CCAGAATAGA ATGACACCTA   421 CTCAGACAAT GCGATGCAAT TTCCTCATTT TATTAGGAAA GGACAGTGGG AGTGGCACCT   481 TCCAGGGTCA AGGAAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC   541 ACAGTCGAGG CTGATCAGCG AGCTCTAGAT CATCGATGCA TGGGGTCGTG CGCTCCTTTC   601 GGTCGGGCGC TGCGGGTCGT GGGGGCGGGC GTCAGGCACC GGGGCTTGCG GGTCATGCAC   661 CAGGTGCGCG GGTCCTTTCG GGGCACCTCG ACGTCGGCGG GTGACGGTGA AACCCGAGCC   721 GCTCGTAGAA GGGGGAGGGT TTGCGGGGGG GCGGAAGGTC TCCAGGAAAA GGCGGGGCAC   781 CCCCGGCGCG CCTCGGCCCG CCCTCCCACT CCGGGGGGAA GCCCGACGGG CGCCTGCCCA 841 AAACCCTTGC CCCTGGGTGG GTCGGGGCGA AAACGCCTGA ACGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl