RDB12110 - pPyCAG-Gata6-IP

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pPyCAG-Gata6-IP (#RDB12110)

Expression vector of mouse Gata6.

Alternative name	stock no 20058
Clone info.	Expression vector of mouse Gata6. Please note that this clone contains partial fragment corresponding 694 to 2041 of NM_010258.3 (cds, 272..2041).
Comment	SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20. Known difference: C to T substitution (Ala443Val) at a nucleotide corresponding 2037nt of NM_010258.3.
Vector backbone	pPyCAG (Plasmid)
Gene/insert name	Mouse Gata6 cDNA
Depositor\|Developer	Niwa, Hitoshi \|

Sequence	RDB12110z.seq
Remarks, protocol and/or map (pdf)	RDB12110p.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12110	pPyCAG-Gata6-IP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-Gata6-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12110).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12110_A7Dep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB12110_A7Dea.seq
>12110_12110_A7De_2_pAxCA_F1_B02_02_ABI08.ab1 1 CCGTGACGGG CTACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC 61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCTA GTGATCGAGC AGCCGGAGGA 121 AATGTACCAG ACCCTCGCCG CCCTGTCCAG CCAGGGGCCC GCCGCTTACG ACGGCGCGCC 181 CGGCGGCTTC GTGCACTCCG CAGCGGCGGC GGCCGCTGCC GCCGCGGCAG CCAGCTCCCC 241 GGTCTACGTG CCCACCACGC GCGTGGGCTC CATGCTGTCC GGCCTGCCCT ACCTTCAAGG 301 GGCGGGCAGC GGGCCCAGCA ATCACGCGGG CGGAGCGGGT GCCCACCCAG GCTGGTCCCA 361 GGCCTCCGCC GACAGCCCCC CGTATGGCGG GGGTGGCGCA GCCGGCGGCG GCGCGGCCGG 421 ACCTGGAGGT GCGGGATCGG CTACGGCCCA CGCCTCTGCA CGCTTTCCCT ACTCGCCCAG 481 CCCGCCCATG GCCAACGGCG CCGCGCGAGA CCCCGGGGGC TACGTGGCTG CGGGCGGCAC 541 GGGCGCAGGC AGTGTGAGTG GAGGTGGCGG CAGCCTGGCG GCCATGGGTG GCCGGGAGCA 601 CCAGTACAGC TCGCTGTCCG CAGCTCGGCC GCTGAACGGA ACGTACCACC ACCACCATCA 661 CCATCACCCG ACCTACTCGC CCTACATGGC CGCACCGCTG ACTCCTGCCT GGCCAGCAGG 721 ACCCTTCGAA ACGCCGGTGC TCCACAGCTT ACAGGGCCGC GCGGGAGCTC CACTCCCGGT 781 GCCACGGGGC CCCAGCACAG ACCTGTTGGA GGACCTGTCG GAGAGCCGCG AGTGCGTGAA 841 CTGCGGCTCC ATCCAGACGC CACTGTGGAG ACGAGACGGG CACCGGTCAT TACCTGTGCA 901 ATGCATGCGG TCTCTACAGC AAGATGAATG GGCCTCAGCA GGCCCCTCAT CAAGCCCACA 961 GAAGCGGCGT GCCTTTCATC ACGGCGGCTT GGACTTGTTC CTTGTTGCCA ACTTGTTCAC 1021 ACCACACCAC TTACCTTTAT TGGCGTAGAA ATGGCTGAGG TGAGCCTGTG TTGCATGCTT 1081 GCCGGCTTCT ATTATATGAG CTTCTATGGG TTGACTTCGA CTCAC //
Primer: CSII-CMV3' Sequence file: RDB12110_A7Deb.seq
>12110_12110_A7De_2_CSII-CMV3'_D02_04_ABI08.ab1 1 CAAAGGAGAG AGGGGGGGGG GGCCGGAATT TGGCCGCGGG ATTTGGTAGC ACCAGCTCAG 61 ACCAGGGCCA GAGCACACCA AGAATCCTGT CGCACGGAGG ATGTGACTTC GGCAGGGGAG 121 GACAGACTGA CACCTATGTA GAGGCCGTCT TGACCTGAAT ACTTGAGGTC ACTGTTCTCG 181 GGGTTGGCGT TTTCTCCCAC TGCAGACATC ACTGATGCCC CTACCCCTGA GGTGGTCGCT 241 TGTGTAGAAG GAGAAGTATT TTTGGTGCAG TCATCTGAAT TAGAAGAAGA GGAAGTAGGA 301 GTCATAGGGA CAGAGCCACT GCTGTTACCG GAGCAAGCTT TTGACTTATT TATATTTTTA 361 GGTTTTCGTT TCCTGGTTTG AATTCCTTCT TTTTTCATAG CAAGTGGTCG AGGCACCCCA 421 TGGAGTTTCA TATAGAGCCC GCAAGCATTG CACACAGGCT CACCCTCAGC ATTTCTACGC 481 CATAAGGTAG TGGTTGTGGT GTGACAGTTG GCACAGGACA GTCCAAGCCG CCGTGATGAA 541 GGCACGCGCT TCTGTGGCTT GATGAGGGGC CTGCTGAGGC CATTCATCTT GCTGTAGAGA 601 CCGCATGCAT TGCACAGGTA ATGACCGGTG CCGTCTCGTC TCCACAGTGG CGTCTGGATG 661 GAGCCGCAGT TCACGCACTC GCGGCTCTCC GACAGGTCCT CCAACAGGTC TGTGCTGGGG 721 CCCCGTGGCA CCGGGAGTGG AGCTCCCGCG CGGCCCTGTA AGCTGTGGAG CACCGGCGTT 781 TCGAAGGGTC CTGCTGGCCA GGCAGGAGTC AGCGGTGCGG CCATGTAGGG CGAGTAGGTC 841 GGGTGATGGT GATGGGTGGT GGTG //
Primer: IRES-F Sequence file: RDB12110_A7Dec.seq
>12110_12110_A7De_2_IRES-F_F02_06_ABI08.ab1 1 AAATTGCTTT TAATGGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG 61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GACCGAGTAC 121 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC 181 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG 241 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG 301 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG 361 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC 421 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC 481 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG 541 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG 601 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG 661 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC 721 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGATGAT CTAGAGCTCG 781 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 841 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 901 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGGTG GGGCAGGAAC 961 AGCAAGGGGG AAGGAATGGG GAAGA //
Primer: Amp_R Sequence file: RDB12110_A7Ded.seq
>12110_12110_A7De_2_Amp_R_H02_08_ABI08.ab1 1 ATCCCTTTTC CAATGACCGG CTTTGGAAAA CAAAGACTGT ATTTCCTGGA AATTAATGTT 61 TATTCAATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG 121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC 181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG 241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG 301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA 361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC 421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG 481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA 541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT 601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 721 GATCTGATAT CATCGTCGAC ATTTGATTAT TGACTAGGTT ATTAATAGTA ATCAATTACG 781 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT TACATAACTT TACGGTAAAT 841 GGGCCCGGCC TGGCTGACCG CCCAAACGAC CCCCGCCCAT TTGACGTCAA TAATGACGTA 901 TGTTCCCATA GTAACGCCCA ATAGGGACTT TCCATTTGAC GTCAATGGGG TGGAGTATTT 961 ACGGTAACTT GCCCACTTTG GCAGTACATC AAGTGGTATC ATATGCCAAG TTACGCCTCC 1021 CCTTATTTGA CGTTCATGGA CGGTTAAAAT GGTCCGCCTT GCCCATATGT CCGGTTACAT 1081 GACTTAATGG AACTTTTCCC TACTGCAGTA CAACTTCTAT GACGGTA //