Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-cHA-IpacflexEGFP

Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker.

Catalog number RDB12114
Resource name pPyCAG-cHA-IpacflexEGFP
Clone info. Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker.
Vector backbone Plasmid
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence RDB12114z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12114 pPyCAG-cHA-IpacflexEGFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12114_A3E9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: IRES-F (Pr0036)
            Region: IRES,pacflexEGFP(PuroR 5')
            Sequence file: RDB12114_A3E9a.seq check
            >12114_12114_B0L2F_IRES-F_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNTNNNTCG NNGTNNNNNN ACGTCTAGGC CCCCCGAACC ACGGGGNCGT
               61 GGTTTTCCTT TGNAAAACAC GATGATAATA TGGCCACAAC CATGACCGAG TACAAGCCCA
              121 CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT
              181 TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA
              241 CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG
              301 CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG
              361 TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC
              421 AACAGATGGA AGGCCTCNNG NNGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA
              481 CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG
              541 GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA
              601 ACCTCNCCTT CTACNAGCGG CTCGGCTTCN CCGTCACCGC CNACGTCNAG GTGCCCGAAG
              661 GANNGCGNAC CTGGTGNATG ACCCGCNNGC CCNGNGNCNG GNTCGACNGN NNCNCGGGCC
            721 CNGGNNNCNN NNGGNNCNGG GGGNNNNNNN GNNNGGGGGN NNNNNNGNNN NNNNNNN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: bGHpA,PGK pA,pacflexEGFP(EGFP 3')
            Sequence file: RDB12114_A3E9b.seq check
            >12114_12114_B0L2F_bGH_rev3_C01_03_ABI08.ab1
                1 GNNNNNNNNN NNGNNNNNNN NNNGGGCNNA CAACAGNTGG CTGGCAACTA GAAGGCACAG
               61 TCGAGGCTGA TCAGCGAGCT CTAGATCATC GATAAGCTTA GGATCTATAG ATCATGAGTG
              121 GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA AATTATGATA TCCAACTATG
              181 AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA GAGCAGGCAT TTATCTAATC
              241 CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC AAAATGTAGG TACTCTGTTC
              301 TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATG TGGACATCTT TATTGTTTAA
              361 TAGATCATCA ATTTCTCGAC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC
              421 CACTATCGGC GAGTCGATAA GCTGGCCGCT TTACTTGTAC AGCTCGTCCA TGCCGAGAGT
              481 GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC
              541 TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC
              601 GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCNATGTT
              661 GTGGCGGNNC TTGAANTTCA CCTTGATGCC GTTCNTCTGC TNGTCGGCCA TGANATANAN
            721 NTTNNNGNNN GNTNNNNNNN NNN
            //
            Primer: PuroR_F (Pr0554)
            Region: pacflexEGFP(Flexible linker,EGFP 5')
            Sequence file: RDB12114_A3E9c.seq check
            >12114_12114_B0L2F_PuroR_F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNGCCC GNANGNNCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG
               61 CCGGATCGAC GGTACCGCGG GCCCGGGATC CACCGGTCGG GGGNTCAGGT GGCGGGGGAT
              121 CTGGTGGCGG ATCCAGCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
              181 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
              241 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC
              301 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC
              361 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
              421 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
              481 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
              541 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
              601 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCNA GATCCGCCAC AACATCNAGG
              661 NNGGCAGCGT GCAGCTCGNC NANCACTACC AGCNNNANCA CCCCCNNTCN GNCNNNNNCC
            721 CNNNNNNNNN N
            //
            Primer: EGFP-M_R (Pr0743)
            Region: pacflexEGFP(EGFP 5',Flexible linker)
            Sequence file: RDB12114_A3E9d.seq check
            >12114_12114_B0L2F_EGFP-M_R_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNGNGNNGG NNCTTGNNNT TNNCCTTGAT GCCGTTCTTC TGCTTGTCGG
               61 CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC
              121 CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA
              181 ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT
              241 GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT
              301 AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG
              361 GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT
              421 CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA
              481 TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGCTG GATCCGCCAC
              541 CAGATCCCCC GCCACCTGAT CCCCCGACCG GTGGNNCCCG GGCCCGCGGT ACCGTCGATC
              601 CGGCACCGGG CTTGCGGGTC ATGCACCANN TGCGCGGTCC TTCNGGCACC TCGACGTCNG
              661 CGGTGNNNGT GAANCCNANC CGCTCGTANN NNNGAGNTTG NNGGGNGNNN NNGNCTNCNN
            721 GNNNNNNGGN NNCCNNGNNN NNNNNNN
            //
            Primer: PGKpA_R (Pr0500)
            Region: PGK pA,pacflexEGFP(EGFP 3')
            Sequence file: RDB12114_A3E9e.seq check
            >12114_12114_B0L2F_PGKpA_R_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGN NNGNNNNNAC TTCCNTGTGG ACATCTTTAT TGTTTAATAG
               61 ATCATCAATT TCTCGACTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC
              121 TATCGGCGAG TCGATAAGCT GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
              181 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT
              241 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
              301 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG
              361 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT
              421 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
              481 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
              541 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GANGGTGCGC TCCTGGACGT AGCCTTCGGG
              601 CATGGCGGAC TTGAAGAANT CGTGCTGCTT CNTGTGNTCN GGGTAGCGGC TGAANCACTG
              661 CACGCCGTNN NTCNNNNNGG TCACGANGGN GGGNNNGGGN NNNGNNNNCN TNNNNNNNNG
            721 NNGNNNNNNN NNN
            //
            Primer: pCAG-F_V3 (Pr0495)
            Region: HA tag
            Sequence file: RDB12114_A3E9f.seq check
            >12114_12114_B0L2F_pCAG-F_V3_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNG GNANGGCGGG GTTCGGCTTC TGGCGTGTGA
               61 CCGGCGGCTC TANANCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
              121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCT
              181 CACGCGTGAC TAGTGCCATG GCCTACCCAT ACGACGTGCC AGACTACGCC TAGCGGCCGC
              241 TCGATAAGCT TGATATCGAA TTCCGCCCCC CCCCCCTCTC CNNNNNNNCC CCCTAACGTT
              301 ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC
              361 ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC
              421 ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCNTGAAG
              481 GAANNNNNTC CTCTGGAAGC TTCTTGAANA NNNNCAACGT CTGTAGCGAC CCTTTGCAGG
              541 CAGCGNANNC CCCCNNNTGN NNNNNGGTGC CTCTGCGGCC AAAANNCCNN NTGTATNANA
              601 TACACCNNCN NNNGCGGNNC NACCCCAGTG CCACGTTGNG ANNTNGNANN NTTGTGGNNN
              661 GNNNNAANNG GNTCTCNTCN AGCGNANTCA NNNNGGGGNT NNNNANGNNN NNNNGNANCC
            721 CNNNNNNNGG NNATNNGANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.04.10

            GNP_filter3_RDBDEP_html_230405.pl