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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-cHA-IpacflexEGFP (#RDB12114)

Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker.

Clone info. Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker.
Vector backbone Plasmid
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB12114z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12114 pPyCAG-cHA-IpacflexEGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-cHA-IpacflexEGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12114).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12114_A3E9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F (Pr0036)
Region: IRES,pacflexEGFP(PuroR 5')
Sequence file: RDB12114_A3E9a.seq check
>12114_12114_B0L2F_IRES-F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNTNNNTCG NNGTNNNNNN ACGTCTAGGC CCCCCGAACC ACGGGGNCGT
   61 GGTTTTCCTT TGNAAAACAC GATGATAATA TGGCCACAAC CATGACCGAG TACAAGCCCA
  121 CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT
  181 TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA
  241 CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG
  301 CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG
  361 TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC
  421 AACAGATGGA AGGCCTCNNG NNGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA
  481 CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG
  541 GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA
  601 ACCTCNCCTT CTACNAGCGG CTCGGCTTCN CCGTCACCGC CNACGTCNAG GTGCCCGAAG
  661 GANNGCGNAC CTGGTGNATG ACCCGCNNGC CCNGNGNCNG GNTCGACNGN NNCNCGGGCC
721 CNGGNNNCNN NNGGNNCNGG GGGNNNNNNN GNNNGGGGGN NNNNNNGNNN NNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,PGK pA,pacflexEGFP(EGFP 3')
Sequence file: RDB12114_A3E9b.seq check
>12114_12114_B0L2F_bGH_rev3_C01_03_ABI08.ab1
    1 GNNNNNNNNN NNGNNNNNNN NNNGGGCNNA CAACAGNTGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGATCATC GATAAGCTTA GGATCTATAG ATCATGAGTG
  121 GGAGGAATGA GCTGGCCCTT AATTTGGTTT TGCTTGTTTA AATTATGATA TCCAACTATG
  181 AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA GAGCAGGCAT TTATCTAATC
  241 CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC AAAATGTAGG TACTCTGTTC
  301 TCACCCTTCT TAACAAAGTA TGACAGGAAA AACTTCCATG TGGACATCTT TATTGTTTAA
  361 TAGATCATCA ATTTCTCGAC TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC
  421 CACTATCGGC GAGTCGATAA GCTGGCCGCT TTACTTGTAC AGCTCGTCCA TGCCGAGAGT
  481 GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC
  541 TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC
  601 GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCNATGTT
  661 GTGGCGGNNC TTGAANTTCA CCTTGATGCC GTTCNTCTGC TNGTCGGCCA TGANATANAN
721 NTTNNNGNNN GNTNNNNNNN NNN
//
Primer: PuroR_F (Pr0554)
Region: pacflexEGFP(Flexible linker,EGFP 5')
Sequence file: RDB12114_A3E9c.seq check
>12114_12114_B0L2F_PuroR_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGCCC GNANGNNCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG
   61 CCGGATCGAC GGTACCGCGG GCCCGGGATC CACCGGTCGG GGGNTCAGGT GGCGGGGGAT
  121 CTGGTGGCGG ATCCAGCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
  181 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
  241 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC
  301 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC
  361 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
  421 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
  481 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
  541 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
  601 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCNA GATCCGCCAC AACATCNAGG
  661 NNGGCAGCGT GCAGCTCGNC NANCACTACC AGCNNNANCA CCCCCNNTCN GNCNNNNNCC
721 CNNNNNNNNN N
//
Primer: EGFP-M_R (Pr0743)
Region: pacflexEGFP(EGFP 5',Flexible linker)
Sequence file: RDB12114_A3E9d.seq check
>12114_12114_B0L2F_EGFP-M_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNGNGNNGG NNCTTGNNNT TNNCCTTGAT GCCGTTCTTC TGCTTGTCGG
   61 CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC
  121 CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA
  181 ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT GAAGAAGATG GTGCGCTCCT
  241 GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG TGGTCGGGGT
  301 AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC GAGGGTGGGC CAGGGCACGG
  361 GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT GCCGTAGGTG GCATCGCCCT
  421 CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC TCGACCAGGA
  481 TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGCTG GATCCGCCAC
  541 CAGATCCCCC GCCACCTGAT CCCCCGACCG GTGGNNCCCG GGCCCGCGGT ACCGTCGATC
  601 CGGCACCGGG CTTGCGGGTC ATGCACCANN TGCGCGGTCC TTCNGGCACC TCGACGTCNG
  661 CGGTGNNNGT GAANCCNANC CGCTCGTANN NNNGAGNTTG NNGGGNGNNN NNGNCTNCNN
721 GNNNNNNGGN NNCCNNGNNN NNNNNNN
//
Primer: PGKpA_R (Pr0500)
Region: PGK pA,pacflexEGFP(EGFP 3')
Sequence file: RDB12114_A3E9e.seq check
>12114_12114_B0L2F_PGKpA_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNGNNNNNAC TTCCNTGTGG ACATCTTTAT TGTTTAATAG
   61 ATCATCAATT TCTCGACTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC
  121 TATCGGCGAG TCGATAAGCT GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
  181 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT
  241 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
  301 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG
  361 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT
  421 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
  481 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
  541 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GANGGTGCGC TCCTGGACGT AGCCTTCGGG
  601 CATGGCGGAC TTGAAGAANT CGTGCTGCTT CNTGTGNTCN GGGTAGCGGC TGAANCACTG
  661 CACGCCGTNN NTCNNNNNGG TCACGANGGN GGGNNNGGGN NNNGNNNNCN TNNNNNNNNG
721 NNGNNNNNNN NNN
//
Primer: pCAG-F_V3 (Pr0495)
Region: HA tag
Sequence file: RDB12114_A3E9f.seq check
>12114_12114_B0L2F_pCAG-F_V3_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNG GNANGGCGGG GTTCGGCTTC TGGCGTGTGA
   61 CCGGCGGCTC TANANCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
  121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCT
  181 CACGCGTGAC TAGTGCCATG GCCTACCCAT ACGACGTGCC AGACTACGCC TAGCGGCCGC
  241 TCGATAAGCT TGATATCGAA TTCCGCCCCC CCCCCCTCTC CNNNNNNNCC CCCTAACGTT
  301 ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC
  361 ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC
  421 ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCNTGAAG
  481 GAANNNNNTC CTCTGGAAGC TTCTTGAANA NNNNCAACGT CTGTAGCGAC CCTTTGCAGG
  541 CAGCGNANNC CCCCNNNTGN NNNNNGGTGC CTCTGCGGCC AAAANNCCNN NTGTATNANA
  601 TACACCNNCN NNNGCGGNNC NACCCCAGTG CCACGTTGNG ANNTNGNANN NTTGTGGNNN
  661 GNNNNAANNG GNTCTCNTCN AGCGNANTCA NNNNGGGGNT NNNNANGNNN NNNNGNANCC
721 CNNNNNNNGG NNATNNGANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles