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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPthc-Oct3/4 (#RDB12115)

Exchange vector to introduce Oct3 into the ROSA locus

Alternative name stock no 10219
Clone info. Exchange vector to introduce Oct3 into the ROSA locus.
Comment See Figures in Masui et al., Nucleic Acids Res. 33: e4 (2005).
Vector backbone pBluescript II KS (-) (Plasmid)
Gene/insert name Mouse Oct3/4 cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB12115z.seq
Additional map

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nucleic Acids Res. 33: e4 (2005).).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nucleic Acids Res. 33: e4 (2005))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12115 pPthc-Oct3/4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPthc-Oct3/4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12115).

Reference section:

Masui, S., Shimosato, D., Toyooka, Y., Yagi, R., Takahashi, K., Niwa, H., An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit. Nucleic Acids Res. 33 (4): e43 (2005). PMID 15741176. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12115_A5Ee.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMVx_F
Sequence file: RDB12115_A5Eea.seq check
>12115_12115_A5Ee_CMVx_F_A02_02.ab1
    1 AGGGGAGTCC AAACCGGGCC CTCTGCTAAC CATGTTCATG CCTTCTTCTC TTTCCTACAG
   61 CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG
  121 GTCAATTCCG TGAGCCGTCT TTCCACCAGG CCCCCGGCTC GGGGTGCCCA CCTTCCCCAT
  181 GGCTGGACAC CTGGCTTCAG ACTTCGCCTT CTCACCCCCA CCAGGTGGGG GTGATGGGTC
  241 AGCAGGGCTG GAGCCGGGCT GGGTGGATTC TCGAACCTGG CTAAGCTTCC AAGGGCCTCC
  301 AGGTGGGCCT GGAATCGGAC CAGGCTCAGA GGTATTGGGG ATCTCCCCAT GTCCGCCCGC
  361 ATACGAGTTC TGCGGAGGGA TGGCATACTG TGGACCTCAG GTTGGACTGG GCCTAGTCCC
  421 CCAAGTTGGC GTGGAGACTT TGCAGCCTGA GGGCCAGGCA GGAGCACGAG TGGAAAGCAA
  481 CTCAGAGGGA ACCTCCTCTG AGCCCTGTGC CGACCGCCCC AATGCCGTGA AGTTGGAGAA
  541 GGTGGAACCA ACTCCCGAGG AGTCCCAGGA CATGAAAGCC CTGCAGAAGG AGCTAGAACA
  601 GTTTGCCAAG CTGCTGAAGC AGAAGAGGAT CACCTTGGGG TACACCCAGG CCGACGTGGG
  661 GCTCACCCTG GGCGTTCTCT TTGGAAAGGT GTTCAGCCAG ACCACCATCT GTCGCTTCGA
  721 GGCCTTGCAG CTCAGCCTTA AGAACATGTG TAAGCTGCGG CCCCTGCTGG AGAAGTGGGT
  781 GGAGGAAGCC GACAACAATG AGAACCTTCA GGAGATATGC AAATCGGAGA CCCTGGTGCA
  841 GGCCCGGAAG AGAAAGCGAA CTAGCATTGA GAACCGTGTG AGGTGGAGTC TGGAGACCAT
  901 GTTTCTGAAG TGCCCGAAGC CCTCCCTACA GCAGATCACT CACATCGCCA ATCAGCTTGG
  961 GCTAGAGAAG GATGTGGTTC GAGTATGGTT CTGTAACCGG CGCCAGAAGG CAAAAGATCA
 1021 AGTATTGAGT ATTCCCAACG AGAGAGTATG AGGCTACAGG GACACCTTTC CCAGGGGGGG
 1081 GCTGTATCTT CTCTGCCCCC AAGGTCCCCA CTTTTGCACC CAGCTATGAG GCCCCACTTC
1141 ACCACACTCT ACTCAGTCCC CTTTTTTCCT GGAA
//
Primer: M13_-40
Sequence file: RDB12115_A5Eeb.seq check
>12115_12115_A5Ee_M13(-40)_B02_05.ab1
    1 CAGCCCTTAG CGCCGCGTAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC ACCGCCGGCA
   61 GACGCGTATA ACTTCGTATA GGATACTTTA TACGAAGTTA TGCGGCCGCT CTAGAACTAG
  121 TGGATCCCCC AATTCCCCAG TCTTTATTTA AGAACAAAAT GATGAGTGAC AGACAGGCCA
  181 GGCTCCTGAT CAACAGCATC ACTGAGCTTC TTTCCCCATC CCACCCCCCA CCCCTGTTGT
  241 GCTTTTAATC CCTCCTCAGT AAAAGAATTT AACCCCAAAG CTCCAGGTTC TCTTGTCTAC
  301 CTCCCTTGCC TTGGCTCACA GCATCCCCAG GGAGGGCTGG TGCCTCAGTT TGAATGCATG
  361 GGAGAGCCCA GAGCAGTGAC GGGAACAGAG GGAAAGGCCT CGCCCTCAGG AAAAGGGACT
  421 GAGTAGAGTG TGGTGAAGTG GGGGCTTCCA TAGCCTGGGG TGCCAAAGTG GGGACCTGGG
  481 GGCAGAGGAA AGGATACAGC CCCCCCTGGG AAAGGTGTCC CTGTAGCCTC ATACTCTTCT
  541 CGTTGGGAAT ACTCAATACT TGATCTTTTG CCCTTCTGGC GCCGGTTACA GAACCATACT
  601 CGAACCACAT CCTTCTCTAG CCCAAGCTGA TTGGCGATGT GAGTGATCTG CTGTAGGGAG
  661 GGCTTCGGGC ACTTCAGAAA CATGGTCTCC AGACTCCACC TCACACGGTT CTCAATGCTA
  721 GTTCGCTTTC TCTTCCGGGC CTGCACCAGG GTCTCCGATT TGCATATCTC CTGAAGGTTC
  781 TCATTGTTGT CGGCTTCCTC CACCCACTTC TCCAGCAGGG GCCGCAGCTT ACACATGTTC
  841 TTAAGGCTGA GCTGCAAGGC CTCGAAGCGA CAGATGGTGG TCTGGCTGAA CACCTTTCCA
  901 AAGAGAACGC CCAGGGTGAG CCCCACGTCG GCCTGGGTGT ACCCCAAGGT GATCCTCTTC
  961 TGCTTCAGCA GCTTGGCAAA CTGTTCTAGC TCCTTCTGCA GGGCTTTCAT GTCCTGGGAC
 1021 TCCTCGGGAG TTGGGTTCCA CCTTTCTCCA ACTTCCACGG CATTGGGGGC GGTCGGCACA
 1081 GGCTCAGAGG AGGTTTCCCT CCTGAGTGCT TCACTCGTGC TCCTGCCCTG GGTCCTCAGC
 1141 TGCAAGTCCT CCACGCCCAA CCTGGGGGAC TAGCCAAGTC CATCCTGGAG GTCCCACACA
1201 GTAA
//
Primer: PGKpro_F
Sequence file: RDB12115_A5Eec.seq check
>12115_12115_A5Ee_PGKpro_F_E02_14.ab1
    1 ATCTCCCCCG GGTTCCTCTC TTCCTCATCT CCGGGCCTTT CGACCGATCA TCAAGCTTGA
   61 TCCTCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG
  121 CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC
  181 CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC
  241 TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC
  301 CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA
  361 GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA
  421 AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC
  481 TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT
  541 TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA
  601 CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG
  661 CCGGATCCAT GCCCACGCTA CTGCGGGTTT ATATAGACGG TCCCCACGGG ATGGGGAAAA
  721 CCACCACCAC GCAACTGCTG GTGGCCCTGG GTTCGCGCGA CGATATCGTC TACGTACCCG
  781 AGCCGATGAC TTACTGGCGG GTGCTGGGGG CTTCCGAGAC AAATCGCGAA CATCTACACC
  841 ACACAACACC GCCTCGACCA GGGTGAGATA TCGGCCGGGG GACGCGGCGG TGGTAATGAC
  901 AAGCGCCCAG ATAACAATGG GCATGCCTTA TGCCGTGACC GACGCCGTTC TGGCTCCTCA
  961 TATCGGGGGG GAGGCTGGGA GCTCACATGC CCCGCCCCCG GGCCCTCACC CTCATCTTCG
 1021 ACCGCCATCC ATCGCCGCCC TCCTGATGCT ACCCGGGCCG CGCGGGTACC CTTATGGGCA
 1081 GCATGAACCC CCCAAGCCGT GCTGGCGTTC GTGGGTCCTC ATTCCGGCGG AACCTGGCTG
1141 CACAACCATC GTGCTTGGGG ATCATTCGGG GAAAGGACAG AGAACACACA TTTC
//
Primer: BGH_rev
Sequence file: RDB12115_A5Eed.seq check
>12115_12115_A5Ee_BGH_rev_C02_08.ab1
    1 GACCAGAACA TCGGATACCG GCGACACCTG GCCGCTCTAG AGCTCAGTTA GCCTCCCCCA
   61 TCTCCCGGGC AAACGTGCGC GCCAGGTCGC ATATCGTCGG TATGGAGCCG GGGGTGGTGA
  121 CGTGGGTCTG GACCATCCCG GAGGTAAGTT GCAGCAGGGC GTCCCGGCAG CCGGCGGGCG
  181 ATTGGTCGTA ATCCAGGATA AAGACGTGCA TGGAACGGAG GCGTTTGGCC AAGACGTCCA
  241 AGGCCCAGGC AAACACGTTA TACAGGTCGC CGTTGGGGGC CAGCAACTCG GGGGCCCGAA
  301 ACAGGGTAAA TAACGTGTCC CCGATATGGG GTCGTGGGCC CGCGTTGCTC TGGGGCTCGG
  361 CACCCTGGGG CGGCACGGCC GTCCCCGAAA GCTGTCCCCA GTCCTCCCGC CACGACCCGC
  421 CGCACTGCAG ATACCGCACC GTATTGGCAA GTAGCCCGTA AACGCGGCGA ATCGCAGCCA
  481 GCATAGCCAG GTCCAGCCGC TCGCCGGGGC GCTGGCGTTT GGCCAGGCGG TCGATGTGTC
  541 TGTCCTCCGG AAGGGCCCCA AGCACGATGT TGGTGCCGGG CAAGGTCGGC GGGATGAGGG
  601 CCACGAACGC CAGCACGGCC TGGGGGGTCA TGCTGCCCAT AAGGTACCGC GCGGCCGGGT
  661 AGCACAGGAG GGCGGCGATG GGATGGCGGT CGAAGATGAG GGTGAGGGCC GGGGGCGGGG
  721 CATGTGAGCT CCCAGCCTCC CCCCCGATAT GAGGAGCCAG AACGGCGTCG GTCACGGCAT
  781 AAGGCATGCC CATTGTTATC TGGGCGCTTG TCATTACCAC CGCCGCGTCC CCGGCCGATA
  841 TCTCACCCTG GTCGAGGCGG TGTTGTGTGG TGTAGATGTT CGCGATTGTC TCGGAAGCCC
  901 CCAGCACCCG CCAGTAAGTC ATCGGCTCGG GTACGTAGAC GATATCGTCG CGCGAACCCA
  961 GGGCCACCAG CAGTTGCGTG GTGGTGGTTT TCCCCATCCC GTGGGGACCG TCTATATAAC
 1021 CCGCAGTAGC GTGGGCATGG AATCCGGCAC CGGGCTTGCG GGTCATGCAC CAGTTGCGCG
 1081 GTCCTTTCGG CACCTCGAAC GTCGGCGTGA CGGTGAAAGC CGGAGCCGCC TCGGTAGAGG
1141 GAGGTTGGCG GGGCCGCGAG GTCTTCCAAG GAGCGGCACT CCTGGCGGGC GACTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Masui, S., An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit. Nucleic Acids Res. 33 (4): e43 (2005). PMID 15741176. [link to RRC of NBRP]