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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-mRFP1-RfA (#RDB12128)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.

Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB12128z.seq
Remarks, protocol and/or map (pdf) RDB12128.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12128 CSII-EF-mRFP1-RfA Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-mRFP1-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12128).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12128_A3Eop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1 alpha pro,mRFP1 5'
Sequence file: RDB12128_A3Eoa.seq check
>12128_12128_B1CoF_EF1a_C_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNTTTNANT TTGGNNCTTG GTTNNTTCTN NAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGCTAC CGGTCTCGAC TGGATCCGAT
  121 GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC
  181 CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC
  241 CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT
  301 GTCCCCTCAG TTCCAGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA
  361 CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA
  421 CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA
  481 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT
  541 GGGCTGGGAG GCCTCCACCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA ANGNCGAGAT
  601 CNAGATGANG NTGAAGCTGA AGGANGGCNG CCACTACGAC GCCNANGTCN AGANCACCTA
  661 CATGGNCNAN AAGCCCGTGN NGCTGNCNNG CNCNTANANA NNGANNTCNN GCNGGNNNTC
721 NNNNCNNNNN NNNNN
//
Primer: Cm_N_rev (Pr0014)
Region: RfA(CmR,attR1),PRE
Sequence file: RDB12128_A3Eob.seq check
>12128_12128_B1CoF_Cm_N_rev_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANGCNN TTGGGNNATA TCAACNNGTG GTATATCCNG
   61 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGACGGA TCCTAACTCA
  121 AAATCCACAC ATTATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGCGG
  181 CCGCCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT TTTTATGCAA
  241 AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG CTTTTTTGTA
  301 CAAACTTGTG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC CCCCCTTTTC
  361 TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA AAGGTACCTG
  421 AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG CACAATCAGC
  481 ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT CTCGACGGTA
  541 TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGANGGCGAA NGCGAANANN
  601 CGGAANANGN CGCANAGCCN GCAGCANGCC GCGGGAANGA ANGNCCNCTG GATTGANGGN
  661 CNAANGNANN TANCNNANNN NNNCCNNNGC ANAATCNANG NGGNNNNNCN NNCNANNNNN
721 CNNNNNANGN NNNNNNCNNN NNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,3' LTR,RfA(attR2,ccdB)
Sequence file: RDB12128_A3Eoc.seq check
>12128_12128_B1CoF_bGH_rev3_G01_07_ABI08.ab1
    1 NNNNNNNNNN NGNNNGGNGG NNGGGCANAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG
  121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA
  181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC
  241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATCACCA CTTTGTACAA
  301 GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA
  361 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGTCGACCTG
  421 CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC AGGTTAATGG
  481 CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG ATAACGGAGA
  541 CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG ATATGCACCA
  601 CCGGGTAAAG TTCACGGGAG ANTTTATCTG ACAGCANANG TGCACNGGCC NGGGGGNNNA
  661 CCATCCGTCG CCCGGNNNNN NCNNTNATAT CNCTNNGNAC NNNCNNNANN NGACNANNNN
721 NNNNNN
//
Primer: HIV-B_R (Pr0673)
Region: HIV-1 psi,5' LTR,CMV pro,CMV en
Sequence file: RDB12128_A3Eod.seq check
>12128_12128_B1CoF_HIV-B_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT
   61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC
  121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
  181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
  241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
  301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC
  361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
  421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA
  481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC
  541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT
  601 AANGTCATGT ACTNNNCATA ATGCCNGGCG GGCCATTTAN CGTCNTTGAC GTCNNNANGG
  661 GGCGNANTTN GNNNATGATA CACTNGNTGT ACTGNCNNNN GGGNANNTNA NNNNNANNNN
721 NNNNCNNNNN NNNNN
//
Primer: RRE_F1 (Pr0796)
Region: RRE
Sequence file: RDB12128_A3Eoe.seq check
>12128_12128_B1CoF_RRE_F1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNANNNTTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT
   61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
  121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
  181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
  241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
  301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
  361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
  421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
  481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
  541 GGGACCCGAC AGGCCCGAAN GAATAGAAGA AGAANGTGGA GAGAGAGACA GAGACNGATC
  601 CATTCGATTA GTGAACGGAT CTACNAATGG CAGTATTCAT CCNCAATTTT AAAAGAAAAG
  661 GGGGGNNTGG GGGGNACNGT GCNNGGNAAA GANANNANAN NTAATANCAN NNANNTNCNA
721 NNNAAGNNNN NAANNNNNNN NNN
//
Primer: cPPT/CTS-E_R (Pr0725)
Region: cPPT_CTS
Sequence file: RDB12128_A3Eof.seq check
>12128_12128_B1CoF_cPPT_CTS-E_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNTTTGNA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
   61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
  121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
  181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
  241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
  301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
  361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
  421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
  481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
  541 ACTCCNACTA GCATTCCNAG GCACAGCAGT GGTGCAAATG AGTTTTCCNG AGCAACCCCA
  601 AATCCCCAGG AGCTGTTGAT CCTTTANGTA TCTTTCCNCA GCCNGGANTC TTGNCNGGAN
  661 CTGCTTGATG CCCCNNACNG NGANTTGCNN CNNANGCNGT NGCGCCTNNN NNNNCNNCNN
721 CNANNNNNNN GCNGCNNNNN NNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,mRFP1 3'
Sequence file: RDB12128_A3Eog.seq check
>12128_12128_B1CoF_PRE_rev_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNGNNNNN NAGCAACNTA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGAT
  121 CCGCGGCCGC GAATTCTTAG GCGCCGGTGG AGTGGCGGCC CTCGGCGCGC TCGTACTGTT
  181 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAG CTTGATGTCG GTCTTGTAGG
  241 CGCCGGGCAG CTGCACGGGC TTCTTGGCCA TGTAGGTGGT CTTGACCTCG GCGTCGTAGT
  301 GGCCGCCGTC CTTCAGCTTC AGCCTCATCT TGATCTCGCC CTTCAGGGCG CCGTCCTCGG
  361 GGTACATCCG CTCGGTGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT
  421 CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG TCCTGCAGGG
  481 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT
  541 TGAAGCCCTC GGGGAANGAC AGCTTCNAGT AGTCGGGGAT GTCGGCGGGG TGCTTCACGT
  601 ANGCCTTGGA GCCGTACTGG AACTGANGGG NNAGGANGTC CNANGCGAAN GGNAGGGGGC
  661 CGCCCTNGNN CNNCTTNNGC TNNNNNGNCT GGNNGNCCNC NNNNGGNCGG NCNNNNNCNT
721 CNNNNNNNTN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Ishibashi, R., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. [link to RRC of NBRP]
user_report Ikeda, M., Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-Dependent Manner. Mol. Cell. Biol. 37 (5). pii: e00417-16 (2016). PMID 27956699. [link to RRC of NBRP]