Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-mRFP1-RfA

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12128_A3Eop1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1 alpha pro,mRFP1 5' Sequence file: RDB12128_A3Eoa.seq
>12128_12128_B1CoF_EF1a_C_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNTTTNANT TTGGNNCTTG GTTNNTTCTN NAGCCTCAGA CAGTGGTTCA    61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGCTAC CGGTCTCGAC TGGATCCGAT   121 GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC   181 CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC   241 CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT   301 GTCCCCTCAG TTCCAGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA   361 CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA   421 CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA   481 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT   541 GGGCTGGGAG GCCTCCACCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA ANGNCGAGAT   601 CNAGATGANG NTGAAGCTGA AGGANGGCNG CCACTACGAC GCCNANGTCN AGANCACCTA   661 CATGGNCNAN AAGCCCGTGN NGCTGNCNNG CNCNTANANA NNGANNTCNN GCNGGNNNTC 721 NNNNCNNNNN NNNNN //
Primer: Cm_N_rev (Pr0014) Region: RfA(CmR,attR1),PRE Sequence file: RDB12128_A3Eob.seq
>12128_12128_B1CoF_Cm_N_rev_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNANGCNN TTGGGNNATA TCAACNNGTG GTATATCCNG    61 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGACGGA TCCTAACTCA   121 AAATCCACAC ATTATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGCGG   181 CCGCCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT TTTTATGCAA   241 AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG CTTTTTTGTA   301 CAAACTTGTG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC CCCCCTTTTC   361 TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA AAGGTACCTG   421 AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG CACAATCAGC   481 ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT CTCGACGGTA   541 TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGANGGCGAA NGCGAANANN   601 CGGAANANGN CGCANAGCCN GCAGCANGCC GCGGGAANGA ANGNCCNCTG GATTGANGGN   661 CNAANGNANN TANCNNANNN NNNCCNNNGC ANAATCNANG NGGNNNNNCN NNCNANNNNN 721 CNNNNNANGN NNNNNNCNNN NNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGHpA,3' LTR,RfA(attR2,ccdB) Sequence file: RDB12128_A3Eoc.seq
>12128_12128_B1CoF_bGH_rev3_G01_07_ABI08.ab1     1 NNNNNNNNNN NGNNNGGNGG NNGGGCANAC AACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCCACACT GACTAAAAGG   121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA   181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC   241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATCACCA CTTTGTACAA   301 GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA   361 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGTCGACCTG   421 CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC AGGTTAATGG   481 CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG ATAACGGAGA   541 CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG ATATGCACCA   601 CCGGGTAAAG TTCACGGGAG ANTTTATCTG ACAGCANANG TGCACNGGCC NGGGGGNNNA   661 CCATCCGTCG CCCGGNNNNN NCNNTNATAT CNCTNNGNAC NNNCNNNANN NGACNANNNN 721 NNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi,5' LTR,CMV pro,CMV en Sequence file: RDB12128_A3Eod.seq
>12128_12128_B1CoF_HIV-B_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT    61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC   121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC   181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC   241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG   301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC   361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT   421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA   481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC   541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT   601 AANGTCATGT ACTNNNCATA ATGCCNGGCG GGCCATTTAN CGTCNTTGAC GTCNNNANGG   661 GGCGNANTTN GNNNATGATA CACTNGNTGT ACTGNCNNNN GGGNANNTNA NNNNNANNNN 721 NNNNCNNNNN NNNNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB12128_A3Eoe.seq
>12128_12128_B1CoF_RRE_F1_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNGNNNNNN NNNANNNTTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT    61 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG   121 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC   181 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA   241 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT   301 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA   361 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT   421 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT   481 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG   541 GGGACCCGAC AGGCCCGAAN GAATAGAAGA AGAANGTGGA GAGAGAGACA GAGACNGATC   601 CATTCGATTA GTGAACGGAT CTACNAATGG CAGTATTCAT CCNCAATTTT AAAAGAAAAG   661 GGGGGNNTGG GGGGNACNGT GCNNGGNAAA GANANNANAN NTAATANCAN NNANNTNCNA 721 NNNAAGNNNN NAANNNNNNN NNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS Sequence file: RDB12128_A3Eof.seq
>12128_12128_B1CoF_cPPT_CTS-E_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNTTTGNA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT    61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT   121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC   181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA   241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT   301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT   361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT   421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT   481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT   541 ACTCCNACTA GCATTCCNAG GCACAGCAGT GGTGCAAATG AGTTTTCCNG AGCAACCCCA   601 AATCCCCAGG AGCTGTTGAT CCTTTANGTA TCTTTCCNCA GCCNGGANTC TTGNCNGGAN   661 CTGCTTGATG CCCCNNACNG NGANTTGCNN CNNANGCNGT NGCGCCTNNN NNNNCNNCNN 721 CNANNNNNNN GCNGCNNNNN NNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,mRFP1 3' Sequence file: RDB12128_A3Eog.seq
>12128_12128_B1CoF_PRE_rev_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNGNNNNN NAGCAACNTA GTTAAGAATA CCAGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGAT   121 CCGCGGCCGC GAATTCTTAG GCGCCGGTGG AGTGGCGGCC CTCGGCGCGC TCGTACTGTT   181 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAG CTTGATGTCG GTCTTGTAGG   241 CGCCGGGCAG CTGCACGGGC TTCTTGGCCA TGTAGGTGGT CTTGACCTCG GCGTCGTAGT   301 GGCCGCCGTC CTTCAGCTTC AGCCTCATCT TGATCTCGCC CTTCAGGGCG CCGTCCTCGG   361 GGTACATCCG CTCGGTGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT   421 CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG TCCTGCAGGG   481 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT   541 TGAAGCCCTC GGGGAANGAC AGCTTCNAGT AGTCGGGGAT GTCGGCGGGG TGCTTCACGT   601 ANGCCTTGGA GCCGTACTGG AACTGANGGG NNAGGANGTC CNANGCGAAN GGNAGGGGGC   661 CGCCCTNGNN CNNCTTNNGC TNNNNNGNCT GGNNGNCCNC NNNNGGNCGG NCNNNNNCNT 721 CNNNNNNNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl