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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMYs-Tead2-EnR-IRES-EGFP (#RDB12175)

Retroviral vector of mouse Tead2, dominant negative

Clone info. Retroviral vector of mouse Tead2, Engrailed repression domain, dominant negative.
Comment C-terminal Myc-tag. Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA Drosophila engrailed cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12175 pMYs-Tead2-EnR-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-Tead2-EnR-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12175).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19900_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(SV40 NLS,Tead2 1-127aa,EnR 5')
Sequence file: RDB19900_B3Baa.seq check
>12175_19900_B3Ba_3_MMLV-pol_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGNNCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCGC
  121 CGCCATGGCT CCAAAGAAGA AGCGTAAGGT AAATCGATCG AATTCTGATG CAGAAGGTGT
  181 GTGGAGCCCC GACATTGAGC AGAGTTTTCA GGAGGCCCTG GCCATCTACC CTCCCTGTGG
  241 CCGTCGCAAG ATCATCCTGT CAGATGAGGG CAAGATGTAT GGCCGGAACG AACTCATTGC
  301 CCGTTACATC AAGCTGAGGA CAGGGAAGAC GAGAACGCGA AAGCAGGTCT CCAGCCATAT
  361 TCAGGTTTTG GCTCGAAGGA AATCGAGAGA AATTCAGTCC AAGCTGAAGG ACCAAGTCTC
  421 CATCGATGCC CTGGAGGATC GCTGCAGCCC ACAGTCAGCG CCCAGCCCCA TTACCCTACA
  481 AATGCAGCAT CTTCACCACC AGCAACAGCA GCAGCAGCAA CAGCAGCAGC AAATGCAGCA
  541 CCTCCACCAG CTGCAGCAAC TGCAGCAGTT GCACCAACAG CAACTGGCCG CCGGTGTCTT
  601 CCACCATCCG GCAATGGCCT TCGATGCCGC TGCAGCCGCC GCTGCTGCAG CTGCTGCTGC
  661 GGCCGCCCAC GCTCATGCTG CTGCACTGCA GCAGCGCCTC AGTGGCAGTG GATCGCCCGC
  721 ATCCTGCTCC ACGCNCGCCT CGTNCACGCC GCTGANCATC AAGGANGANG AAAGCGACTC
  781 CGTGATCGGN GACATGANTT TCCNCAATCA GANGCACACC ACCNNCGAGG ANNANGANGN
  841 GGANGANNNN GANNNNNTTG NTGTGGNNGN GGNNGANNNN TNNNNGGNNN GNNNNNCNNN
901 NNNNNNCCCN NNNNNNNNCA NCNNNNNNNN GNNNNNNNNN N
//
Primer: IRES-R (Pr0037)
Region: IRES,insert 3'(Myc,EnR 3')
Sequence file: RDB19900_B3Bab.seq check
>12175_19900_B3Ba_3_IRES-R_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANGTTANGG GGGGGGGNNG GNNNNGGGGG GGGGGGGCGG
   61 AATTTACGTA GCGGCCGCGG CGCGCCGGCC CTCGAGGCCT GCAGGAATTC CCGTACCACC
  121 ACACTGGGAT CCCTAGTTCA GGTCCTCCTC GGAGATCAGC TTCTGCTCAA GCTTGGGCTG
  181 CATAGATCCC AGAGCAGATT TCTCTGGATT GGCATCGCTC ATCTTGGAGC TCATGGGCGG
  241 CGGGATGACG GCATTGGCTG CACTCCTGCG CAGGCGACTC TGCACGATTT CCTCGTGTAT
  301 CCTGGGATAG GCAGCCGGAT TGAAGCAGTT AAGGAAGTTG GCCCGTTCCA GCATCATGGC
  361 CGCAGTAGCG GCTGCGGCGG CAGCTTGCTG TTGCCGGCTA AACTCCAGCA GATCCACTCT
  421 TGTAAAGGCG GAGGGCGTGG CAGTCTGGGA GCGATTCGCC TCGAAGGGGC GGAATATGCT
  481 GGCCTGGTTC TCAATCGACT TGCCAGGCTT CTGGACATCT CCGAAACGAT CGCTCAGGAT
  541 GTTGGAGATG GAAAAGGCCA GCGAGGGCTT GGCCGTCGAC TGCTGCTGGT GGGCGGGTGG
  601 TGGCAGGCGT CCGCCCGCCG ACGTATCATC CACATCCACA TCAATGTCGT CATCCTCCTC
  661 CGCCTCCTCC TCCTCGTTGG TGGTGTGCGT CTGATTGTGG AAACTCATGT CACCGNTCAC
  721 GGAGTCGCTT TCCTCCTCCT TGATGGTCAG CGGCGTGGAC GAGGCGGGCG TGGANCNNGA
  781 TGCGGGCGAT CCNCTGCCAC TGANGCGCTG CTGCANTGCA GCANCATGAG CGTGGGCGGC
  841 CGCNNCAGCA GCTGCANCAG CGGCGGCTGC AGCNNNNNNN ANNGNCANNG CCNGATGGTG
  901 GAANANNCCG NCGGCCAGTN GCNGTNGGNG CAACTGCTGC ANTTGCTNNA NNNNGNNNNN
961 NGNGCTGCNN TNNNTGCNNC NGNTNGCNNN NTNCNNNTGN TNNNNNNNNN N
//
Primer: IRES-F (Pr0036)
Region: IRES,EGFP 5'
Sequence file: RDB19900_B3Bac.seq check
>12175_19900_B3Ba_3_IRES-F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCGANGTT NNNNACGTCT AGGCCCCCCG AACCACGGGG
   61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCNAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCN TCGGCGANGG CCCCGTGCTG CTGCCCGACA ACCNCTACCT GANCACCCAG
  721 NCCGNCCTGA NCAAAGANCC NNNCGAGAAG CGCGATCNCN TGGNCCTGNT NGANTTCGTG
781 ACNGNCGNNN GNNNCACTCT CNNNNNNNNN N
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR,psi
Sequence file: RDB19900_B3Bad.seq check
>12175_19900_B3Ba_3_Amp_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNANTNNN NANTTATNNG GGTTATTGTC TCNTGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ANTGACTGAN
  601 TCGCCCGGGT ACCCGTATTC CNNATAAAGC CTCTTGCTGN TTGCNTCCGA ATCGTGNANT
  661 CGCTGATCCT TGGGANGGTC TCCTCANANT GANTGANTGN CCANCTCNGG GGNCNTTCNT
  721 TTGNAGGNTC CNCCGAGATT TNGNNANCCC TGNCCAGGGN NNNNNGANCC CCCCCGNCNG
  781 GNNGTAAGCN NGNNNANNNG NNCNTTTCNN GNCTGNCTCN GNNNTTGNNN NNGNNNNNNC
841 NGNCNTCTNN NNNTNGNNNN NNGNNNNNNN NNN
//
Primer: pMFG_1 (Pr0230)
Region: pol region,psi
Sequence file: RDB19900_B3Bae.seq check
>12175_19900_B3Ba_3_pMFG_1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNNNNNNNTC TTGTNNTGTC NGGGTCAGGG AAGTTTACAA
   61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
  121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
  181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
  241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
  301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
  361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
  421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
  481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA
  541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC
  601 GGCTTCNGTC CCAAACCGAA AGCAAAAATT CAGACGGANG CGGGAACTGT TTTANGTTCT
  661 CGTCTCCTAC CAGANCCACA TATCNNTCCT ATTANGGGGG TGCACCAAAG ANTCCNAAAC
  721 GATCGGGNNT TTTGGANTCN NNTCGGGCCN CAAAAACGGC CCCCGAANTC CCTGGGNNGT
  781 CNCCCNNGNT TGCNGNCNNG NNNNTNCNAA NNCNNCANTT NCANNNCGGN NNNNNCNNAN
841 NCNNANCTAG NTNANCTNNN TNNNACNNNN GCNNNNNNAN NNNNNNNNN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',3' LTR
Sequence file: RDB19900_B3Baf.seq check
>12175_19900_B3Ba_3_mVenus-M_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GCNTCNNGGT GNNNTTNNAG ANCCGCCNCA ACATCGAGGN
   61 NGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
  121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
  181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
  241 GGACGAGCTG TACAAGTAAA GCGGCCGGCC GCCAGCACAG TGGTCGACCG GGCCGCCAGC
  301 ACAGTGGTCG ATCGAGGGGG GGCCCGCGAT TAGTCCAATT TGTTAAAGAC AGGATATCAG
  361 TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT GAAGCCTATA GAGTACGAGC
  421 CATAGATAGA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA
  481 CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAATACATA
  541 ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG AGACAGCAGA ATATGGGCCA
  601 AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAN GGCCAAGAAC AGTTGGAACA
  661 GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCNNCGG CTCNNNNCCN
  721 AGAACAGATG GTCCCCAGAN GCGGTCCCGC CCTCAGCANT TNCTAGAGAA CCATCANANG
  781 NTTCCNGGGN GNCCCAAGGN NNNGAAATGA NCNNNNNNNN NATTTNAANT AANCNNNCAN
841 TTCGCTTNNC GCTTCNNNNC NNGNNCTTCN GNNNCCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]