Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMYs-Tead2-EnR-IRES-EGFP

Retroviral vector of mouse Tead2, dominant negative

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19900_B3Bap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(SV40 NLS,Tead2 1-127aa,EnR 5') Sequence file: RDB19900_B3Baa.seq
>12175_19900_B3Ba_3_MMLV-pol_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGNNCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCGC   121 CGCCATGGCT CCAAAGAAGA AGCGTAAGGT AAATCGATCG AATTCTGATG CAGAAGGTGT   181 GTGGAGCCCC GACATTGAGC AGAGTTTTCA GGAGGCCCTG GCCATCTACC CTCCCTGTGG   241 CCGTCGCAAG ATCATCCTGT CAGATGAGGG CAAGATGTAT GGCCGGAACG AACTCATTGC   301 CCGTTACATC AAGCTGAGGA CAGGGAAGAC GAGAACGCGA AAGCAGGTCT CCAGCCATAT   361 TCAGGTTTTG GCTCGAAGGA AATCGAGAGA AATTCAGTCC AAGCTGAAGG ACCAAGTCTC   421 CATCGATGCC CTGGAGGATC GCTGCAGCCC ACAGTCAGCG CCCAGCCCCA TTACCCTACA   481 AATGCAGCAT CTTCACCACC AGCAACAGCA GCAGCAGCAA CAGCAGCAGC AAATGCAGCA   541 CCTCCACCAG CTGCAGCAAC TGCAGCAGTT GCACCAACAG CAACTGGCCG CCGGTGTCTT   601 CCACCATCCG GCAATGGCCT TCGATGCCGC TGCAGCCGCC GCTGCTGCAG CTGCTGCTGC   661 GGCCGCCCAC GCTCATGCTG CTGCACTGCA GCAGCGCCTC AGTGGCAGTG GATCGCCCGC   721 ATCCTGCTCC ACGCNCGCCT CGTNCACGCC GCTGANCATC AAGGANGANG AAAGCGACTC   781 CGTGATCGGN GACATGANTT TCCNCAATCA GANGCACACC ACCNNCGAGG ANNANGANGN   841 GGANGANNNN GANNNNNTTG NTGTGGNNGN GGNNGANNNN TNNNNGGNNN GNNNNNCNNN 901 NNNNNNCCCN NNNNNNNNCA NCNNNNNNNN GNNNNNNNNN N //
Primer: IRES-R (Pr0037) Region: IRES,insert 3'(Myc,EnR 3') Sequence file: RDB19900_B3Bab.seq
>12175_19900_B3Ba_3_IRES-R_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NANGTTANGG GGGGGGGNNG GNNNNGGGGG GGGGGGGCGG    61 AATTTACGTA GCGGCCGCGG CGCGCCGGCC CTCGAGGCCT GCAGGAATTC CCGTACCACC   121 ACACTGGGAT CCCTAGTTCA GGTCCTCCTC GGAGATCAGC TTCTGCTCAA GCTTGGGCTG   181 CATAGATCCC AGAGCAGATT TCTCTGGATT GGCATCGCTC ATCTTGGAGC TCATGGGCGG   241 CGGGATGACG GCATTGGCTG CACTCCTGCG CAGGCGACTC TGCACGATTT CCTCGTGTAT   301 CCTGGGATAG GCAGCCGGAT TGAAGCAGTT AAGGAAGTTG GCCCGTTCCA GCATCATGGC   361 CGCAGTAGCG GCTGCGGCGG CAGCTTGCTG TTGCCGGCTA AACTCCAGCA GATCCACTCT   421 TGTAAAGGCG GAGGGCGTGG CAGTCTGGGA GCGATTCGCC TCGAAGGGGC GGAATATGCT   481 GGCCTGGTTC TCAATCGACT TGCCAGGCTT CTGGACATCT CCGAAACGAT CGCTCAGGAT   541 GTTGGAGATG GAAAAGGCCA GCGAGGGCTT GGCCGTCGAC TGCTGCTGGT GGGCGGGTGG   601 TGGCAGGCGT CCGCCCGCCG ACGTATCATC CACATCCACA TCAATGTCGT CATCCTCCTC   661 CGCCTCCTCC TCCTCGTTGG TGGTGTGCGT CTGATTGTGG AAACTCATGT CACCGNTCAC   721 GGAGTCGCTT TCCTCCTCCT TGATGGTCAG CGGCGTGGAC GAGGCGGGCG TGGANCNNGA   781 TGCGGGCGAT CCNCTGCCAC TGANGCGCTG CTGCANTGCA GCANCATGAG CGTGGGCGGC   841 CGCNNCAGCA GCTGCANCAG CGGCGGCTGC AGCNNNNNNN ANNGNCANNG CCNGATGGTG   901 GAANANNCCG NCGGCCAGTN GCNGTNGGNG CAACTGCTGC ANTTGCTNNA NNNNGNNNNN 961 NGNGCTGCNN TNNNTGCNNC NGNTNGCNNN NTNCNNNTGN TNNNNNNNNN N //
Primer: IRES-F (Pr0036) Region: IRES,EGFP 5' Sequence file: RDB19900_B3Bac.seq
>12175_19900_B3Ba_3_IRES-F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTCGANGTT NNNNACGTCT AGGCCCCCCG AACCACGGGG    61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC   601 TTCNAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCN TCGGCGANGG CCCCGTGCTG CTGCCCGACA ACCNCTACCT GANCACCCAG   721 NCCGNCCTGA NCAAAGANCC NNNCGAGAAG CGCGATCNCN TGGNCCTGNT NGANTTCGTG 781 ACNGNCGNNN GNNNCACTCT CNNNNNNNNN N //
Primer: Amp_R (Pr0008) Region: AmpR pro,5' LTR,psi Sequence file: RDB19900_B3Bad.seq
>12175_19900_B3Ba_3_Amp_R_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNANTNNN NANTTATNNG GGTTATTGTC TCNTGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT   181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG   241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ANTGACTGAN   601 TCGCCCGGGT ACCCGTATTC CNNATAAAGC CTCTTGCTGN TTGCNTCCGA ATCGTGNANT   661 CGCTGATCCT TGGGANGGTC TCCTCANANT GANTGANTGN CCANCTCNGG GGNCNTTCNT   721 TTGNAGGNTC CNCCGAGATT TNGNNANCCC TGNCCAGGGN NNNNNGANCC CCCCCGNCNG   781 GNNGTAAGCN NGNNNANNNG NNCNTTTCNN GNCTGNCTCN GNNNTTGNNN NNGNNNNNNC 841 NGNCNTCTNN NNNTNGNNNN NNGNNNNNNN NNN //
Primer: pMFG_1 (Pr0230) Region: pol region,psi Sequence file: RDB19900_B3Bae.seq
>12175_19900_B3Ba_3_pMFG_1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TNNNNNNNTC TTGTNNTGTC NGGGTCAGGG AAGTTTACAA    61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG   121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG   181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG   241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC   301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG   361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC   421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT   481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA   541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC   601 GGCTTCNGTC CCAAACCGAA AGCAAAAATT CAGACGGANG CGGGAACTGT TTTANGTTCT   661 CGTCTCCTAC CAGANCCACA TATCNNTCCT ATTANGGGGG TGCACCAAAG ANTCCNAAAC   721 GATCGGGNNT TTTGGANTCN NNTCGGGCCN CAAAAACGGC CCCCGAANTC CCTGGGNNGT   781 CNCCCNNGNT TGCNGNCNNG NNNNTNCNAA NNCNNCANTT NCANNNCGGN NNNNNCNNAN 841 NCNNANCTAG NTNANCTNNN TNNNACNNNN GCNNNNNNAN NNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',3' LTR Sequence file: RDB19900_B3Baf.seq
>12175_19900_B3Ba_3_mVenus-M_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GCNTCNNGGT GNNNTTNNAG ANCCGCCNCA ACATCGAGGN    61 NGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT   121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA   181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT   241 GGACGAGCTG TACAAGTAAA GCGGCCGGCC GCCAGCACAG TGGTCGACCG GGCCGCCAGC   301 ACAGTGGTCG ATCGAGGGGG GGCCCGCGAT TAGTCCAATT TGTTAAAGAC AGGATATCAG   361 TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT GAAGCCTATA GAGTACGAGC   421 CATAGATAGA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA   481 CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAATACATA   541 ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG AGACAGCAGA ATATGGGCCA   601 AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAN GGCCAAGAAC AGTTGGAACA   661 GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCNNCGG CTCNNNNCCN   721 AGAACAGATG GTCCCCAGAN GCGGTCCCGC CCTCAGCANT TNCTAGAGAA CCATCANANG   781 NTTCCNGGGN GNCCCAAGGN NNNGAAATGA NCNNNNNNNN NATTTNAANT AANCNNNCAN 841 TTCGCTTNNC GCTTCNNNNC NNGNNCTTCN GNNNCCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl