RDB12178 - pMYs-HA-Yap1 DTead BD-IRES-EGFP

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pMYs-HA-Yap1 DTead BD-IRES-EGFP (#RDB12178)

Retroviral vector of mouse Yap1, deletion of Tead BD

Clone info.	Retroviral vector of mouse Yap1, deletion of Tead binding domain (BD). cDNA fragments 210 to 299 nt and 513 to 1,625 nt of NM_009534.3 (CDS, 207..1625) are cloned (deletion of Tead binding domain).
Comment	Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). G to A substitution at 807 nt (Ala to Thr at 201st aa).
Vector backbone	pMYs-IRES-EGFP (Plasmid)
Size of vector backbone	6.1 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Yap1 cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.

Remarks Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks	Remember that you will be working with samples containing infections virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 MTAに書く付加的使用条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12178	pMYs-HA-Yap1 DTead BD-IRES-EGFP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pMYs-HA-Yap1 DTead BD-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12178).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12178_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') Sequence file: RDB12178_A6Aja.seq
>12178_12178_A6Aj_5'Retro(pFB-5')_A10_01.ab1 1 CGCCGCTAGG GGGTTTTTTC TTCGGACTGC TAGTTATTAA GGATCCATGT ACCCATACGA 61 CGTTCCAGAT TACGCTGGTA CCGACCCCGC GCAACAGCCG CCGCCCCAGC CGGCCCCGCA 121 AGGCCCCGCG CCGCCGTCCG TGTCTCCGGC CGGGACCCCC GCGGCCCCGC CCCTGACTCC 181 ACAGCATGTT CGAGCTCACT CCTCTCCAGC CTCCCTGCAG CTGGGTGCCG TTTCTCCTGG 241 GACACTCACA GCCAGTGGCG TTGTCTCTGG CCCTGCCGCT GCCCCTGCAG CTCAGCATCT 301 CCGGCAGTCC TCCTTTGAGA TCCCTGATGA TGTACCACTG CCAGCAGGCT GGGAGATGGC 361 CAAGACATCT TCTGGTCAAA GATACTTCTT AAATCACAAC GATCAGACAA CAACATGGCA 421 GGACCCCCGG AAGGCCATGC TTTCGCAACT GAACGTTCCT GCGCCTACCA GCCCAGCGGT 481 GCCCCAGACG CTGATGAATT CTGCCTCAGG ACCTCTTCCT GATGGATGGG AGCAAGCCAT 541 GACTCAGGAT GGAGAAGTTT ACTACATAAA CCATAAGAAC AAGACCACAT CCTGGCTGGA 601 CCCAAGGCTG GACCCTCGTT TTGCCATGAA CCAGAGGATC ACTCAGAGTG CTCCAGTGAA 661 GCAGCCCCCA CCCTTGGCTC CCCAGAGCCC ACAGGGAGGC GTCCTGGGTG GAGGCAGTTC 721 CAACCAGCAG CAGCAAATAC AGCTGCAGCA GTTACAGATG GAGAAGGAGA GACTGCGGTT 781 GAAACAACAG GAATTATTTC GGCAGGAATT AGCTCTGCGC AGCCAGTTGC CTACACTGGA 841 GCAGGATGGA GGGACTCCGA ATGCAGTGTC TTCTCCTGGG ATGTCTCAGG AATTGAGAAC 901 AATGACAACC AATAGTTCCG ATCCCTTTCT TAACAGTGGC ACCTATCACT CTCGAGATGA 961 GAGCACAGAC AGCGGCCTCA GCATGAGCAG CTACAGCATC CCTTCGGGAC CCCAGACGAC 1021 TTTCTCACAG TGTGATGAAA TGGATACAGG AGAACATCAT CAGCAAGCAC CCTTGCCGTC 1081 ACAGCAGAGT CGCTTCCCGA CTACCTGGAA GCTCTGGAAC AATGTGAACC TTGGCCACCA 1141 CCTGGAAAAG GAC //
Primer: IRES-R Sequence file: RDB12178_A6Ajb.seq
>12178_12178_A6Aj_IRES-R_C08_08.ab1 1 GCATGGCGGG GCTCGGCCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGGGCGG 61 AATTTACGTA GCGGCCGCCT ATAACCACGT GAGAAAGCTT TCTTTATCTA GCTTGGTGGC 121 AGCCAACACA GACTCCACGT CCAAGATTTC GGAACTCAGC GCTTCCTGCA GACTGGGCAT 181 CAGCTCCTCC CCTTCTATGT TCATTGCATC TCCTTCCAGT GTGCCAAGGT CCACATTTGT 241 CCCAGGGAGG GCTTCCAGGT AGTCGGGGAA GCGGCTCTGC TGTGACGGCA GGGTGCTTTG 301 GCTGATGGTG TCTCCTGTAT CCATTTCATC CACACTGTTG AGGAAGTCGT CTGGGGTCCG 361 AGGGATGCTG TAGCTGCTCA TGCTGAGGCC GCTGTCTGTG CTCTCATCTC GAGAGTGATA 421 GGTGCCACTG TTAAGAAAGG GATCGGAACT ATTGGTTGTC ATTGTTCTCA ATTCCTGAGA 481 CATCCCAGGA GAAGACACTG CATTCGGAGT CCCTCCATCC TGCTCCAGTG TAGGCAACTG 541 GCTGCGCAGA GCTAATTCCT GCCGAAATAA TTCCTGTTGT TTCAACCGCA GTCTCTCCTT 601 CTCCATCTGT AACTGCTGCA GCTGTATTTG CTGCTGCTGG TTGGAACTGC CTCCACCCAG 661 GACGCCTCCC TGTGGGCTCT GGGGAGCCAA GGGTGGGGGC TGCTTCACTG GAGCACTCTG 721 AGTGATCCTC TGGTTCATGG CAAACGAGGG TCCAGCCTTG GGTCCAGCCA GGATGTGGTC 781 TTGTTCTATG GTTATGTAGT AACTTCTCCA TCCTGGAGTC ATGCTGCTCC CATCATCAGA 841 AGAGGTCTGA GCAGAATCAT CACGTTCTGG GGCACCCGCT GGCCTGTAGG CCAGAACGTC 901 AGTGCGAGCC ATGGCCTCGG GTCCTGCATG TGTGTCTGGA TCGTTGGGAT CAGAGGATCT 961 CGACGAAGTG TCTGGCATTC AGCTGTGCAT GTACTTCAGG ATCCAGAACT GCGAATGCTC 1021 GA //
Primer: IRES-F Sequence file: RDB12178_A6Ajc.seq
>12178_12178_A6Aj_IRES-F_B10_04.ab1 1 GGCAATGCCT TTTAATGTGT TTAGTCGAGG TTAAAAAACG TCTAGGCCCC CCGAACCACG 61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG 121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG 601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG 841 CCAGCACAGT GGTCGACCGG GCCGCCAGCA CAGTGGTCGA TCGAGGGGGG GCCCGCGATT 901 AGTCCAATTT GTTAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA 961 TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGAATAGA ATAAAAAGAT TTTAATTAGT 1021 CCTCCAGAAA AAGGGGGGGA ATGAAAGACC CCCAACCTTG TAGTTTGGGC AAAGCTAGCT 1081 TAGGTAACGG CCAATTTTGC AAAGGCCGAT GGAAAATACA ATTAACCTGA AGAATGAGAG 1141 AAGTTCAGAA ATTCAAGGTT TTAGAGAC //
Primer: Amp_R Sequence file: RDB12178_A6Ajd.seq
>12178_12178_A6Aj_Amp_R_D08_11.ab1 1 ATCTTTTTAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT 181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG 241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT 301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA 361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC 421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG 481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC 541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG 601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT 661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT 721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG 781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG 841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC 901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAAGGG 961 ACTTCGGGGG CCGTTTTTTG TGGCCCGGAC CTGAAGTCCC AAAATTCCCG ATCGTTTTGG 1021 ACTTCTTTGG TGCACCCCTA TAGGAGGGAT ATGGTGGTTC TGGGTAGAGA CGAAGAACTT 1081 AAACAGTCCC GGCCTCCGTC CTGAATTATT GCTTTTCGAG TTTGGGGAAC CGGAAAAGG //
Primer: pMFG_1 Sequence file: RDB12178_A6Aje.seq
>12178_12178_A6Aj_pMFG-1_E08_14.ab1 1 GCGGGGAGAG GGCTGTTAGT ACTCTTGTCA TGTCAGGGTC AGGGAAGTTT ACAAGGGGCG 61 GGGGTGCCCC ATATAAGATC TCATATGGCC ATATGGGGGC GCCTAGAGAA GGAGTGAGGG 121 CTGGATAAAG GGAGGATCGA GGCGGGGTCG AACGAGGAGG TTCAAGGGGG AGAGACGGGG 181 CGGATGGAGG AAGAGGAGGC GGAGGCTTAG GGTGTACAAA GGGCTTGACC CAGGGAGGGG 241 GGTCAAAAGC CAAGGCTTCC CAGGTCACGA TGTAGGGGAC CTGGTCTGGG TGTCCATGCG 301 GGCCAGGTGA AAAGACCTTG ATCTTAACCT GGGTGATGAG GTCTCGGTTA AAGGTGCCGT 361 CTCGCGGCCA TCCGACGTTA AAGGTTGGCC ATTCTGCAGA GCAGAAGGTA ACCCAACGTC 421 TCTTCTTGAC ATCTACCGAC TGGTTGTGAG CGATCCGCTC GACATCTTTC CAGTGACCTA 481 AGGTCAAACT TAAGGGAGTG GTAACAGTCT GGCCCTAATT TTCAGACAAA TACAGAAACA 541 CAGTCAGACA GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC 601 GGTCCCAAAC CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC 661 CTACCAGAAC CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG 721 GATTTTTGGA CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA 781 GGGTTGCGGC CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC 841 TAGTTAGCTA ACTAGTACAG ACGCAGGCGC AAACATTAGA TGCCGGCACA AACACGCACA 901 AAGACAGAGA CAGACACGAA ACGACCGCTG GGCCAGCTTA CCTCCCGGCG GGGGGGTCGG 961 TGGTCCCTGG GCAGGGGTCT CCAATCTCGG TGGACTCGAA ATGAAGACCC CCGAGTGGGC 1021 AGTCCATTCA TCTGAAGAGA CCTCCAAGAT CAGCGAGTTC ACGATCAGAT GCACAGCAGA 1081 GCCTTTATGG AATACGGTTA CCCGGGCGCA //
Primer: EGFP-C Sequence file: RDB12178_A6Ajf.seq
>12178_12178_A6Aj_EGFP-C_F08_17.ab1 1 CAGGGCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG 61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT 121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA 181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG 241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA 301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA 361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC 421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT 481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC 541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT 601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC 661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC 721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT 781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCACATGCAG 841 CATGTATCAA AATTAATTTG GTTTTTTTTC TTAAGTATTT ACATTAAATG GCCATAGTTG 901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGG TATCAGCTCA 1021 CTCAAAGGCG GGTAATACGG GTTATCCCAC AGAATTCAGG GGATTACGCA GAAAAGAACA 1081 TGTGGAGCCA AACGCCCAGG CCAAAGGGCC AGGAACCGTA AAAAAAAGGC TGC //

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References

Original, user report and related articles

original	Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

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mouse Yap1

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Mouse Yap1

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