Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMYs-HA-Yap1 DWW-IRES-EGFP

Retroviral vector of mouse Yap1, deletion of WW domain

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12179_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') Sequence file: RDB12179_A6Aja.seq
>12179_12179_A6Aj_5'Retro(pFB-5')_G10_19.ab1     1 TTCCGCTTGA GAGGTTTCTC GGACTAGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG    61 GAATTCCTGC AGGCCATGTA CCCATACGAC GTTCCAGATT ACGCTGGTAC CGACCCCGCG   121 CAACAGCCGC CGCCCCAGCC GGCCCCGCAA GGCCCCGCGC CGCCGTCCGT GTCTCCGGCC   181 GGGACCCCCG CGGCCCCGCC CGCACCCCCG GCCGGCCACC AGGTCGTGCA CGTCCGCGGG   241 GACTCGGAGA CCGACTTGGA GGCGCTCTTC AATGCCGTCA TGAACCCCAA GACGGCCAAC   301 GTGCCTCAGA CCGTGCCCAT GCGGCTTCGC AAGCTGCCCG ACTCCTTCTT CAAGCCGCCT   361 GAGCCCAAGT CCCACTCGAG ACAGGCCAGT ACTGATGCAG GTACTGCGGG AGCTCTGACT   421 CCACAGCATG TTCGAGCTCA CTCCTCTCCA GCCTCCCTGC AGCTGGGTGC CGTTTCTCCT   481 GGGACACTCA CAGCCAGTGG CGTTGTCTCT GGCCCTGCCG CTGCCCCTGC AGCTCAGCAT   541 CTCCGGCAGT CCTCCTTTGA GATCCCTGAT GATGTACCAC TGCTGGACCC TCGTTTTGCC   601 ATGAACCAGA GGATCACTCA GAGTGCTCCA GTGAAGCAGC CCCCACCCTT GGCTCCCCAG   661 AGCCCACAGG GAGGCGTCCT GGGTGGAGGC AGTTCCAACC AGCAGCAGCA AATACAGCTG   721 CAGCAGTTAC AGATGGAGAA GGAGAGACTG CGGTTGAAAC AACAGGAATT ATTTCGGCAG   781 GAATTAGCTC TGCGCAGCCA GTTGCCTACA CTGGAGCAGG ATGGAGGGAC TCCGAATGCA   841 GTGTCTTCTC CTGGGATGTC TCAGGAATTG AGAACAATGA CAACCAATAG TTCCGATCCC   901 TTTCTTAACA GTGGCACCTA TCACTCTCGA GATGAGAGCA CAGACAGCGG GCCTCAGCAT   961 GAGCAGCTAC AGCATCCCTC GGACCCCAGA CGACTTTCTC ACAGTGTGGA TGAATGGATA  1021 CAGGAGACAC CATCAGCCAA GCACCCTTGC GTCACAGCAG AGCCGCTTCC CCGGACTACT  1081 GAGCCTCCCT GGGACAATGT GGACCTTGCC ACTGAGGAAT GCCATGACAT AGAGGGGGGG 1141 AGGGAGCTGT GATGC //
Primer: IRES-R Sequence file: RDB12179_A6Ajb.seq
>12179_12179_A6Aj_IRES-R_G08_20.ab1     1 GCATAGGCGG GCTTCGGGCC AGTACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGGGC    61 GGAATTTACG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC TCTATAACCA CGTGAGAAAG   121 CTTTCTTTAT CTAGCTTGGT GGCAGCCAAC ACAGACTCCA CGTCCAAGAT TTCGGAACTC   181 AGCGCTTCCT GCAGACTGGG CATCAGCTCC TCCCCTTCTA TGTTCATTGC ATCTCCTTCC   241 AGTGTGCCAA GGTCCACATT TGTCCCAGGG AGGGCTTCCA GGTAGTCGGG GAAGCGGCTC   301 TGCTGTGACG GCAGGGTGCT TTGGCTGATG GTGTCTCCTG TATCCATTTC ATCCACACTG   361 TTGAGGAAGT CGTCTGGGGT CCGAGGGATG CTGTAGCTGC TCATGCTGAG GCCGCTGTCT   421 GTGCTCTCAT CTCGAGAGTG ATAGGTGCCA CTGTTAAGAA AGGGATCGGA ACTATTGGTT   481 GTCATTGTTC TCAATTCCTG AGACATCCCA GGAGAAGACA CTGCATTCGG AGTCCCTCCA   541 TCCTGCTCCA GTGTAGGCAA CTGGCTGCGC AGAGCTAATT CCTGCCGAAA TAATTCCTGT   601 TGTTTCAACC GCAGTCTCTC CTTCTCCATC TGTAACTGCT GCACCTGTAT TTGCTGCTGC   661 TGGTTGGAAC TGCCTCCACC CAGGACGCCT CCCTGTGGGC TCTGGGGAGC CAAGGGTGGG   721 GGCTGCTTTC ACTGGAGCAC TCTGAGTGAT CCTCTGGTTC ATGGCAAAAA CGAGGGTCCA   781 GCAGTGGTAC ATCATCAGGG GATCTCAAAG GAAGGACTGC CGGAGATGCT GAGCTGCAAG   841 GGGCAGCGGC AAGGGCCCAG AAGACAAACG CCACTGGCTG TGAGTGTCCC AAGAAAAAAC   901 GGCACCCCAG CTGCAGGGAA GGCTGGGAAA GGAAGTGGAG CTTCGAACAT GCTTGTGGAA   961 GTCAAAGCCT CCCGCAATAC CTTGCATCCA TTACTTGGCC CTGTCCTCCG ATGGGGAATT  1021 TTGGGCTTCA GGCGGCTTGA AAAAAAGGAA TCCGGGCAGC TTGTCAACAC CGCCATAGGG 1081 CACACGGGTC TTAAAGGCAC ACAT //
Primer: IRES-F Sequence file: RDB12179_A6Ajc.seq
>12179_12179_A6Aj_IRES-F_H08_23.ab1     1 GAATTCCTTT TATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG    61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG   121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG   181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT   241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT   301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT   361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG   421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA   481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA   541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA   601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA   661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA   721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT   781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GGCCGGCCGC   841 CAGCACAGTG GTCGACCGGG CCGCCAGCAC AGTGGTCGAT CGAGGGGGGG CCCGCGATTA   901 GTCCAATTTG TTAAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA   961 TCACCAGCTG AAGCCCTATA GAGTACGAGC CCATAGATAG AATAAAAGAT TTTTATTTAA  1021 GTCTCCAAGA AAAAAGGGGG GGAATGAAAG AACCCCCACC TGTTAGGGTT GGGCAAGCCT  1081 AGCCTAAGTT AACGGCCATT TGGCCAAAGG CCATGAAAAT ACATACCTGA GAAATTAGAG 1141 AAAGCT //
Primer: Amp_R Sequence file: RDB12179_A6Ajd.seq
>12179_12179_A6Aj_Amp_R_A09_03.ab1     1 TTTGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT   181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG   241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG   601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT   661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT   721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG   781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG   841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC   901 CCGTGGTGGA AACTGACGAG TTCGGAACAC CCGGGCCGCA ACCCTGGGAG ACGTCCCAGG   961 GACTTCGGGG GCCGTTTTTG TGGCCGACCT GAGTCCAAAA TCCGATCGTT TTGGACTCTT  1021 TGGTGCACCC CCCTATAGAA GGGATATGTG GCTCCTGTTA GAGACGAGAC TAACAGTCCG 1081 GCTCGGTCTG ACTTTTGCTT CGGTTGGGAC GAAG //
Primer: pMFG_1 Sequence file: RDB12179_A6Aje.seq
>12179_12179_A6Aj_pMFG-1_B09_06.ab1     1 GGAAAGGGAA AGGAGGGGCT GTTAGTAACT CTTGTCATGT CAGGGTCAGG GAAGTTTACA    61 AGGGGCGGGG GTGCCCCATA TAAGATCTCA TATGGCCATA TGGGGGCGCC TAGAGAAGGA   121 GTGAGGGCTG GATAAAGGGA GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA   181 GACGGGGCGG ATGGAGGAAG AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG   241 GGAGGGGGGT CAAAAGCCAA GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT   301 CCATGCGGGC CAGGTGAAAA GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG   361 GTGCCGTCTC GCGGCCATCC GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC   421 CAACGTCTCT TCTTGACATC TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG   481 TGACCTAAGG TCAAACTTAA GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC   541 AGAAACACAG TCAGACAGAG ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG   601 CGGCTTCGGT CCCAAACCGA AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC   661 TCGTCTCCTA CCAGAACCAC ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA   721 CGATCGGGAT TTTTGGACTC AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG   781 TCTCCCAGGG TTGCGGCCGG GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT   841 ACAGAGCTAG TTAGCTAACT AGTACAGACG CAGGCGCAAA CATTAGATGC CGGCACAAAC   901 ACGCACAAAG ACAGAGACAG ACACGAAACG ACCGCTGGGC CAGCTTACCC TCCCGGCGGG   961 GGGGGTCGGT GGTCCCTGGG CAGGGGGTCT CCAAATCTCG GGTGGAACCT CCGAATGAAA  1021 GAACCCCCGA GGTGGGCAAG TCAATCAATC TGAGGAGACC CCTCCCAAGA TCAAGCCGAA  1081 GTCCACGATT TCGGATGCAT AGCAAGCAGC CTTTTATGGG ATTACCGGTT ACCCCGGGGG 1141 CGAACT //
Primer: EGFP-C Sequence file: RDB12179_A6Ajf.seq
>12179_12179_A6Aj_EGFP_C_D02_11.ab1     1 CAAGTGTTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT    61 CGACCGGGCC GCCAGCACAG TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT   121 AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG   181 CCTATAGAGT ACGAGCCATA GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG   241 GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG   301 GCATGGAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC   361 AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC   421 AAGAACAGTT GGAACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG   481 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT   541 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT   601 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA   661 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG   721 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC   781 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC   841 ATGTATCAAA ATTAATTTGG TTTTTTTTTC TTAAGTATTT ACATTAAATG GCCCATAGTT   901 GCATTAATGA ATCGGCCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG   961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC  1021 CACTCAAAGG CGGTATACGT TATCCACAGA TCAGGGATAC GCAGAAAGAA CATGTGAGCA 1081 AGGCAGCAAG TAAGACCGTA AAAAAGGTCC G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl