RDB12179 - pMYs-HA-Yap1 DWW-IRES-EGFP

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pMYs-HA-Yap1 DWW-IRES-EGFP (#RDB12179)

Retroviral vector of mouse Yap1, deletion of WW domain

Clone info.	Retroviral vector of mouse Yap1, deletion of WW domain. cDNA fragments 210 to 681 nt and 947 to 1,625 nt of NM_009534.3 (CDS, 207..1625) are cloned.
Comment	Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone	pMYs-IRES-EGFP (Plasmid)
Size of vector backbone	7.1 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Yap1 cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.

Remarks Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks	Remember that you will be working with samples containing infections virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 MTAに書く付加的使用条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12179	pMYs-HA-Yap1 DWW-IRES-EGFP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pMYs-HA-Yap1 DWW-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12179).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12179_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') Sequence file: RDB12179_A6Aja.seq
>12179_12179_A6Aj_5'Retro(pFB-5')_G10_19.ab1 1 TTCCGCTTGA GAGGTTTCTC GGACTAGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG 61 GAATTCCTGC AGGCCATGTA CCCATACGAC GTTCCAGATT ACGCTGGTAC CGACCCCGCG 121 CAACAGCCGC CGCCCCAGCC GGCCCCGCAA GGCCCCGCGC CGCCGTCCGT GTCTCCGGCC 181 GGGACCCCCG CGGCCCCGCC CGCACCCCCG GCCGGCCACC AGGTCGTGCA CGTCCGCGGG 241 GACTCGGAGA CCGACTTGGA GGCGCTCTTC AATGCCGTCA TGAACCCCAA GACGGCCAAC 301 GTGCCTCAGA CCGTGCCCAT GCGGCTTCGC AAGCTGCCCG ACTCCTTCTT CAAGCCGCCT 361 GAGCCCAAGT CCCACTCGAG ACAGGCCAGT ACTGATGCAG GTACTGCGGG AGCTCTGACT 421 CCACAGCATG TTCGAGCTCA CTCCTCTCCA GCCTCCCTGC AGCTGGGTGC CGTTTCTCCT 481 GGGACACTCA CAGCCAGTGG CGTTGTCTCT GGCCCTGCCG CTGCCCCTGC AGCTCAGCAT 541 CTCCGGCAGT CCTCCTTTGA GATCCCTGAT GATGTACCAC TGCTGGACCC TCGTTTTGCC 601 ATGAACCAGA GGATCACTCA GAGTGCTCCA GTGAAGCAGC CCCCACCCTT GGCTCCCCAG 661 AGCCCACAGG GAGGCGTCCT GGGTGGAGGC AGTTCCAACC AGCAGCAGCA AATACAGCTG 721 CAGCAGTTAC AGATGGAGAA GGAGAGACTG CGGTTGAAAC AACAGGAATT ATTTCGGCAG 781 GAATTAGCTC TGCGCAGCCA GTTGCCTACA CTGGAGCAGG ATGGAGGGAC TCCGAATGCA 841 GTGTCTTCTC CTGGGATGTC TCAGGAATTG AGAACAATGA CAACCAATAG TTCCGATCCC 901 TTTCTTAACA GTGGCACCTA TCACTCTCGA GATGAGAGCA CAGACAGCGG GCCTCAGCAT 961 GAGCAGCTAC AGCATCCCTC GGACCCCAGA CGACTTTCTC ACAGTGTGGA TGAATGGATA 1021 CAGGAGACAC CATCAGCCAA GCACCCTTGC GTCACAGCAG AGCCGCTTCC CCGGACTACT 1081 GAGCCTCCCT GGGACAATGT GGACCTTGCC ACTGAGGAAT GCCATGACAT AGAGGGGGGG 1141 AGGGAGCTGT GATGC //
Primer: IRES-R Sequence file: RDB12179_A6Ajb.seq
>12179_12179_A6Aj_IRES-R_G08_20.ab1 1 GCATAGGCGG GCTTCGGGCC AGTACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGGGC 61 GGAATTTACG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC TCTATAACCA CGTGAGAAAG 121 CTTTCTTTAT CTAGCTTGGT GGCAGCCAAC ACAGACTCCA CGTCCAAGAT TTCGGAACTC 181 AGCGCTTCCT GCAGACTGGG CATCAGCTCC TCCCCTTCTA TGTTCATTGC ATCTCCTTCC 241 AGTGTGCCAA GGTCCACATT TGTCCCAGGG AGGGCTTCCA GGTAGTCGGG GAAGCGGCTC 301 TGCTGTGACG GCAGGGTGCT TTGGCTGATG GTGTCTCCTG TATCCATTTC ATCCACACTG 361 TTGAGGAAGT CGTCTGGGGT CCGAGGGATG CTGTAGCTGC TCATGCTGAG GCCGCTGTCT 421 GTGCTCTCAT CTCGAGAGTG ATAGGTGCCA CTGTTAAGAA AGGGATCGGA ACTATTGGTT 481 GTCATTGTTC TCAATTCCTG AGACATCCCA GGAGAAGACA CTGCATTCGG AGTCCCTCCA 541 TCCTGCTCCA GTGTAGGCAA CTGGCTGCGC AGAGCTAATT CCTGCCGAAA TAATTCCTGT 601 TGTTTCAACC GCAGTCTCTC CTTCTCCATC TGTAACTGCT GCACCTGTAT TTGCTGCTGC 661 TGGTTGGAAC TGCCTCCACC CAGGACGCCT CCCTGTGGGC TCTGGGGAGC CAAGGGTGGG 721 GGCTGCTTTC ACTGGAGCAC TCTGAGTGAT CCTCTGGTTC ATGGCAAAAA CGAGGGTCCA 781 GCAGTGGTAC ATCATCAGGG GATCTCAAAG GAAGGACTGC CGGAGATGCT GAGCTGCAAG 841 GGGCAGCGGC AAGGGCCCAG AAGACAAACG CCACTGGCTG TGAGTGTCCC AAGAAAAAAC 901 GGCACCCCAG CTGCAGGGAA GGCTGGGAAA GGAAGTGGAG CTTCGAACAT GCTTGTGGAA 961 GTCAAAGCCT CCCGCAATAC CTTGCATCCA TTACTTGGCC CTGTCCTCCG ATGGGGAATT 1021 TTGGGCTTCA GGCGGCTTGA AAAAAAGGAA TCCGGGCAGC TTGTCAACAC CGCCATAGGG 1081 CACACGGGTC TTAAAGGCAC ACAT //
Primer: IRES-F Sequence file: RDB12179_A6Ajc.seq
>12179_12179_A6Aj_IRES-F_H08_23.ab1 1 GAATTCCTTT TATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG 61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG 121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG 181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT 241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT 301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT 361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG 421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA 541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA 601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA 661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA 721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT 781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GGCCGGCCGC 841 CAGCACAGTG GTCGACCGGG CCGCCAGCAC AGTGGTCGAT CGAGGGGGGG CCCGCGATTA 901 GTCCAATTTG TTAAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA 961 TCACCAGCTG AAGCCCTATA GAGTACGAGC CCATAGATAG AATAAAAGAT TTTTATTTAA 1021 GTCTCCAAGA AAAAAGGGGG GGAATGAAAG AACCCCCACC TGTTAGGGTT GGGCAAGCCT 1081 AGCCTAAGTT AACGGCCATT TGGCCAAAGG CCATGAAAAT ACATACCTGA GAAATTAGAG 1141 AAAGCT //
Primer: Amp_R Sequence file: RDB12179_A6Ajd.seq
>12179_12179_A6Aj_Amp_R_A09_03.ab1 1 TTTGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT 181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG 241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT 301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA 361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC 421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG 481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC 541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG 601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT 661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT 721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG 781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG 841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC 901 CCGTGGTGGA AACTGACGAG TTCGGAACAC CCGGGCCGCA ACCCTGGGAG ACGTCCCAGG 961 GACTTCGGGG GCCGTTTTTG TGGCCGACCT GAGTCCAAAA TCCGATCGTT TTGGACTCTT 1021 TGGTGCACCC CCCTATAGAA GGGATATGTG GCTCCTGTTA GAGACGAGAC TAACAGTCCG 1081 GCTCGGTCTG ACTTTTGCTT CGGTTGGGAC GAAG //
Primer: pMFG_1 Sequence file: RDB12179_A6Aje.seq
>12179_12179_A6Aj_pMFG-1_B09_06.ab1 1 GGAAAGGGAA AGGAGGGGCT GTTAGTAACT CTTGTCATGT CAGGGTCAGG GAAGTTTACA 61 AGGGGCGGGG GTGCCCCATA TAAGATCTCA TATGGCCATA TGGGGGCGCC TAGAGAAGGA 121 GTGAGGGCTG GATAAAGGGA GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA 181 GACGGGGCGG ATGGAGGAAG AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG 241 GGAGGGGGGT CAAAAGCCAA GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT 301 CCATGCGGGC CAGGTGAAAA GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG 361 GTGCCGTCTC GCGGCCATCC GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC 421 CAACGTCTCT TCTTGACATC TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG 481 TGACCTAAGG TCAAACTTAA GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC 541 AGAAACACAG TCAGACAGAG ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG 601 CGGCTTCGGT CCCAAACCGA AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC 661 TCGTCTCCTA CCAGAACCAC ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA 721 CGATCGGGAT TTTTGGACTC AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG 781 TCTCCCAGGG TTGCGGCCGG GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT 841 ACAGAGCTAG TTAGCTAACT AGTACAGACG CAGGCGCAAA CATTAGATGC CGGCACAAAC 901 ACGCACAAAG ACAGAGACAG ACACGAAACG ACCGCTGGGC CAGCTTACCC TCCCGGCGGG 961 GGGGGTCGGT GGTCCCTGGG CAGGGGGTCT CCAAATCTCG GGTGGAACCT CCGAATGAAA 1021 GAACCCCCGA GGTGGGCAAG TCAATCAATC TGAGGAGACC CCTCCCAAGA TCAAGCCGAA 1081 GTCCACGATT TCGGATGCAT AGCAAGCAGC CTTTTATGGG ATTACCGGTT ACCCCGGGGG 1141 CGAACT //
Primer: EGFP-C Sequence file: RDB12179_A6Ajf.seq
>12179_12179_A6Aj_EGFP_C_D02_11.ab1 1 CAAGTGTTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT 61 CGACCGGGCC GCCAGCACAG TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT 121 AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG 181 CCTATAGAGT ACGAGCCATA GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG 241 GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG 301 GCATGGAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC 361 AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 421 AAGAACAGTT GGAACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 481 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT 541 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 601 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 661 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG 721 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 781 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC 841 ATGTATCAAA ATTAATTTGG TTTTTTTTTC TTAAGTATTT ACATTAAATG GCCCATAGTT 901 GCATTAATGA ATCGGCCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 1021 CACTCAAAGG CGGTATACGT TATCCACAGA TCAGGGATAC GCAGAAAGAA CATGTGAGCA 1081 AGGCAGCAAG TAAGACCGTA AAAAAGGTCC G //

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References

Original, user report and related articles

original	Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

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mouse Yap1

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Mouse Yap1

Reference sequence