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pMYs-HA-Yap1 S112A-IRES-EGFP (#RDB12181)

Retroviral vector of mouse Yap1, S112A mutant

Clone info. Retroviral vector of mouse Yap1, S112A mutation in Tead BD. cDNA fragment 210 to 1,625 nt of NM_009534.3 (CDS, 207..1625) is cloned. T to G substitution at 540 nt and C to A substitution at 542 nt (Ser to Ala at 112th aa, introduced mutation).
Comment Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12181 pMYs-HA-Yap1 S112A-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-HA-Yap1 S112A-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12181).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19902_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(T7 pro,HA,Yap1 5')
Sequence file: RDB19902_B3Baa.seq check
>12181_19902_B3Ba_2_MMLV-pol_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGNAN CTTGGNNACN CGCCGCCCAC GTGAAGGCTG
   61 CCGACCCCGG GGGTGGACCA TCCTCTAGAC TGCCGGATCT AGCTAGTTAA TTAAGGATCC
  121 CAGTGTGGTG GTACGGGAAT TCCTGCAGGG GGCTAGCGAA TTCGATTAAT ACGACTCACT
  181 ATAGGGAGAC CCAAGCTTGC CGCCACCATG TACCCATACG ACGTTCCAGA TTACGCTGGT
  241 ACCGACCCCG CGCAACAGCC GCCGCCCCAG CCGGCCCCGC AAGGCCCCGC GCCGCCGTCC
  301 GTGTCTCCGG CCGGGACCCC CGCGGCCCCG CCCGCACCCC CGGCCGGCCA CCAGGTCGTG
  361 CACGTCCGCG GGGACTCGGA GACCGACTTG GAGGCGCTCT TCAATGCCGT CATGAACCCC
  421 AAGACGGCCA ACGTGCCTCA GACCGTGCCC ATGCGGCTTC GCAAGCTGCC CGACTCCTTC
  481 TTCAAGCCGC CTGAGCCCAA GTCCCACTCG AGACAGGCCA GTACTGATGC AGGTACTGCG
  541 GGAGCTCTGA CTCCACAGCA TGTTCGAGCT CACGCATCTC CAGCCTCCCT GCAGCTGGGT
  601 GCCGTTTCTC CTGGGACACT CACAGCCAGT GGCGTTGTCT CTGGCCCTGC CGCTGCCCCT
  661 GCAGCTCAGC ATCTCCGGCA GTCCTCCTTT GAGATCCCTG ATGATGTACC ACTGCNAGCA
  721 NGCTGGGANA TGGCCAAGAC ATCTTCTGGT CNNAGATACT TCTTAAATCA CAACGATCAG
  781 ACAACNACAT GGCANGANCC CCGGANNGNC ATGCTTTCGC AACTGAACGT TCCTGCGCCT
  841 GCCAGCCCAN CGGNGCCCCA GANGCTGATG NATTCTGNCN NNNGNANCNC TNNNNGATGG
  901 ANGGNNNNNN GNCNATGNNT CNNNATGGAN AANTTTACTA CNTNANNCAT NNNNNNNNAN
961 NNCNTNNNNN NNNGNNNNNN NNNN
//
Primer: Yap1-B_R (Pr1299)
Region: insert 3'(Yap1 3'),IRES
Sequence file: RDB19902_B3Bab.seq check
>12181_19902_B3Ba_2_Yap1-B_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AGTTCCGATC CCTTTCTTAA CAGTGGCACC
   61 TATCACTCTC GAGATGANAG CACAGACAGC GGCCTCAGCA TGAGCAGCTA CAGCATCCCT
  121 CGGACCCCAG ACGACTTCCT CAACAGTGTG GATGAAATGG ATACAGGAGA CACCATCAGC
  181 CAAAGCACCC TGCCGTCACA GCAGAGCCGC TTCCCCGACT ACCTGGAAGC CCTCCCTGGG
  241 ACAAATGTGG ACCTTGGCAC ACTGGAAGGA GATGCAATGA ACATAGAAGG GGAGGAGCTG
  301 ATGCCCAGTC TGCAGGAAGC GCTGAGTTCC GAAATCTTGG ACGTGGAGTC TGTGTTGGCT
  361 GCCACCAAGC TAGATAAAGA AAGCTTTCTC ACGTGGTTAT AGAGCTGCAG GGAGCCACTC
  421 TGAGTCTGTG AGGGATCCCT CGAGGGCCGG CGCGCCGCGG CCGCTACGTA AATTCCGCCC
  481 CCCCCCCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAANCCGCT TGGAATAAGG
541 NCGGNGTGCG TTTGTCTATA TGTNATTTTC CACCATATTG NCNNNNNNNN NN
//
Primer: IRES-F (Pr0036)
Region: IRES,EGFP 5'
Sequence file: RDB19902_B3Bac.seq check
>12181_19902_B3Ba_2_IRES-F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCTAGGCCC CCCGAACCAC
   61 GGGGACGTGG TTTTCNCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCNTCNAGG
  601 TGAACTTCNA GATCCGNNAC AACATCGAGG ANGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGANCAC CCCCATCGGN NACGGNCCCG NGCTGNTGCC CGANAACCAC TACCTGANCA
  721 CCCAGTCNGC CCTGANCAAA GANCCCNACG ANNANCGCGA TNACNTGGTC CTGCTGNANT
  781 TCNTGNNNGN NNNNNGNNTC NCTCTCNGNN TNNNNNANCT GNACNNGNAA NGNNGNNNGN
841 NNNNNANCNN NNNGNNCNAN NGNNNNNNNN NN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR,psi
Sequence file: RDB19902_B3Bad.seq check
>12181_19902_B3Ba_2_Amp_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNANTGN NNCNNTTANN NGGGTTNNTG TCTCNTGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGCATA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT
  181 TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAAAAG TTCAGATCAA GGTCAGGAAC
  241 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
  301 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
  361 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
  421 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
  481 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
  541 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG
  601 AGTCGCCCGG GTACCCGTAT TCCCAATAAA GCCTCTTGCT GTTTGCATCC GAATCGTGGA
  661 CTCGCTGATC CTTGGGAGGG TCTCCTCAGA TTGATTGACT GCCCACCTCG GGGGTCTTTC
  721 ATTTGGAGGT TCCACCGANA TTTGGAGACC CCTGCCCAGG GACCACCGAC CCCCCCGCCG
  781 GGNNGTAAGC TGGCCAGCGG TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGNNTGTGCC
  841 GGCATCTAAN GTTTGCGCCT GCGTCTGTAC TANTTANCNN NNTANCTCTG TATCTGNNGG
  901 ANCCGNGNNN NNANNNNNNA NTNCNNNNCN CCCNGNNNNC NNNCNNNGGN NNNGTCCNNN
961 NNNNNNN
//
Primer: pMFG_1 (Pr0230)
Region: pol region,psi
Sequence file: RDB19902_B3Bae.seq check
>12181_19902_B3Ba_2_pMFG_1_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNTNANTNNN TCTTGTNNTG TCNGGGTCAG GGAAGTTTAC
   61 AAGGGGCGGG GGTGCCCCNT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG
  121 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG
  181 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA
  241 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG
  301 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA
  361 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC
  421 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA
  481 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA
  541 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
  601 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA NGCGGGAACT GTTTTAGGTT
  661 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA
  721 ACGATCGGGA NTTTTGGANT CNNGTCGGGN CACAAAAACG GCCCCCGAAG TCCNTGGGAN
  781 GTCTCCCNNG GTTGCGGNCG GGNGTTNCGA ACTCGTCAGT TNCACCACNG GNNNNNNANA
  841 NNCNGANCTA GTTAGNTAAC TAGTACAGAN GCNNNNNNAA ACNTTANNTN NCNGGNNCAA
901 ACNCNCNNNA GNNNNANANN GNNNCNAAAC GNNNNNNNNN N
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',3' LTR
Sequence file: RDB19902_B3Baf.seq check
>12181_19902_B3Ba_2_mVenus-M_F_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTGNN NTTCNAGANC CGCCACAACA TCGAGGACGG
   61 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
  121 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
  181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
  241 CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG TCGACCGGGC CGCCAGCACA
  301 GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT TAAAGACAGG ATATCAGTGG
  361 TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA GCCTATAGAG TACGAGCCAT
  421 AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT
  481 GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA ATACATAACT
  541 GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA CAGCAGAATA TGGGCCAAAC
  601 AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC CAAGAACAGT TGGAACAGCA
  661 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC ANGGCCAAGA
  721 ACAGATGGTC CCCAGATGCN GNCCCGCCCT CAGCAGTTTC TAGAGAANNN TCANATGTTT
  781 CCAGGGNGNC CCAAGGACCT GAAATGACCC NGTGCCTTAT TTGANCTNAN NNATCAGTTC
841 GCTNCNCGCT TCTGNTCNCG CGCTNCTGNT CCCCNANCTN NATAAAANAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]