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pMYs-HA-dnYap1-IRES-EGFP (#RDB12182)

Retroviral vector of mouse Yap1, dominant negative

Alternative name pMYs-IG-HA-Yap1 S112A-DAD-EnR
Clone info. Retroviral vector of mouse Yap1, dominant negative. cDNA fragments 210 to 1,055 nt and 1,590 to 1,622 nt of NM_009534.3 (CDS, 207..1625) are cloned.
Comment S112A mutation, deletion of activation domain, Engrailed repression domain. Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position. Myc-tag is inserted downstream of the Yap1.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA Drosophila engrailed cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12182 pMYs-HA-dnYap1-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-HA-dnYap1-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12182).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19903_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(HA,Yap1 S112A 5')
Sequence file: RDB19903_B3Baa.seq check
>12182_19903_B3Ba_2_MMLV-pol_F_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNTCGNANCT NGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCAT
  121 GTACCCATAC GACGTTCCAG ATTACGCTGG TACCGACCCC GCGCAACAGC CGCCGCCCCA
  181 GCCGGCCCCG CAAGGCCCCG CGCCGCCGTC CGTGTCTCCG GCCGGGACCC CCGCGGCCCC
  241 GCCCGCACCC CCGGCCGGCC ACCAGGTCGT GCACGTCCGC GGGGACTCGG AGACCGACTT
  301 GGAGGCGCTC TTCAATGCCG TCATGAACCC CAAGACGGCC AACGTGCCTC AGACCGTGCC
  361 CATGCGGCTT CGCAAGCTGC CCGACTCCTT CTTCAAGCCG CCTGAGCCCA AGTCCCACTC
  421 GAGACAGGCC AGTACTGATG CAGGTACTGC GGGAGCTCTG ACTCCACAGC ATGTTCGAGC
  481 TCACGCATCT CCAGCCTCCC TGCAGCTGGG TGCCGTTTCT CCTGGGACAC TCACAGCCAG
  541 TGGCGTTGTC TCTGGCCCTG CCGCTGCCCC TGCAGCTCAG CATCTCCGGC AGTCCTCCTT
  601 TGAGATCCCT GATGANGTAC CACTGCCAGC AGGCTGGGAG ANGGNCNAGA CATCTTCTGG
  661 TCAAAGATAC TTCTTAAATC ACAACGATCA GACAACAACA TGGCANGNNC CCCGGAANGN
  721 CATGCTTTCG CAACTGNNNG TTCCTGCNCC TGNCAGCCCA GCGGNGCCCC AGACGNTGAT
  781 GANTTCTGCN TCNNANNNCT TNNTGANGNA TGGGNNCNNG CCNNNACTCN NNNNGANAAN
841 TTTNCTANNT AAANNNTNNN ANCNNGANNN NNTNNNNNNN NNANCNNNNN NNNNN
//
Primer: EnR-M_R (Pr1298)
Region: insert 3'(EnR 3',Myc),IRES
Sequence file: RDB19903_B3Bab.seq check
>12182_19903_B3Ba_2_EnR-M_R_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCGNNNNN NCGAGGCGAA TCGCTCCCNG ACTGCCNCGC
   61 CCTCCGCCTT TACNAGAGTG GATCTGCTGG AGTTTAGCCG GCAACAGCAA GCTGCCGCCG
  121 CAGCCGCTAC TGCGGCCATG ATGCTGGAAC GGGCCAACTT CCTTAACTGC TTCAATCCGG
  181 CTGCCTATCC CAGGATACAC GAGGAAATCG TGCAGAGTCG CCTGCGCAGG AGTGCAGCCA
  241 ATGCCGTCAT CCCGCCGCCC ATGAGCTCCA AGATGAGCGA TGCCAATCCA GAGAAATCTG
  301 CTCTGGGATC TATGCAGCCC AAGCTTGAGC AGAAGCTGAT CTCCGAGGAG GACCTGAACT
  361 AGGGATCCCA GTGTGGTGGT ACGGGAATTC CTGCAGGCCT CGAGGGCCGG CGCGCCGCGG
  421 CCGCTACGTA AATTCCGCCC CCCCCCCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC
  481 CNAANCNNNN NGGAATAAGG NNNNNGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG
  541 CCGTCTTTTG GCNANTGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA NNANNNTTNC
  601 NTAGGGGTCN TTTCCCCTCT CNCCAAAGGA ANNGCAAGGN NTGTTNANNG NCNTNNANNG
661 NANGNAGTTN NCNTCTGNAA NCTNNNTGAA NACNANNNNN NNNNN
//
Primer: Yap-M_F (Pr0286)
Region: insert mid(Yap1 S112A,EnR 5')
Sequence file: RDB19903_B3Bac.seq check
>12182_19903_B3Ba_2_Yap-M_F_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNNCNTNNN NNNCTGANCG TTCCTGCGCC TGCCNGCCCN GCGGTGCCCC
   61 AGACGCTGAT GAATTCTGCC TCAGGACCTC TTCCTGATGG ATGGGAGCAA GCCATGACTC
  121 AGGATGGAGA AGTTTACTAC ATAAACCATA AGAACAAGAC CACATCCTGG CTGGACCCAA
  181 GGCTGGACCC TCGTTTTGCC ATGAACCAGA GGATCACTCA GAGTGCTCCA GTGAAGCAGC
  241 CCCCACCCTT GGCTCCCCAG AGCCCACAGG GAGGCGTCCT GGGTGGAGGC AAGCTAGATA
  301 AAGAAAGCTT TCTCACGTGG TTAATCGATG CCCTGGAGGA TCGCTGCAGC CCACAGTCAG
  361 CGCCCAGCCC CATTACCCTA CAAATGCAGC ATCTTCACCA CCAGCAACAG CAGCAGCAGC
  421 AACAGCAGCA GCAAATGCAG CACCTCCACC AGCTGCAGCA ACTGCAGCAG TTGCACCAAC
  481 AGCAACTGGC CGCCGGTGTC TTCCACCATC CGGCAATGGC CTTCGATGCC GCTGCAGCCG
  541 CCGCTGCTGC AGCTGCTGCT GCGGCCGCCC ACGCTCATGC TGCTGCACTG CAGCAGCGCC
  601 TCAGTGGCAG TGGATCGCCC GCATCCTGCT CCACGCCCGC CTCGTCCACG CCGCTGACCA
  661 TCAAGGAGGA GGAAAGCGAC TCCGTGATCG GTGACATGAG TTTCCACAAT CAGACGCACA
  721 CNACCAACGA GGANGAGGAN GCGGANGAGG ANGACGACNT TGATGTGNAN GTGGATGATA
  781 CGTCGGCGGG CGGANGCCTG NCACCACCCG CCCACCAGCA GCAGTCGANG GNCAAGCCNN
  841 CGCTGGNNTT TTNNNTCTNC NNCNTCCNGA NCGATCGTTT CNNANANGNC CNNAANNNNN
  901 NNNNGNCNAN NGANNANCNN NNNNNGCATA NTNNNNCCNN NNNAGNNNAN NNNNNNCNNN
961 ANNNNNNNNN CNNNNNNNNN N
//
Primer: IRES-F (Pr0036)
Region: IRES,EFGP 5'
Sequence file: RDB19903_B3Bad.seq check
>12182_19903_B3Ba_2_IRES-F_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN GTCGANGTTN NNNNNNGTCT AGGCCCCCCG AACCACGGGG
   61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGANTTCGTG
  781 ANCGCCGCCG GGATCACTCT CGGCATGGAC GANCTGTNCA AGTAAAGCGG NCGGCCGCCA
  841 GCACAGNGGT CGANCGNGCC GCCNGCACAG NGGTCGATCN ANGGGGGGGC CCGCGANTAN
  901 TCCNATTNGN TAAAGANANG NNATCANNGG NCCNNGCTCT ANNTTNGANT CNNCNNNATC
961 ACCNGCTGNN NNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR
Sequence file: RDB19903_B3Bae.seq check
>12182_19903_B3Ba_2_Amp_R_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCANTT ATNNGGGTTA TTGTCTCATG AGCGGANACA
   61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
  121 TGCCACCTGC ATAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC
  181 ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAA AAGTTCAGAT CAAGGTCAGG
  241 AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC
  301 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT
  361 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC
  421 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC
  481 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC
  541 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATTGA
  601 CTGAGTCGCC CGGGTACCCG TATTCCCAAT AAAGCCTCTT GCTGTTTGCA TCCGAATCGT
  661 GGACTCGCTG ATCCTTGGGA GGGTCTCCTC AGATTGATTG ACTGNCCACC TCGGGGGTCT
  721 TTCNTTTGGA GGTTCCACCG ANATTTGGAG ANCCCTGCCC AGGGNNCACC NACCCCCCCG
  781 CCNGGANGNA AGCTGGNCAG CGGTCGTTTC GNGTCTGTCT CTGTCTTTGT GCGNGTTTGT
  841 GCCGGCATCT AANGNTNGNG CCTGCNTCTG TACTANNNAN CTNNNTANCT CTGNATCTGG
901 NNNANCNNNG NNNNNNNTNN NNANNNNNNN NNN
//
Primer: pMFG_1 (Pr0230)
Region: pol region,psi
Sequence file: RDB19903_B3Baf.seq check
>12182_19903_B3Ba_2_pMFG_1_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNNNN TNANNNNNTC TTGTNNTGTC NGGGTCAGGG NAGTTTACAA
   61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
  121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
  181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
  241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
  301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
  361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
  421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
  481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA
  541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC
  601 GGCTTCGGTC CCAAACCGAA AGCAAAAATT CAGACGGAGG CGGGAACTGT TTTAGGTTCT
  661 CGTCTCCTAC CAGAACCACA TATCCCTCCT ATTANGGGGG TGCACCNAAG AGTCCNAAAC
  721 GATCGGGNNT TNTGGANTCN NGTCGGGCCA CAAAAACNGC CCCCGAAGTC CCTGGGANGT
  781 CTCCCANGGT TGCGGCCGGG NGTTCCNAAN NCGNCNGTTC CNCCNCGGGT NCNCCNNATA
  841 CNNANCTANT TANCTNACTA NNACAGANGC NNNNGCAAAN NTTAGATGNN NGNNNNAANN
901 CNCNCNAAGN NNNNNNNNN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',3' LTR
Sequence file: RDB19903_B3Bag.seq check
>12182_19903_B3Ba_2_mVenus-M_F_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG CNTCNNGGTG NNNTTCNNGN NCCGCCACAA CATCGAGGAN
   61 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
  121 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG
  181 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG
  241 GACGAGCTGT ACAAGTAAAG CGGCCGGCCG CCAGCACAGT GGTCGACCGG GCCGCCAGCA
  301 CAGTGGTCGA TCGAGGGGGG GCCCGCGATT AGTCCAATTT GTTAAAGACA GGATATCAGT
  361 GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC
  421 ATAGATAGAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC
  481 CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAATACATAA
  541 CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA GACAGCAGAA TATGGGCCAA
  601 ACAGGATATC TGTGGTAAGC AGTTCCTGCN NCGGCTCNNG GCCNAGAACA GTTGGAACAG
  661 CAGAATATGG GCCAAACANG ANATCTGTGG TAAGCANNNN CTGCCCNGGC TCNNNNCNNA
  721 GAACAGANGG NCCCNNGATG CNNTCCCGCC CNNNNCAGTT TCTAGAGAAC CNTCNNANGT
  781 TTCCNGGGNN CCNCNANNNA NNTGAANNGA NCNNNNNNCN NNNNTTGNNN TAANCNNNNC
841 ANNNCNNTTN NCGNTTCNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]