Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMYs-HA-dnYap1-IRES-EGFP

Retroviral vector of mouse Yap1, dominant negative

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12182_A4Bip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') Sequence file: RDB12182_A4Bia.seq
>12182_12182_A4Bi_5'Retro_F04_16.ab1     1 AGGCATTTTC GTCGTGCCCG TTCGCGGACT GCTAGTTATT AAGGATCCAT GTACCCATAC    61 GACGTTCCAG ATTACGCTGG TACCGACCCC GCGCAACAGC CGCCGCCCCA GCCGGCCCCG   121 CAAGGCCCCG CGCCGCCGTC CGTGTCTCCG GCCGGGACCC CCGCGGCCCC GCCCGCACCC   181 CCGGCCGGCC ACCAGGTCGT GCACGTCCGC GGGGACTCGG AGACCGACTT GGAGGCGCTC   241 TTCAATGCCG TCATGAACCC CAAGACGGCC AACGTGCCTC AGACCGTGCC CATGCGGCTT   301 CGCAAGCTGC CCGACTCCTT CTTCAAGCCG CCTGAGCCCA AGTCCCACTC GAGACAGGCC   361 AGTACTGATG CAGGTACTGC GGGAGCTCTG ACTCCACAGC ATGTTCGAGC TCACGCATCT   421 CCAGCCTCCC TGCAGCTGGG TGCCGTTTCT CCTGGGACAC TCACAGCCAG TGGCGTTGTC   481 TCTGGCCCTG CCGCTGCCCC TGCAGCTCAG CATCTCCGGC AGTCCTCCTT TGAGATCCCT   541 GATGATGTAC CACTGCCAGC AGGCTGGGAG ATGGCCAAGA CATCTTCTGG TCAAAGATAC   601 TTCTTAAATC ACAACGATCA GACAACAACA TGGCAGGACC CCCGGAAGGC CATGCTTTCG   661 CAACTGAACG TTCCTGCGCC TGCCAGCCCA GCGGTGCCCC AGACGCTGAT GAATTCTGCC   721 TCAGGACCTC TTCCTGATGG ATGGGAGCAA GCCATGACTC AGGATGGAGA AGTTTACTAC   781 ATAAACCATA AGAACAAGAC CACATCCTGG CTGGACCCAA GGCTGGACCC TCGTTTTGCC   841 ATGAACCAGA GGATCACTCA GAGTGCTCCA GTGAAGCAGC CCCCACCCTT GGCTCCCCAG   901 AGCCCACAGG GAGGCGTCCT GTGTGGAGGC AAGCTAGATA AAGAAAGCTT TCTCACGTGG   961 TTAATCGATG CCCTGGAGGA TCGCTGCAGC CCACAGTCAG CGCCCAGCCC CATTACCCTA  1021 CAAATGCAGC ATCTTCACAC CAGCACAGCA GCAGCAGCAA CAGCAGCAGC AAATGCAGCA  1081 CTCACAGCTG CAGCACTGCC AGCAGTGCAC ACCAGCCACC TGCGCGTGTC TCACATCTGC 1141 AATGGCTTCG AATGCGCTTG CAGCCGGTCG GCTGACTTGC CAG //
Primer: IRES-R Sequence file: RDB12182_A4Bib.seq
>12182_12182_A4Bi_IRES-R_G04_19.ab1     1 GCCGGGCAGC GGGGCTCGGC AGTACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGGGC    61 GGAATTTACG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC CTGCAGGAAT TCCCGTACCA   121 CCACACTGGG ATCCCTAGTT CAGGTCCTCC TCGGAGATCA GCTTCTGCTC AAGCTTGGGC   181 TGCATAGATC CCAGAGCAGA TTTCTCTGGA TTGGCATCGC TCATCTTGGA GCTCATGGGC   241 GGCGGGATGA CGGCATTGGC TGCACTCCTG CGCAGGCGAC TCTGCACGAT TTCCTCGTGT   301 ATCCTGGGAT AGGCAGCCGG ATTGAAGCAG TTAAGGAAGT TGGCCCGTTC CAGCATCATG   361 GCCGCAGTAG CGGCTGCGGC GGCAGCTTGC TGTTGCCGGC TAAACTCCAG CAGATCCACT   421 CTTGTAAAGG CGGAGGGCGT GGCAGTCTGG GAGCGATTCG CCTCGAAGGG GCGGAATATG   481 CTGGCCTGGT TCTCAATCGA CTTGCCAGGC TTCTGGACAT CTCCGAAACG ATCGCTCAGG   541 ATGTTGGAGA TGGAAAAGGC CAGCGAGGGC TTGGCCGTCG ACTGCTGCTG GTGGGCGGGT   601 GGTGGCAGGC GTCCGCCCGC CGACGTATCA TCCACATCCA CATCAATGTC GTCATCCTCC   661 TCCGCCTCCT CCTCCTCGTT GGTGGTGTGC GTCTGATTGT GGAAACTCAT GTCACCGATC   721 ACGGAGTCGC TTTCCTCCTC CTTGATGGTC AGCGGCGTGG ACGAGGCGGG CGTGGAGCAG   781 GATGCGGGCG ATCCACTGCC ACTGAGGCGC TGCTGCAGTG CAGCAGCATG AGCGTGGGCG   841 GCCGCAGCAG CAGCTGCAGC AGCGGCGGCT GCAGCGGCAT CGAAGGCCAT TGCCGGATGG   901 TGGAAGACAC CGGCGGCCAG TTGCTGTTGG TGCAACTGCT GCAGTTGCTG CAGCTGGTGG   961 AGGTGCTGCA TTTGCTGCTG CTGTTGCTGC TGCTGCTGTT GCTGGTGGTG AAGATGCTGC  1021 ATTTGTAGGG TAATGGGGCT GGGCGCTGAC TGGTGGGCTG CAGCGATCCT CCAGGGCATC  1081 GATTTAACCA CGTGAGAAAG GCTTTCTTTA TCTAGCTTGC CTCCACCAAG GACGCCTCCT 1141 GTGGACTCTG GGGAGCCAAG GTGGCGGCTG CTCCTGAGCC ATCTGAGTGA TCCTCCTG //
Primer: IRES-F Sequence file: RDB12182_A4Bic.seq
>12182_12182_A4Bi_IRES_F_A03_03.ab1     1 TCAATCTTCC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA   121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA   181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA   241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC   301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA   361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA   421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT   481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA   541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT   601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA   661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC   721 CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC   781 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC GGGCCGCCAG   841 CACAGTGGTC GACCGGGCCG CCAGCACAGT GGTCGATCGA GGGGGGGCCC GCGATTAGTC   901 CAATTTGTTA AGACAGGATA TCAGTGGTCC AGGCTCTAGT TTTGACTCAA CAATATCACC   961 AGCTGAAGCC TTATAGAGTA CGAGCCCATA GATAGATAAG ATTTTTTATT TAGTCTCCAG  1021 AAAAAGGGGG AATGAAGGAC CCCCACCTGT AGTTTGGCAG CTAGCTAGTA CGCCATTGCC 1081 AGCCATGGTA AATTACGTTA ACCTGGAGCA TGAAGAAGGT C //
Primer: Amp_R Sequence file: RDB12182_A4Bid.seq
>12182_12182_A4Bi_Amp_R_B10_04.ab1     1 TTTATTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA   241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC   301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC   361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG   421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT   481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC   541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA   601 GTCGCCCGGG TACCCGTATT CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC   661 TCGCTGATCC TTGGGAGGGT CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA   721 TTTGGAGGTT CCACCGAGAT TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG   781 GAGGTAAGCT GGCCAGCGGT CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG   841 GCATCTAATG TTTGCGCCTG CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA   901 CCCGTGGTGG AACTGACGAG TTCGGGAACA CCCGGGCCGC ACCCTGGGGA GACGTCCAGG   961 ACTTCGGGGG CGTTTTTGTG GCCGACTGAG TCAAAATCCG ATCGTTTGGA CTCTTTGTGC  1021 ACCCCTTATA GAAGGATATG TGATCTGGTA AGAACGAGAC TCACAGTTCG CTCGCGACTT 1081 TTGCTTCGGA TTGGGACCGA GA //
Primer: pMFG_1 Sequence file: RDB12182_A4Bie.seq
>12182_12182_A4Bi_pMFG_1_B03_06.ab1     1 ACAAAGGGGA AGGAGGGCTG TTAGTAACTC TTGTCATGTC AGGGTCAGGG AAGTTTACAA    61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG   121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG   181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG   241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC   301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG   361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC   421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT   481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA   541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC   601 GGCTTCGGTC CCAAACCGAA AGCAAAAATT CAGACGGAGG CGGGAACTGT TTTAGGTTCT   661 CGTCTCCTAC CAGAACCACA TATCCCTCCT ATTAGGGGGG TGCACCAAAG AGTCCAAAAC   721 GATCGGGATT TTTGGACTCA GGTCGGGCCA CAAAAACGGC CCCCGAAGTC CCTGGGACGT   781 CTCCCAGGGT TGCGGCCGGG TGTTCCGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA   841 CAGAGCTAGT TAGCTAACTA GTACAGACGC AGGCGCAAAC ATTAGATGCC GGCACAAACA   901 CGCACAAAGA CAGAGACAGA CACGAAACGA CCGCTGGGCC AGCTTACCTC CCCGGCGGGG   961 GGGGTCGGTG GTCCCTGGCA GGGGTCTCCA AATCTCGGTG GAACCTCCAA TGAAAGACCC  1021 CCGAGGTGGG CAGTCAATCA ATCTGAGAGA CCCCTCCCAG GATCAGCGAG TTCCAACGAT 1081 CCGGATGCAC AGCAGAGGCC TTTATGGATA CGGGTACCCG GCCGAACTTC AAGT //
Primer: EGFP-C Sequence file: RDB12182_A4Bif.seq
>12182_12182_A4Bi_EGFP_C_C03_09.ab1     1 CCGGGGTCAC TCTCGGGCAT GGACGAGCTG TACAAGTAAA GCGGCCGGCC GCCAGCACAG    61 TGGTCGACCG GGCCGCCAGC ACAGTGGTCG ATCGAGGGGG GGCCCGCGAT TAGTCCAATT   121 TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT   181 GAAGCCTATA GAGTACGAGC CATAGATAGA ATAAAAGATT TTATTTAGTC TCCAGAAAAA   241 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG   301 CAAGGCATGG AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG   361 AGACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG   421 GGCCAAGAAC AGTTGGAACA GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT   481 CCTGCCCCGG CTCAGGGCCA AGAACAGATG GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT   541 TTCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA CCCTGTGCCT   601 TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG   661 CTCAATAAAA GAGCCCACAA CCCCTCACTC GGCGCGCCAG TCCTCCGATA GACTGCGTCG   721 CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG TGGTCTCGCT   781 GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT CTTTCACATG   841 CAGCATGTAT CAAAATTAAT TTGGTTTTTT TTTCTTAAGT ATTTACATTA AATGGCCATA   901 GTTGCATTAA TGAATCGGGC CAACGCGCGG GGAGAGGCGG GTTTGCGTAT TGGGCGCTCT   961 TCCGCTTCCT TCGCTCACTG ACTCGCTGCG CTCGGGTCGT TTCGGCTGCG GGCGAGCGGT  1021 ATCAGCTCAC TCAAAGCGGT AATAACGGTT TATCCCACAG AATCAAGGGA TTACGGCAAG 1081 AAAGAACATG TGAGCCAAGG CAGCCAAGCC AAGCAACCGT AAAAAAAGGT CTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl